CDKL1 (NM_001282236) Human Untagged Clone

CAT#: SC334996

CDKL1 (untagged) - Human cyclin-dependent kinase-like 1 (CDC2-related kinase) (CDKL1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDKL1
• Synonyms: KKIALRE; P42
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC334996 representing NM_001282236.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGGAGAAGTATGAAAAAATTGGGAAAATTGGAGAAGGATCCTATGGAGTTGTTTTCAAATGTAGA
  AACAGGGACACGGGTCAGATTGTGGCCATCAAGAAGTTTCTGGAATCAGAAGATGACCCTGTCATAAAG
  AAAATTGCCCTTCGGGAAATCCGAATGCTCAAGCAACTCAAGCATCCCAACCTTGTTAACCTCCTGGAA
  GTCTTCAGGAGGAAACGGAGGCTTCACCTGGTGTTTGAATATTGTGACCACACAGTTCTCCATGAGTTG
  GACAGATACCAAAGAGGGGTACCAGAACATCTCGTGAAGAGCATAACTTGGCAGACACTGCAAGCTGTA
  AATTTTTGCCATAAACACAATTGCATACATAGAGACGTGAAGCCAGAAAATATCCTCATCACGAAACAT
  TCCGTGATTAAGCTTTGTGACTTTGGATTTGCTCGGCTTTTGACTGGACCGAGTGACTACTATACAGAC
  TACGTGGCTACCAGGTGGTACCGCTCCCCTGAGCTGCTGGTGGGGGACACGCAGTACGGCCCCCCGGTG
  GATGTTTGGGCAATTGGCTGTGTCTTTGCTGAGCTGCTGTCAGGAGTGCCTCTGTGGCCAGGAAAATCG
  GATGTGGATCAGCTGTATCTGATTAGGAAGACCTTGGGGGATCTCATTCCTAGGCACCAGCAAGTGTTT
  AGCACGAATCAGTACTTCAGTGGAGTGAAAATTCCAGACCCTGAAGATATGGAACCACTTGAATTAAAA
  TTCCCAAACATCTCTTATCCTGCCCTGGGGCTCCTAAAGGGCAGAGTACCAATTGCTTCAAGAACAGAA
  TAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001282236
• Insert Size: 831 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282236.1
• RefSeq Size: 2217 bp
• RefSeq ORF: 831 bp
• Locus ID: 8814
• Cytogenetics: 14q21.3
• Protein Families: Druggable Genome, Protein Kinase
• MW: 31.9 kDa
• Gene Summary: This gene product is a member of a large family of CDC2-related serine/threonine protein kinases that accumulates primarily in the nucleus. [provided by RefSeq, Nov 2018]
  Transcript Variant: This variant (2) differs in the 5' UTR, 3' UTR, and 3' coding sequence compared to variant 1. The resulting isoform (2) has a shorter and distinct C-terminus compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.