LMCD1 (NM_001278233) Human Untagged Clone

CAT#: SC335130

LMCD1 (untagged) - Human LIM and cysteine-rich domains 1 (LMCD1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LMCD1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335130 representing NM_001278233.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGACTCCAAGTATTCCACCCTCACTGCTCGGGTGAAAGGCGGGGACGGCATCCGGATTTACAAGAGG
  AACCGGATGATCATGACCAACCCTATTGCTACTGGGAAAGATCCCACTTTTGACACCATCACCTACGAG
  TGGGCTCCCCCTGGAGTCACCCAGAAACTGGGACTGCAGTACATGGAGCTCATCCCCAAGGAGAAGCAG
  CCAGTGACAGGCACAGAGGGTGCCTTTTACCGCCGCCGCCAGCTCATGCACCAGCTCCCCATCTATGAC
  CAGGATCCCTCGCGCTGCCGTGGACTTTTGGAGAATGAGTTGAAACTGATGGAAGAATTTGTCAAGCAA
  TATAAGAGCGAGGCCCTCGGCGTGGGAGAAGTGGCCCTCCCGGGGCAGGGTGGCTTGCCCAAGGAGGAG
  GGGAAGCAGCAGGAAAAGCCAGAGGGGGCAGAGACCACTGCTGCTACCACCAACGGCAGTCTCAGTGAC
  CCGTCCAAAGAAGTGGAATACGTCTGCGAGCTCTGCAAGGGAGCGGCCCCTCCTGACAGCCCCGTGGTC
  TACTCGGACAGGGCAGGCTACAACAAGCAGTGGCACCCCACCTGCTTTGTGTGTGCCAAGTGCTCCGAG
  CCGCTGGTGGACCTCATCTACTTCTGGAAGGATGGTGCACCCTGGTGCGGCCGCCATTACTGCGAGAGT
  CTGCGGCCCCGGTGCTCCGGCTGCGATGAGATAATATTCGCTGAGGACTACCAGCGTGTGGAAGATCTG
  GCCTGGCACCGAAAGCACTTTGTCTGTGAGGGTTGTGAGCAGCTGCTGAGCGGCCGGGCGTACATCGTC
  ACCAAGGGTCAGCTTCTGTGCCCAACTTGCAGCAAGTCCAAACGCTCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001278233
• Insert Size: 879 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278233.1
• RefSeq Size: 1668 bp
• RefSeq ORF: 879 bp
• Locus ID: 29995
• UniProt ID: Q9NZU5
• Cytogenetics: 3p25.3
• MW: 32.9 kDa
• Gene Summary: This gene encodes a member of the LIM-domain family of zinc finger proteins. The encoded protein contains an N-terminal cysteine-rich domain and two C-terminal LIM domains. The presence of LIM domains suggests involvement in protein-protein interactions. The protein may act as a co-regulator of transcription along with other transcription factors. Alternate splicing results in multiple transcript variants of this gene. [provided by RefSeq, May 2013]
  Transcript Variant: This variant (2) lacks an alternate exon in the 5' coding region and uses a downstream start codon compared to variant 1. The encoded isoform (2) is shorter at the N-terminus compared to isoform 1.