ACTN3 (NM_001258371) Human Untagged Clone

CAT#: SC332676

ACTN3 (untagged) - Homo sapiens actinin, alpha 3 (ACTN3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACTN3
• Synonyms: ACTN3D
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001258371, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGATAGGGCTCTGTGTCCCACCAGCGAGGTGCTGGAAGAGGCTCACGAGGAGCTCCAGAGGGCAGC
  CTCTAGAGCGGCCCGCGGCCACTATTATTATCGGCCTTTCTATTGTGGAGAGGAGTAAACAGACTCAGAG
  AGGTCCCGTGGATTTCTCAAGGTCACACAGCGCCCCAGCAGCGGAGCAGGAATGCAAACCCAGGATCTCC
  GCCGCGAGATCCACCCCGGCGCCCCCGAGTCCCGCAGTTCCCGGCTTCAGGACAACCCCTTGCCAGACCT
  TCACTGCCTGGTGCAACTCACACCTGCGCAAGGCAGGCACCCAGATCGAGAACATCGAGGAAGATTTCCG
  CAATGGCCTCAAACTCATGCTGCTCCTGGAGGTCATTTCAGGTGAGAGGCTGCCTAGGCCAGATAAAGGC
  AAGATGCGCTTCCACAAAATCGCCAACGTTAACAAGGCCCTGGACTTCATTGCCAGCAAGGGGGTTAAAC
  TGGTGTCCATTGGTGCTGAAGAGATTGTTGACGGGAACCTGAAGATGACCCTGGGCATGATCTGGACCAT
  CATCCTTCGCTTCGCCATCCAGGACATCTCTGTGGAAGAAACCTCAGCCAAGGAAGGCTTGCTTCTGTGG
  TGCCAGAGGAAGACAGCACCGTACCGCAACGTCAACGTGCAGAACTTCCACACCAGCTGGAAGGATGGCC
  TGGCCCTCTGTGCCCTCATCCACCGACACCGCCCTGACCTCATCGACTACGCCAAACTGCGAAAGGATGA
  CCCCATCGGAAACCTGAACACTGCCTTTGAGGTGGCAGAGAAATACCTGGACATCCCCAAGATGTTGGAT
  GCAGAAGACATTGTGAACACCCCAAAGCCGGATGAGAAGGCCATCATGACCTATGTGTCCTGCTTCTACC
  ATGCCTTTGCCGGGGCTGAGCAGGCAGAGACAGCTGCCAACAGGATCTGCAAGGTGCTGGCAGTGAACCA
  GGAAAACGAGAAGCTGATGGAGGAGTATGAGAAGCTTGCCAGTGAGCTGCTGGAGTGGATCCGCCGCACT
  GTCCCATGGCTGGAGAACCGTGTGGGTGAGCCCAGCATGAGTGCCATGCAGCGCAAACTAGAGGACTTTC
  GGGACTACCGGCGTCTGCACAAGCCGCCCCGCATTCAGGAAAAGTGCCAGCTGGAGATCAACTTCAACAC
  ACTGCAGACCAAGTTGCGGCTCAGCCACCGGCCTGCCTTCATGCCCTCCGAGGGCAAGCTGGTCTCGGAC
  ATCGCCAACGCCTGGCGGGGGCTGGAGCAGGTGGAAAAGGGCTATGAGGACTGGCTGCTCTCGGAGATCC
  GGCGCCTGCAGCGACTCCAGCACCTGGCTGAGAAGTTCCGGCAGAAGGCCTCCCTGCACGAAGCCTGGAC
  CCGGGGAAAGGAGGAGATGCTGAGCCAGCGCGACTACGATTCGGCTTTGCTACAGGAGGTGCGGGCGTTG
  CTGCGGCGCCACGAGGCCTTTGAGAGCGACCTGGCGGCGCACCAGGACCGCGTGGAGCACATTGCCGCGC
  TGGCCCAGGAGCTCAATGAGCTGGACTACCACGAGGCAGCCTCAGTGAATAGCCGCTGCCAGGCCATCTG
  CGATCAGTGGGACAACCTGGGCACCCTGACCCAGAAGAGGCGGGATGCGCTAGAGCGGATGGAGAAGCTC
  CTGGAGACCATTGACCAGCTGCAACTGGAGTTTGCCCGGCGGGCCGCGCCCTTCAACAACTGGCTGGATG
  GTGCCGTGGAGGACCTGCAGGACGTGTGGCTGGTACACTCTGTGGAGGAGACCCAGAGCCTGCTGACAGC
