SRP72 (NM_001267722) Human Untagged Clone

CAT#: SC332875

SRP72 (untagged) - Homo sapiens signal recognition particle 72kDa (SRP72), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SRP72
• Synonyms: BMFF; BMFS1; HEL103
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001267722, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTATGGCCAGA
  ACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAACAAAGATGACGTAACTGC
  CCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAATACT
  CACACCAAAGTGTTAGCCAATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAA
  TTGAGAATGCCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGACA
  AGTGTTATACCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAA
  GATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGGGAAAAAG
  TGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAACACTGCATGTGCACTGAT
  AGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAAGCTGAAGATCTTTGCCGCCGTTCATTA
  TCAGAAGACACTGACCAAAATGTCTTTGACTCCAAGAAGAAAGTGAAATTAACCAATGCGGAAGGAGTAG
  AGTTTAAGCTTTCCAAGAAACAACTACAAGCTATAGAATTTAACAAAGCTTTACTTGCTATGTACACAAA
  CCAGGCTGAACAATGCCGCAAAATATCTGCCAGTTTACAGTCCCAAAGTCCCGAGCATCTCTTACCTGTG
  TTAATCCAAGCTGCCCAGCTCTGCCGTGAAAAGCAGCACACAAAAGCAATAGAGCTGCTTCAGGAATTTT
  CAGATCAGCATCCAGAAAATGCAGCTGAAATTAAGCTGACCATGGCACAGTTGAAAATTTCTCAAGGTAA
  TATTTCTAAAGCATGTCTAATATTGAGAAGCATAGAGGAGTTAAAGCATAAACCAGGCATGGTATCTGCA
  TTAGTTACCATGTATAGCCATGAAGAAGATATTGATAGTGCCATTGAGGTCTTCACACAAGCTATCCAGT
  GGTATCAAAACCATCAGCCAAAATCTCCTGCTCATTTGTCCTTGATAAGAGAAGCTGCAAACTTCAAACT
  CAAATATGGGCGGAAGAAGGAGGCAATTAGTGACCTACAACAGCTGTGGAAACAAAATCCAAAAGATATT
  CACACCCTGGCACAGCTTATTTCTGCTTACTCACTTGTAGATCCAGAGAAAGCCAAAGCTCTTAGTAAAC
  ACTTGCCATCGTCAGATAGTATGTCTCTAAAAGTAGATGTTGAGGCTCTTGAAAATTCTGCTGGTGCTAC
  ATACATTCGGAAGAAGGGTGGAAAAGTTACTGGAGATAGTCAACCAAAGGAACAAGGACAGGGAGATTTG
  AAAAAGAAGAAAAAGAAAAAGAAGGGAAAATTGCCTAAGAATTATGACCCAAAAGTTACCCCAGATCCAG
  AAAGATGGCTGCCAATGCGAGAACGTTCTTACTACCGGGGAAGAAAGAAGGGTAAAAAGAAGGATCAGAT
  TGGAAAAGGGACCCAGGGAGCAACTGCAGGAGCTTCATCTGAACTGGATGCCAGTAAAACTGTGAGCAGC
  CCACCCACCTCCCCAAGACCTGGCAGTGCTGCAACAGTATCTGCCTCTACAAGTAACATCATACCCCCAA
  GACACCAGAAACCTGCAGGGGCTCCAGCAACAAAAAAGAAACAGCAACAGAAAAAGAAGAAAGGTGGAAA
  AGGTGGCTGGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001267722
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267722.1, NP_001254651.1
• RefSeq Size: 3710 bp
• RefSeq ORF: 1833 bp
• Locus ID: 6731
• Cytogenetics: 4q12
• Protein Pathways: Protein export
• Gene Summary: 'This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]'
  Transcript Variant: This variant (2) lacks 2 consecutive in-frame coding exons in the 5' region compared to variant 1, which results in a shorter isoform (2) missing an internal protein segment compared to isoform 1.