TRA2A (NM_001282759) Human Untagged Clone

CAT#: SC334216

TRA2A (untagged) - Human transformer 2 alpha homolog (Drosophila) (TRA2A), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRA2A
• Synonyms: AWMS1; HSU53209
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334216 representing NM_001282759.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTAACCGGAGAAGACATACTGGCAGCAGGGCAAATCCAGATCCCAACACTTGCCTTGGAGTGTTT
  GGCCTCAGTTTGTACACAACAGAGAGGGATCTTCGTGAAGTATTTTCTCGATATGGACCATTGAGTGGT
  GTCAATGTGGTTTATGATCAGCGAACTGGGCGATCTCGAGGATTTGCTTTTGTGTATTTTGAGAGAATA
  GATGACTCAAAGGAGGCTATGGAAAGGGCAAATGGAATGGAGCTGGATGGTAGAAGAATTCGGGTGGAT
  TATTCTATAACCAAGAGAGCGCACACACCAACACCAGGCATCTACATGGGCAGACCAACTCATAGTGGT
  GGGGGTGGTGGAGGAGGCGGCGGCGGTGGAGGTGGAGGTGGTGGCAGACGTCGAGATTCTTACTATGAT
  AGAGGATATGATCGTGGGTATGACAGATATGAAGACTATGATTACCGATACAGACGATCACCTTCTCCT
  TATTATAGTCGATATAGATCACGATCAAGATCTCGTTCCTACAGCCCAAGACGCTATTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001282759
• Insert Size: 543 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282759.1
• RefSeq Size: 2254 bp
• RefSeq ORF: 543 bp
• Locus ID: 29896
• UniProt ID: Q13595
• Cytogenetics: 7p15.3
• Protein Pathways: Spliceosome
• MW: 20.6 kDa
• Gene Summary: This gene is a member of the transformer 2 homolog family and encodes a protein with several RRM (RNA recognition motif) domains. This phosphorylated nuclear protein binds to specific RNA sequences and plays a role in the regulation of pre-mRNA splicing. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Sep 2013]
  Transcript Variant: This variant (4) contains two additional exons in the 5' region and uses an alternate in-frame acceptor splice site in the 3' coding region compared to variant 1. The former difference results in translation initiation from an in-frame downstream start codon, and an isoform (3) with a shorter N-terminus compared to isoform 1.