GOT2 (NM_001286220) Human Untagged Clone

CAT#: SC335796

GOT2 (untagged) - Human glutamic-oxaloacetic transaminase 2, mitochondrial (GOT2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GOT2
• Synonyms: KAT4; KATIV; KYAT4; mitAAT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001286220, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCTGCTGCACTCCGGCCGCGTCCTCCCCGGGATCGCCGCCGCCTTCCACCCGGGCCTCGCCGCCG
  CGGCCTCTGCCAGAGCCAGCTCCTGGTGGACCCATGTGGAAATGGGACCTCCAGATCCCATTCTGGGAGT
  CACTGAAGCCTTTAAGAGGGACACCAATAGCAAAAAGATGAATCTGGGAGTTGGTGCCTACCGGGATGAT
  AATGGAAAGCCTTACGTTCTGCCTAGCGTCCGCAAGTTTGTCACTGTGCAGACCATTTCTGGAACTGGAG
  CCTTAAGGATCGGAGCCAGTTTTCTGCAAAGATTTTTTAAGTTCAGCCGAGATGTCTTTCTGCCCAAACC
  AACCTGGGGAAACCACACACCCATCTTCAGGGATGCTGGCATGCAGCTACAAGGTTATCGGTATTATGAC
  CCCAAGACTTGCGGTTTTGACTTCACAGGCGCTGTGGAGGATATTTCAAAAATACCAGAGCAGAGTGTTC
  TTCTTCTGCATGCCTGCGCCCACAATCCCACGGGAGTGGACCCGCGTCCGGAACAGTGGAAGGAAATAGC
  AACAGTGGTGAAGAAAAGGAATCTCTTTGCGTTCTTTGACATGGCCTACCAAGGCTTTGCCAGTGGTGAT
  GGTGATAAGGATGCCTGGGCTGTGCGCCACTTCATCGAACAGGGCATTAATGTTTGCCTCTGCCAATCAT
  ATGCCAAGAACATGGGCTTATATGGTGAGCGTGTAGGAGCCTTCACTATGGTCTGCAAAGATGCGGATGA
  AGCCAAAAGGGTAGAGTCACAGTTGAAGATCTTGATCCGTCCCATGTATTCCAACCCTCCCCTCAATGGG
  GCCCGGATTGCTGCTGCCATTCTGAACACCCCAGATTTGCGAAAACAATGGCTGCAAGAAGTGAAAGTCA
  TGGCTGACCGCATCATTGGCATGCGGACTCAACTGGTCTCCAACCTCAAGAAGGAGGGTTCCACCCACAA
  TTGGCAACACATCACCGACCAAATTGGCATGTTCTGTTTCACAGGGCTAAAGCCTGAACAGGTGGAGCGG
  CTGATCAAGGAGTTCTCCATCTACATGACAAAAGATGGCCGCATCTCTGTGGCAGGGGTCACCTCCAGCA
  ACGTGGGCTACCTTGCCCATGCCATTCACCAGGTCACCAAGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286220
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286220.1, NP_001273149.1
• RefSeq Size: 2359 bp
• RefSeq ORF: 1164 bp
• Locus ID: 2806
• Cytogenetics: 16q21
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Alanine, aspartate and glutamate metabolism, Arginine and proline metabolism, Cysteine and methionine metabolism, Metabolic pathways, Phenylalanine, tyrosine and tryptophan biosynthesis, Phenylalanine metabolism, Tyrosine metabolism
• Gene Summary: 'Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic and inner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and the urea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1.