CDC25C (NM_001287583) Human Untagged Clone

CAT#: SC336756

CDC25C (untagged) - Human cell division cycle 25C (CDC25C), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC25C
• Synonyms: CDC25; PPP1R60
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001287583, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGGGCGCAGCTCCGTGTCGTGTCTCTCTCAAAGTGTCTCTCGGACAGCGCTGTTGGGAACGAGCCT
  GGCGCTTTGATTTAGGGATGGGGGTGGTTTCCTCACTAACGAGTCACCCCCTCCAAAATATCGCAGAAAG
  TGGACGGCCCCAGTTGGAGGCACATCTTGAAACCCGCGGGGGAGGCCATGTCGGAAGAAGGTTTTGTTTT
  TCTCCTGGTGAGAATTCGAAGACCATGTCTACGGAACTCTTCTCATCCACAAGAGAGGAAGGAAGCTCTG
  GCTCAGGACCCAGTTTTAGGTCTAATCAAAGGAAAATGTTAAACCTGCTCCTGGAGAGAGACACTTCCTT
  TACCGTCTGTCCAGATGTCCCTAGAACTCCAGTGGGCAAATTTCTTGGTGATTCTGCAAACCTAAGCATT
  TTGTCTGGAGGAACCCCAAAACGTTGCCTCGATCTTTCGAATCTTAGCAGTGGGGAGATAACTGCCACTC
  AGCTTACCACTTCTGCAGACCTTGATGAAACTGGTCACCTGGATTCTTCAGGACTTCAGGAAGTGCATTT
  AGCTGGGATGAATCATGACCAGCACCTAATGAAATGTAGCCCAGCACAGCTTCTTTGTAGCACTCCGAAT
  GGTTTGGACCGTGGCCATAGAAAGAGAGATGCAATGTGTAGTTCATCTGCAAATAAAGAAAATGACAATG
  GAAACTTGGTGGACAGTGAAATGAAATATTTGGGCAGTCCCATTACTACTGTTCCAAAATTGGATAAAAA
  TCCAAACCTAGGAGAAGACCAGGCAGAAGAGATTTCAGATGAATTAATGGAGTTTTCCCTGAAAGATCAA
  GAAGCAAAGGTGAGCAGAAGTGGCCTATATCGCTCCCCGTCGATGCCAGAGAACTTGAACAGGCCAAGAC
  TGAAGCAGGTGGAAAAATTCAAGGACAACACAATACCAGATAAAGTTAAAAAAAAGTATTTTTCTGGCCA
  AGGAAAGCTCAGGAAGGGCTTATGTTTAAAGAAGACAGTCTCTCTGTGTGACATTACTATCACTCAGATG
  CTGGAGGAAGATTCTAACCAGGGGCACCTGATTGGTGATTTTTCCAAGGTATGTGCGCTGCCAACCGTGT
  CAGGGAAACACCAAGATCTGAAGTATGTCAACCCAGAAACAGTGGCTGCCTTACTGTCGGGGAAGTTCCA
  GGGTCTGATTGAGAAGTTTTATGTCATTGATTGTCGCTATCCATATGAGTATCTGGGAGGACACATCCAG
  GGAGCCTTAAACTTATATAGTCAGGAAGAACTGTTTAACTTCTTTCTGAAGAAGCCCATCGTCCCTTTGG
  ACACCCAGAAGAGAATAATCATCGTGTTCCACTGTGAATTCTCCTCAGAGAGGGGCCCCCGAATGTGCCG
  CTGTCTGCGTGAAGAGGACAGGTCTCTGAACCAGTATCCTGCATTGTACTACCCAGAGCTATATATCCTT
  AAAGGCGGCTACAGAGACTTCTTTCCAGAATATATGGAACTGTGTGAACCACAGAGCTACTGCCCTATGC
  ATCATCAGGACCACAAGACTGAGTTGCTGAGGTGTCGAAGCCAGAGCAAAGTGCAGGAAGGGGAGCGGCA
  GCTGCGGGAGCAGATTGCCCTTCTGGTGAAGGACATGAGCCCATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001287583
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001287583.1, NP_001274512.1
• RefSeq Size: 2304 bp
• RefSeq ORF: 1656 bp
• Locus ID: 995
• Cytogenetics: 5q31.2
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, Oocyte meiosis, Progesterone-mediated oocyte maturation
• Gene Summary: 'This gene encodes a conserved protein that plays a key role in the regulation of cell division. The encoded protein directs dephosphorylation of cyclin B-bound CDC2 and triggers entry into mitosis. It also suppresses p53-induced growth arrest. Multiple alternatively spliced transcript variants of this gene have been described. [provided by RefSeq, Dec 2015]'
  Transcript Variant: This variant (4) differs in the 5' UTR, uses an alternate splice site in the 5' region, and initiates translation at an alternate start codon compared to variant 1. The encoded isoform (c) has a longer N-terminus than isoform a.