Complement factor 8 beta (C8B) (NM_001278544) Human Untagged Clone

CAT#: SC336631

C8B (untagged) - Human complement component 8, beta polypeptide (C8B), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: C8B
• Synonyms: C82
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001278544, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGACACTTGTATGACTCTGGCCTTCACTCTCTCTGGGCGTTTCTTCATGCTGCTTTCTCAGTACAGGT
  ATGCCTACTTGCTCCAGCCCTCTCAGTTCCATGGGGAACCGTGCAACTTCTCTGACAAGGAAGTCGAAGA
  CTGTGTTACCAACAGACCATGCAGAAGTCAAGTGCGATGTGAAGGCTTTGTGTGTGCACAGACAGGAAGG
  TGTGTAAACCGCAGACTTCTTTGCAATGGGGACAATGACTGTGGAGACCAGTCAGATGAAGCAAACTGTA
  GAAGGATTTATAAAAAATGTCAGCATGAAATGGACCAATACTGGGGAATTGGCAGTCTGGCCAGTGGGAT
  AAATTTGTTCACAAACAGTTTTGAGGGCCCAGTTCTTGATCACAGGTATTATGCAGGTGGATGCTCCCCG
  CATTACATCCTGAACACGAGGTTTAGGAAGCCCTACAATGTGGAAAGCTACACGCCACAGACCCAAGGCA
  AATACGAATTCATATTAAAAGAGTATGAATCATACTCAGATTTTGAACGCAATGTCACAGAGAAAATGGC
  AAGCAAGTCTGGTTTCAGTTTTGGTTTTAAAATACCTGGAATATTTGAACTTGGCATCAGTAGTCAAAGT
  GATCGAGGCAAACACTATATTAGGAGAACCAAACGATTCTCTCATACTAAAAGCGTATTTCTGCATGCAC
  GCTCTGACCTTGAAGTAGCACATTACAAGCTGAAACCCAGAAGCCTCATGCTCCATTACGAGTTCCTTCA
  GAGAGTTAAGCGGCTGCCCCTGGAGTACAGCTACGGGGAATACAGAGATCTCTTCCGTGATTTTGGGACC
  CACTACATCACAGAGGCTGTGCTTGGGGGCATTTATGAATACACCCTCGTTATGAACAAAGAGGCCATGG
  AGAGAGGAGATTATACTCTTAACAACGTCCATGCCTGTGCCAAAAATGATTTTAAAATTGGTGGTGCCAT
  TGAAGAGGTCTACGTCAGTCTGGGTGTGTCTGTAGGCAAATGCAGAGGTATTCTGAATGAAATAAAAGAC
  AGAAACAAGAGGGACACCATGGTGGAGGACTTGGTGGTCCTGGTACGAGGAGGGGCAAGTGAGCACATCA
  CCACCCTGGCATACCAGGAGCTGCCGACGGCGGACCTGATGCAGGAGTGGGGAGACGCTGTGCAGTACAA
  CCCAGCCATCATCAAAGTTAAGGTGGAGCCTCTGTATGAACTAGTGACAGCCACAGATTTTGCCTATTCC
  AGCACAGTGAGGCAGAACATGAAGCAGGCACTGGAGGAGTTCCAGAAGGAAGTTAGTTCCTGCCACTGTG
  CTCCCTGCCAAGGAAATGGAGTCCCTGTCCTGAAAGGATCACGCTGTGACTGCATCTGTCCTGTTGGATC
  CCAAGGCCTAGCCTGTGAGGTCTCCTATCGGAAGAATACCCCCATTGATGGGAAGTGGAATTGCTGGTCA
  AATTGGTCTTCATGCTCTGGAAGACGTAAGACAAGACAAAGGCAGTGTAACAATCCACCTCCTCAAAATG
  GGGGTAGCCCCTGTTCAGGCCCTGCTTCAGAAACACTTGACTGCTCCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278544
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278544.1, NP_001265473.1
• RefSeq Size: 2307 bp
• RefSeq ORF: 1590 bp
• Locus ID: 732
• Cytogenetics: 1p32.2
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Complement and coagulation cascades, Prion diseases, Systemic lupus erythematosus
• Gene Summary: 'This gene encodes one of the three subunits of the complement component 8 (C8) protein. C8 is composed of equimolar amounts of alpha, beta and gamma subunits, which are encoded by three separate genes. C8 is one component of the membrane attack complex, which mediates cell lysis, and it initiates membrane penetration of the complex. This protein mediates the interaction of C8 with the C5b-7 membrane attack complex precursor. In humans deficiency of this protein is associated with increased risk of meningococcal infections. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2013]'
  Transcript Variant: This variant (3) differs in the 5' UTR and 5' coding region compared to variant 1. These differences cause translation initiation at an alternate AUG and result in an isoform (3) with a shorter and distinct N-terminus, compared to isoform 1.