GALK2 (NM_001289031) Human Untagged Clone

CAT#: SC336102

GALK2 (untagged) - Human galactokinase 2 (GALK2), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GALK2
• Synonyms: GK2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001289031, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTTTAACTCCAAGTTTGGATCTATTCCCAAGTTTTATGTTCGAGCACCAGGAAGAGTCAACATAATAG
  GAGAGCATATAGATTATTGTGGATATTCTGTTCTTCCTATGGCTGTAGAACAAGATGTGCTAATAGCTGT
  AGAACCTGTGAAAACGTACGCTCTCCAACTGGCCAATACAAATCCCTTGTATCCGGACTTCAGTACTAGT
  GCTAATAACATCCAGATTGATAAAACCAAGCCTTTGTGGCACAACTATTTCTTATGTGGACTTAAAGGAA
  TTCAGGAACACTTTGGTCTTAGTAACCTGACTGGAATGAACTGCCTGGTAGATGGAAATATCCCACCAAG
  TTCTGGCCTCTCCAGCTCCAGTGCTTTGGTCTGTTGTGCTGGCTTGGTGACGCTCACAGTGCTGGGAAGG
  AATCTATCCAAGGTGGAACTTGCAGAAATCTGTGCCAAGAGTGAGCGTTACATTGGCACTGAAGGAGGAG
  GCATGGACCAGTCTATATCATTTCTTGCAGAAGAAGGAACTGCCAAGTTGATAGAATTTAGTCCTCTGAG
  GGCAACCGATGTAAAACTCCCAAGTGGAGCAGTGTTTGTGATTGCCAACAGTTGTGTGGAGATGAATAAG
  GCAGCAACTTCCCATTTCAATATCAGGGTGATGGAGTGTCGGCTGGCTGCGAAGCTCCTGGCTAAATACA
  AAAGCTTGCAATGGGACAAAGTACTGAGGCTGGAGGAGGTGCAGGCTAAACTAGGGATTAGTCTAGAAGA
  AATGCTGTTGGTCACAGAAGATGCCCTTCATCCTGAACCCTATAACCCTGAGGAGATCTGCAGGTGTCTG
  GGAATTAGCCTGGAGGAACTCCGAACCCAAATCCTGAGTCCAAACACTCAAGATGTGCTCATCTTCAAAC
  TCTATCAGCGGGCAAAGCATGTGTACAGCGAGGCTGCGCGAGTGCTCCAGTTTAAGAAGATATGTGAAGA
  AGCACCTGAAAACATGGTCCAGCTGCTGGGAGAGTTGATGAACCAGAGCCACATGAGCTGCCGGGACATG
  TATGAGTGCAGCTGCCCCGAGCTGGATCAGCTGGTGGACATCTGTCGGAAGTTTGGGGCTCAAGGGTCAC
  GACTTACTGGAGCAGGATGGGGAGGCTGCACAGTATCAATGGTACCTGCGGACAAGCTGCCCAGCTTTCT
  AGCAAATGTGCACAAAGCTTATTACCAGAGGAGTGATGGAAGCTTAGCACCGGAGAAGCAAAGTTTGTTT
  GCTACCAAACCTGGAGGTGGGGCTTTGGTTTTGCTTGAGGCCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001289031
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001289031.1, NP_001275960.1
• RefSeq Size: 3313 bp
• RefSeq ORF: 1305 bp
• Locus ID: 2585
• Cytogenetics: 15q21.1-q21.2
• Protein Families: Druggable Genome
• Protein Pathways: Amino sugar and nucleotide sugar metabolism, Galactose metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes a highly efficient N-acetylgalactosamine (GalNAc) kinase, which has galactokinase activity when galactose is present at high concentrations. The encoded protein is a member of the GHMP kinase family. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2017]'
  Transcript Variant: This variant (4) uses an alternate splice site in the 5' coding region, and uses a downstream translation start codon, compared to variant 1. The encoded protein (isoform 3) has a shorter N-terminus, compared to isoform 1. Variants 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.