ISG20 (NM_001303235) Human Untagged Clone

CAT#: SC334135

ISG20 (untagged) - Human interferon stimulated exonuclease gene 20kDa (ISG20), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ISG20
• Synonyms: CD25; HEM45
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001303235, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAACTATCAGGTGTGAGGAGGGTCCTAGTGCAATCTCTTCTTTTCAAAGATGATACATTTGAAGCCC
  AGGGAGGACTGATAATTTGCCCAAGGTCACACAGCCAGTCAAGGAGGGATTGCTCCCTTGCCAGCCCCAG
  CCCCACCCAGAAGATGCTGGGTCATGTAGGGGTGGGCACATGTCTTCCTGGCCAGATCCTGCAGCTCCTG
  AAAGGCAAGCTGGTGGTGGGTCATGACCTGAAGCACGACTTCCAGGCACTGAAAGAGGACATGAGCGGCT
  ACACAATCTACGACACGTCCACTGACAGGCTGTTGTGGCGTGAGGCCAAGCTGGACCACTGCAGGCGTGT
  CTCCCTGCGGGTGCTGAGTGAGCGCCTCCTACACAAGAGCATCCAGAACAGCCTGCTTGGACACAGCTCG
  GTGGAAGATGCGAGGGCAACGATGGAGCTCTATCAAATCTCCCAGAGAATCCGAGCCCGCCGAGGGCTGC
  CCCGCCTGGCTGTGTCAGACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001303235
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001303235.1, NP_001290164.1
• RefSeq Size: 3070 bp
• RefSeq ORF: 513 bp
• Locus ID: 3669
• Cytogenetics: 15q26.1
• Gene Summary: ''
  Transcript Variant: This variant (4) has multiple differences in the 5' UTR and 5' coding region, and initiates translation at an alternate start codon compared to variant 1. The resulting isoform (b) has a shorter and distinct N-terminus compared to isoform a. Variants 4 and 5 both encode the same isoform (b). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.