Acidic Calponin (CNN3) (NM_001286055) Human Untagged Clone

CAT#: SC335057

CNN3 (untagged) - Human calponin 3, acidic (CNN3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CNN3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335057 representing NM_001286055.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGACCCACTTCAACAAGGGCCCTTCCTATGGGCTCTCGGCCGAAGTCAAGAACAAGATTGCTTCCAAG
  TATGATCATCAGGCAGAAGAAGATCTTCGCAATTGGATAGAAGAGGTGACAGGCATGAGCATTGGCCCC
  AACTTCCAGCTGGGCTTAAAGGATGGCATCATCCTCTGCGAACTTATAAACAAGCTACAGCCAGGCTCA
  GTGAAGAAGGTCAACGAGTCCTCACTGAACTGGCCTCAGGCTAAAACAAAAGGATTCCATACAACCATT
  GACATTGGAGTTAAGTATGCAGAAAAACAAACAAGACGTTTTGATGAAGGAAAATTAAAAGCTGGCCAA
  AGTGTAATTGGTCTGCAGATGGGAACCAACAAATGTGCCAGCCAGGCAGGTATGACAGCTTACGGGACT
  AGGAGGCATCTTTATGATCCCAAAATGCAAACTGACAAACCTTTTGACCAGACCACAATTAGTCTGCAG
  ATGGGCACTAATAAAGGAGCCAGCCAGGCAGGGATGTTAGCACCAGGTACCAGAAGAGACATCTATGAT
  CAGAAGCTAACATTACAGCCGGTGGACAACTCGACAATTTCCCTACAGATGGGTACCAACAAAGTTGCT
  TCCCAGAAAGGAATGAGTGTGTATGGGCTTGGGCGGCAAGTATATGATCCCAAATACTGTGCTGCTCCT
  ACAGAACCTGTCATTCACAACGGAAGCCAAGGAACAGGAACAAATGGTTCGGAAATCAGTGATAGTGAT
  TATCAGGCAGAATACCCTGATGAGTATCATGGCGAGTACCAGGATGACTACCCCAGAGATTACCAATAT
  AGCGACCAAGGCATTGATTATTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001286055
• Insert Size: 852 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286055.1
• RefSeq Size: 1974 bp
• RefSeq ORF: 852 bp
• Locus ID: 1266
• UniProt ID: Q15417
• Cytogenetics: 1p21.3
• MW: 31.4 kDa
• Gene Summary: This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an in-frame exon in the central coding region compared to variant 1. The encoded isoform (2) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.