SRP72 (NM_001267722) Human Tagged ORF Clone

CAT#: RC234559

SRP72 (Myc-DDK tagged) - Homo sapiens signal recognition particle 72kDa (SRP72), transcript variant 2

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SRP72
• Synonyms: BMFF; BMFS1; HEL103
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC234559 representing NM_001267722
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGAGCGGCGGCAGCGGGGGGGTGTCAGTACCTGCGCTGTGGAGTGAAGTGAACCGGTATGGCCAGA
  ACGGCGACTTCACGCGCGCTCTCAAGACCGTCAATAAGATACTACAGATCAACAAAGATGACGTAACTGC
  CCTGCATTGTAAAGTGGTATGCCTTATCCAGAATGGAAGTTTCAAGGAAGCTTTGAATGTCATCAATACT
  CACACCAAAGTGTTAGCCAATAACTCTCTCTCCTTTGAAAAGGCATATTGCGAGTACAGGCTGAACAGAA
  TTGAGAATGCCTTGAAGACAATAGAAAGTGCCAACCAGCAGACAGACAAACTGAAGGAGCTTTATGGACA
  AGTGTTATACCGTTTGGAACGCTATGATGAATGCTTAGCAGTGTATAGAGATCTCGTCCGAAACTCCCAA
  GATGATTATGATGAGGAGAGGAAAACAAACCTTTCAGCAGTTGTTGCAGCTCAAAGCAATTGGGAAAAAG
  TGGTTCCAGAGAACCTGGGCCTCCAAGAAGGCACACATGAGCTGTGCTACAACACTGCATGTGCACTGAT
  AGGCCAAGGCCAGCTGAACCAGGCCATGAAAATCCTACAAAAAGCTGAAGATCTTTGCCGCCGTTCATTA
  TCAGAAGACACTGACCAAAATGTCTTTGACTCCAAGAAGAAAGTGAAATTAACCAATGCGGAAGGAGTAG
  AGTTTAAGCTTTCCAAGAAACAACTACAAGCTATAGAATTTAACAAAGCTTTACTTGCTATGTACACAAA
  CCAGGCTGAACAATGCCGCAAAATATCTGCCAGTTTACAGTCCCAAAGTCCCGAGCATCTCTTACCTGTG
  TTAATCCAAGCTGCCCAGCTCTGCCGTGAAAAGCAGCACACAAAAGCAATAGAGCTGCTTCAGGAATTTT
  CAGATCAGCATCCAGAAAATGCAGCTGAAATTAAGCTGACCATGGCACAGTTGAAAATTTCTCAAGGTAA
  TATTTCTAAAGCATGTCTAATATTGAGAAGCATAGAGGAGTTAAAGCATAAACCAGGCATGGTATCTGCA
  TTAGTTACCATGTATAGCCATGAAGAAGATATTGATAGTGCCATTGAGGTCTTCACACAAGCTATCCAGT
  GGTATCAAAACCATCAGCCAAAATCTCCTGCTCATTTGTCCTTGATAAGAGAAGCTGCAAACTTCAAACT
  CAAATATGGGCGGAAGAAGGAGGCAATTAGTGACCTACAACAGCTGTGGAAACAAAATCCAAAAGATATT
  CACACCCTGGCACAGCTTATTTCTGCTTACTCACTTGTAGATCCAGAGAAAGCCAAAGCTCTTAGTAAAC
  ACTTGCCATCGTCAGATAGTATGTCTCTAAAAGTAGATGTTGAGGCTCTTGAAAATTCTGCTGGTGCTAC
  ATACATTCGGAAGAAGGGTGGAAAAGTTACTGGAGATAGTCAACCAAAGGAACAAGGACAGGGAGATTTG
  AAAAAGAAGAAAAAGAAAAAGAAGGGAAAATTGCCTAAGAATTATGACCCAAAAGTTACCCCAGATCCAG
  AAAGATGGCTGCCAATGCGAGAACGTTCTTACTACCGGGGAAGAAAGAAGGGTAAAAAGAAGGATCAGAT
  TGGAAAAGGGACCCAGGGAGCAACTGCAGGAGCTTCATCTGAACTGGATGCCAGTAAAACTGTGAGCAGC
  CCACCCACCTCCCCAAGACCTGGCAGTGCTGCAACAGTATCTGCCTCTACAAGTAACATCATACCCCCAA
  GACACCAGAAACCTGCAGGGGCTCCAGCAACAAAAAAGAAACAGCAACAGAAAAAGAAGAAAGGTGGAAA
  AGGTGGCTGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC234559 representing NM_001267722
  Red=Cloning site Green=Tags(s)
  
  MASGGSGGVSVPALWSEVNRYGQNGDFTRALKTVNKILQINKDDVTALHCKVVCLIQNGSFKEALNVINT
  HTKVLANNSLSFEKAYCEYRLNRIENALKTIESANQQTDKLKELYGQVLYRLERYDECLAVYRDLVRNSQ
  DDYDEERKTNLSAVVAAQSNWEKVVPENLGLQEGTHELCYNTACALIGQGQLNQAMKILQKAEDLCRRSL
  SEDTDQNVFDSKKKVKLTNAEGVEFKLSKKQLQAIEFNKALLAMYTNQAEQCRKISASLQSQSPEHLLPV
  LIQAAQLCREKQHTKAIELLQEFSDQHPENAAEIKLTMAQLKISQGNISKACLILRSIEELKHKPGMVSA
  LVTMYSHEEDIDSAIEVFTQAIQWYQNHQPKSPAHLSLIREAANFKLKYGRKKEAISDLQQLWKQNPKDI
  HTLAQLISAYSLVDPEKAKALSKHLPSSDSMSLKVDVEALENSAGATYIRKKGGKVTGDSQPKEQGQGDL
  KKKKKKKKGKLPKNYDPKVTPDPERWLPMRERSYYRGRKKGKKKDQIGKGTQGATAGASSELDASKTVSS
  PPTSPRPGSAATVSASTSNIIPPRHQKPAGAPATKKKQQQKKKKGGKGGW
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001267722
• ORF Size: 1830 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001267722.1, NP_001254651.1
• RefSeq Size: 3710 bp
• RefSeq ORF: 1833 bp
• Locus ID: 6731
• Cytogenetics: 4q12
• Protein Pathways: Protein export
• MW: 68.3 kDa
• Gene Summary: 'This gene encodes the 72 kDa subunit of the signal recognition particle (SRP), a ribonucleoprotein complex that mediates the targeting of secretory proteins to the endoplasmic reticulum (ER). The SRP complex consists of a 7S RNA and 6 protein subunits: SRP9, SRP14, SRP19, SRP54, SRP68, and SRP72, that are bound to the 7S RNA as monomers or heterodimers. SRP has at least 3 distinct functions that can be associated with the protein subunits: signal recognition, translational arrest, and ER membrane targeting by interaction with the docking protein. Mutations in this gene are associated with familial bone marrow failure. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2012]'