CD300 (CD300LF) (NM_001289085) Human Untagged Clone

CAT#: SC335145

CD300LF (untagged) - Human CD300 molecule-like family member f (CD300LF), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CD300LF
• Synonyms: CD300f; CLM-1; CLM1; IgSF13; IREM-1; IREM1; LMIR3; NKIR
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC335145 representing NM_001289085.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGCTGCCTCAGCTCGACCTCATGAGGGTCATCAGTGCTAAGAGTCAAGGCTACTCCATTGTCACT
  CAAATCACCGGTCCAACAACAGTGAATGGCTTGGAGCGGGGCTCCTTGACCGTGCAGTGTGTTTACAGA
  TCAGGCTGGGAGACCTACTTGAAGTGGTGGTGTCGAGGAGCTATTTGGCGTGACTGCAAGATCCTTGTT
  AAAACCAGTGGGTCAGAGCAGGAGGTGAAGAGGGACCGGGTGTCCATCAAGGACAATCAGAAAAACCGC
  ACGTTCACTGTGACCATGGAGGATCTCATGAAAACTGATGCTGACACTTACTGGTGTGGAATTGAGAAA
  ACTGGAAATGACCTTGGGGTCACAGTTCAAGTGACCATTGACCCAGCACCAGTCACCCAAGAAGAAACT
  AGCAGCTCCCCAACTCTGACCGGCCACCACTTGGACAACAGGCACAAGCTCCTGAAGCTCAGTGTCCTC
  CTGCCCCTCATCTTCACCATATTGCTGCTGCTTTTGGTGGCCGCCTCACTCTTGGCTTGGAGGATGATG
  AAGTACCAGCAGAAAGCAGCCGGGATGTCCCCAGAGCAGGTACTGCAGCCCCTGGAGGGCGACCTCTGC
  TATGCAGACCTGACCCTGCAGCTGGCCGGAACCTCCCCGCAAAAGGCTACCACGAAGCTTTCCTCTGCC
  CAGGTTGACCAGGTGGAAGTGGAATATGTCACCATGGCTTCCTTGCCGAAGGAGGACATTTCCTATGCA
  TCTCTGACCTTGGGTGCTGAGGATCAGGAACCGACCTACTGCAACATGGGCCACCTCAGTAGCCACCTC
  CCCGGCAGGGGCCCTGAGGAGCCCACGGAATACAGCACCATCAGCAGGCCTTAGTOPDB=YAWSCACHE=
  Y
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI Plasmid Map
• ACCN: NM_001289085
• Insert Size: 899 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289085.1
• RefSeq Size: 1984 bp
• RefSeq ORF: 899 bp
• Locus ID: 146722
• UniProt ID: Q8TDQ1
• Cytogenetics: 17q25.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 33.3 kDa
• Gene Summary: This gene encodes a member of the CD300 protein family. Members of this family are cell surface glycoproteins with a single IgV-like extracellular domain, and are involved in the regulation of immune response. The encoded protein is an inhibitory receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (5, also known as sv1) differs in its 5' UTR, and uses an alternate start codon, compared to variant 1. The encoded isoform (5) has a longer and distinct N-terminus, compared to isoform 1.