  GCACGATCAGTTCAAGGCAACACTGCCCGAGGCTGACCGAGAGCGAGGTGCCATCATGGGCATCCAGGGT
  GAGATCCAGAAGATCTGCCAGACGTATGGGCTGCGGCCCTGCTCCACCAATCCCTACATCACCCTCAGCC
  CGCAGGACATCAACACCAAGTGGGATATGGTCCGAAAGCTGGTGCCCAGCCGTGACCAGACACTGCAGGA
  GGAGCTGGCACGGCAGCAGGTAAACGAGAGGCTCCGGCGACAGTTTGCGGCCCAGGCCAATGCCATTGGA
  CCCTGGATCCAGGCGAAGGTGGAGGAAGTGGGGCGGCTGGCAGCAGGGCTAGCTGGCTCTCTGGAGGAGC
  AGATGGCTGGGCTACGGCAGCAGGAGCAGAACATTATCAACTACAAGACTAACATTGACCGGCTGGAGGG
  TGACCACCAGCTGCTGCAGGAGAGCCTGGTGTTCGACAATAAGCACACCGTCTACAGCATGGAGCACATC
  CGCGTGGGCTGGGAGCAGCTGCTCACCTCCATTGCCCGCACCATCAATGAAGTGGAGAACCAGGTACTGA
  CCCGAGACGCCAAGGGACTGAGCCAGGAGCAGCTCAACGAGTTCCGAGCATCCTTCAACCACTTTGACAG
  GAAGCGGAATGGGATGATGGAGCCTGATGACTTCCGAGCTTGCCTCATCTCCATGGGCTATGACCTGGGG
  GAAGTGGAGTTTGCTCGCATCATGACCATGGTGGACCCCAACGCAGCTGGGGTGGTGACCTTCCAGGCCT
  TCATAGACTTCATGACCCGAGAGACAGCCGAGACTGACACGACTGAGCAAGTTGTAGCTTCCTTCAAGAT
  CTTGGCAGGAGACAAGAACTACATCACCCCCGAGGAGCTGCGGCGCGAGCTCCCTGCCAAGCAGGCCGAG
  TACTGCATCCGCCGTATGGTGCCCTACAAGGGATCCGGGGCCCCGGCTGGAGCCCTGGACTACGTGGCCT
  TCTCCAGTGCCCTCTATGGGGAGAGCGACCTTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001258371
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001258371.1, NP_001245300.1
• RefSeq Size: 3086 bp
• RefSeq ORF: 2835 bp
• Locus ID: 89
• Cytogenetics: 11q13.2
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Focal adhesion, Leukocyte transendothelial migration, Regulation of actin cytoskeleton, Systemic lupus erythematosus, Tight junction
• Gene Summary: 'This gene encodes a member of the alpha-actin binding protein gene family. The encoded protein is primarily expressed in skeletal muscle and functions as a structural component of sarcomeric Z line. This protein is involved in crosslinking actin containing thin filaments. An allelic polymorphism in this gene results in both coding and non-coding variants; the reference genome represents the coding allele. The non-functional allele of this gene is associated with elite athlete status. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (2) uses an alternate exon in the 5' UTR and coding region and initiates translation at an alternate start codon, compared to variant 1. The encoded isoform (2) is longer and has a distinct N-terminus, compared to isoform 1.