Synaptotagmin V (SYT5) (NM_001297774) Human Untagged Clone

CAT#: SC335762

SYT5 (untagged) - Human synaptotagmin V (SYT5), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SYT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001297774, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACTCGCGGTGGCTGTCCGGTTTCTGCGCGGCTGTTCGCGCAAAGGCCTCATGGGAGTTGGAGTTCTT
  TGTTGTGCGTGGGGCTCAAAGGACGTCTGGGGTGGGAATGGGGGACTAGGGTGCTTGAGTGTCTCTGCAG
  AAAAGACTCCAGGACCCCGCCACCATGTTCCCGGAGCCCCCAACCCCGGGGCCTCCATCGCCCGACACGC
  CTCCCGACTCCAGTCGCATCAGCCACGGCCCAGGTGCAGCCAGAAGTAGAGGAGCTGGAGCCAGCACCAT
  CCGGGCCAGGGCAGCAGGTGGCAGACAAGCATGAGCTAGGACGACTGCAGTACTCCCTGGATTATGACTT
  CCAGAGTGGCCAGCTGCTGGTGGGCATTCTGCAAGCAATGGGATTGGCAGCCTTGGATCTTGGTGGCTCC
  TCGGACCCCTATGTGCGGGTCTACCTGCTGCCGGACAAACGGAGGCGGTACGAGACCAAGGTGCATCGGC
  AGACGCTGAACCCTCACTTTGGGGAGACCTTCGCCTTCAAGGTCCCCTACGTGGAGCTGGGGGGCAGGGT
  GCTGGTCATGGCGGTGTACGACTTCGACCGCTTCTCTCGCAATGACGCCATCGGGGAGGTGCGGGTCCCT
  ATGAGCTCCGTGGACCTGGGGCGGCCAGTGCAGGCCTGGCGGGAGCTGCAGGCGGCTCCGCGGGAGGAGG
  AGAAGCTTGGGGACATCTGCTTCTCCCTCCGCTATGTCCCCACGGCCGGGAAGCTCACCGTCATCGTCCT
  GGAGGCTAAAAACCTGAAGAAGATGGACGTAGGAGGACTGTCAGATCCATACGTCAAGGTCCACCTGCTG
  CAGGGCGGCAAAAAGGTGCGGAAGAAGAAAACCACCATCAAGAAGAACACTCTGAACCCCTATTACAACG
  AAGCTTTCAGCTTCGAGGTGCCCTGTGACCAAGTCCAGAAGGTGCAGGTGGAGCTGACCGTGCTGGACTA
  CGACAAGCTGGGCAAGAACGAGGCCATCGGGAGGGTGGCCGTGGGGGCGGCCGCCGGCGGGGCTGGCCTG
  CGGCACTGGGCGGACATGCTGGCCAACCCGCGGCGGCCCATTGCCCAGTGGCACTCGCTGCGGCCCCCGG
  ACCGAGTGAGGCTGCTGCCTGCGCCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001297774
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001297774.1, NP_001284703.1
• RefSeq Size: 1797 bp
• RefSeq ORF: 1149 bp
• Locus ID: 6861
• Cytogenetics: 11p
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]'
  Transcript Variant: This variant (2) differs in the 5' UTR and contains multiple coding region differences, compared to variant 1. It encodes isoform 2, which is shorter and has a distinct N-terminus, compared to isoform 1.