HSPE1-MOB4 (NM_001202485) Human Untagged Clone

CAT#: SC334897

HSPE1 (untagged) - Human HSPE1-MOB4 readthrough (HSPE1-MOB4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSPE1-MOB4
• Synonyms: HSPE1-PHOCN
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334897 representing NM_001202485.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCAGGACAAGCGTTTAGAAAGTTTCTTCCACTCTTTGACCGAGTATTGGTTGAAAGGAGTGCTGCT
  GAAACTGTAACCAAAGGAGGCATTATGCTTCCAGAAAAATCTCAAGGAAAAGTATTGCAAGCAACAGTA
  GTCGCTGTTGGATCGGGTTCTAAAGGAAAGGATTTCTATAATTGGCCTGATGAATCCTTTGATGAAATG
  GACAGTACACTAGCTGTTCAACAGTATATTCAACAGAACATAAGAGCAGATTGCTCCAATATTGACAAA
  ATTCTTGAACCACCTGAAGGCCAAGATGAAGGTGTGTGGAAGTATGAACATTTAAGGCAGTTCTGCCTT
  GAGCTAAATGGACTTGCTGTCAAACTTCAGAGTGAATGCCATCCAGATACTTGCACTCAAATGACAGCA
  ACTGAACAATGGATTTTTCTTTGTGCAGCTCATAAAACTCCAAAAGAGTGTCCTGCTATAGACTATACT
  AGACACACACTTGATGGTGCTGCATGTCTTCTGAATAGCAATAAATATTTTCCCAGCAGGGTTAGCATA
  AAGGAATCATCTGTAGCGAAACTAGGATCAGTATGCCGTAGGATTTACAGAATATTTTCACATGCTTAT
  TTTCATCATCGGCAGATATTTGATGAATATGAAAATGAAACATTTTTGTGTCATCGGTTTACTAAGTTT
  GTGATGAAATACAATTTGATGTCCAAGGATAACCTGATTGTACCAATTTTAGAAGAGGAAGTACAGAAT
  TCAGTTTCTGGGGAAAGTGAAGCATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001202485
• Insert Size: 786 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001202485.1
• RefSeq Size: 4279 bp
• RefSeq ORF: 786 bp
• Locus ID: 100529241
• Cytogenetics: 2q33.1
• MW: 29.7 kDa
• Gene Summary: This locus represents naturally occurring read-through transcription between the neighboring HSPE1 (heat shock 10kDa protein 1 (chaperonin 10)) and MOB4 (MOB family member 4, phocein) genes on chromosome 2. The read-through transcript produces a fusion protein that shares sequence identity with each individual gene product. [provided by RefSeq, Oct 2011]