DYRK4 (NM_001282285) Human Untagged Clone

CAT#: SC334674

DYRK4 (untagged) - Human dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 4 (DYRK4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DYRK4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334674 representing NM_001282285.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGAGCCTGGGCTGCATCACGGCGGAGTTGTACACGGGCTACCCCCTGTTCCCCGGGGAGAATGAG
  GTGGAGCAGCTGGCCTGCATCATGGAGGTGCTGGGTCTGCCGCCAGCCGGCTTCATTCAGACAGCCTCC
  AGGAGACAGACATTCTTTGATTCCAAAGGTTTTCCTAAAAATATAACCAACAACAGGGGGAAAAAAAGA
  TACCCAGATTCCAAGGACCTCACGATGGTGCTGAAAACCTATGACACCAGCTTCCTGGACTTTCTCAGA
  AGGTGTTTGGTATGGGAACCTTCTCTTCGCATGACCCCGGACCAGGCCCTCAAGCATGCTTGGATTCAT
  CAGTCTCGGAACCTCAAGCCACAGCCCAGGCCCCAGACCCTGAGGAAATCCAATTCCTTTTTCCCCTCT
  GAGACAAGGAAGGACAAGGTTCAAGGCTGTCATCACTCGAGCAGAAAAGCAGATGAGATCACCAAAGAG
  ACTACAGAGAAAACAAAAGATAGCCCCACGAAGCATGTTCAGCATTCAGGTGATCAGCAGGACTGTCTC
  CAGCACGGAGCTGACACTGTTCAGCTGCCTCAACTGGTAGACGCTCCCAAGAAGTCAGAGGCAGCTGTC
  GGGGCGGAGGTGTCCATGACCTCCCCAGGACAGAGCAAAAACTTCTCCCTCAAGAACACAAACGTTTTA
  CCCCCTATTGTATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001282285
• Insert Size: 705 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282285.1
• RefSeq Size: 968 bp
• RefSeq ORF: 705 bp
• Locus ID: 8798
• Cytogenetics: 12p13.32
• Protein Families: Druggable Genome, Protein Kinase
• MW: 26.4 kDa
• Gene Summary: This gene encodes an enzyme that belongs to a conserved family of serine/threonine protein kinases. Members of this dual specificity kinase family are thought to function in the regulation of cell differentiation and proliferation, survival, and in development. Alternate splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) differs in the 5' UTR and represents the use of an alternate promoter which results in translation initiation at a downstream AUG compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1.