MCM7 (NM_001278595) Human Untagged Clone

CAT#: SC336710

MCM7 (untagged) - Human minichromosome maintenance complex component 7 (MCM7), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MCM7
• Synonyms: CDC47; MCM2; P1.1-MCM3; P1CDC47; P85MCM; PNAS146; PPP1R104
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001278595, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGTGGTGGCCACTTACACTTGTGACCAGTGTGGGGCAGAGACCTACCAGCCGATCCAGTCTCCCACTT
  TCATGCCTCTGATCATGTGCCCAAGCCAGGAGTGCCAAACCAACCGCTCAGGAGGGCGGCTGTATCTGCA
  GACACGGGGCTCCAGATTCATCAAATTCCAGGAGATGAAGATGCAAGAACATAGTGATCAGGTGCCTGTG
  GGAAATATCCCTCGTAGTATCACGGTGCTGGTAGAAGGAGAGAACACAAGGATTGCCCAGCCTGGAGACC
  ACGTCAGCGTCACTGGTATTTTCTTGCCAATCCTGCGCACTGGGTTCCGACAGGTGGTACAGGGTTTACT
  CTCAGAAACCTACCTGGAAGCCCATCGGATTGTGAAGATGAACAAGAGTGAGGATGATGAGTCTGGGGCT
  GGAGAGCTCACCAGGGAGGAGCTGAGGCAAATTGCAGAGGAGGATTTCTACGAAAAGCTGGCAGCTTCAA
  TCGCCCCAGAAATATACGGGCATGAAGATGTGAAGAAGGCACTGCTGCTCCTGCTAGTCGGGGGTGTGGA
  CCAGTCTCCTCGAGGCATGAAAATCCGGGGCAACATCAACATCTGTCTGATGGGGGATCCTGGTGTGGCC
  AAGTCTCAGCTCCTGTCATACATTGATCGACTGGCGCCTCGCAGCCAGTACACAACAGGCCGGGGCTCCT
  CAGGAGTGGGGCTTACGGCAGCTGTGCTGAGAGACTCCGTGAGTGGAGAACTGACCTTAGAGGGTGGGGC
  CCTGGTGCTGGCTGACCAGGGTGTGTGCTGCATTGATGAGTTCGACAAGATGGCTGAGGCCGACCGCACA
  GCCATCCACGAGGTCATGGAGCAGCAGACCATCTCCATTGCCAAGGCCGGCATTCTCACCACACTCAATG
  CCCGCTGCTCCATCCTGGCTGCCGCCAACCCTGCCTACGGGCGCTACAACCCTCGCCGCAGCCTGGAGCA
  GAACATACAGCTACCTGCTGCACTGCTCTCCCGGTTTGACCTCCTCTGGCTGATTCAGGACCGGCCCGAC
  CGAGACAATGACCTACGGTTGGCCCAGCACATCACCTATGTGCACCAGCACAGCCGGCAGCCCCCCTCCC
  AGTTTGAACCTCTGGACATGAAGCTCATGAGGCGTTACATAGCCATGTGCCGCGAGAAGCAGCCCATGGT
  GCCAGAGTCTCTGGCTGACTACATCACAGCAGCATACGTGGAGATGAGGCGAGAGGCTTGGGCTAGTAAG
  GATGCCACCTATACTTCTGCCCGGACCCTGCTGGCTATCCTGCGCCTTTCCACTGCTCTGGCACGTCTGA
  GAATGGTGGATGTGGTGGAGAAAGAAGATGTGAATGAAGCCATCAGGCTAATGGAGATGTCAAAGGACTC
  TCTTCTAGGAGACAAGGGGCAGACAGCTAGGACTCAGAGACCAGCAGATGTGATATTTGCCACCGTCCGT
  GAACTGGTCTCAGGGGGCCGAAGTGTCCGGTTCTCTGAGGCAGAGCAGCGCTGTGTATCTCGTGGCTTCA
  CACCCGCCCAGTTCCAGGCGGCTCTGGATGAATATGAGGAGCTCAATGTCTGGCAGGTCAATGCTTCCCG
  GACACGGATCACTTTTGTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278595
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278595.1, NP_001265524.1
• RefSeq Size: 3105 bp
• RefSeq ORF: 1632 bp
• Locus ID: 4176
• Cytogenetics: 7q22.1
• Protein Families: Transcription Factors
• Protein Pathways: Cell cycle, DNA replication
• Gene Summary: 'The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are essential for the initiation of eukaryotic genome replication. The hexameric protein complex formed by the MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. The MCM complex consisting of this protein and MCM2, 4 and 6 proteins possesses DNA helicase activity, and may act as a DNA unwinding enzyme. Cyclin D1-dependent kinase, CDK4, is found to associate with this protein, and may regulate the binding of this protein with the tumorsuppressor protein RB1/RB. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) contains an additional internal segment, which results in translation initiation from an in-frame downstream AUG compared to variant 1. The resulting isoform (2) has a shorter N-terminus compared to isoform 1. Variants 2 and 3 encode the same isoform.