ETFDH (NM_001281738) Human Untagged Clone

CAT#: SC336777

ETFDH (untagged) - Human electron-transferring-flavoprotein dehydrogenase (ETFDH), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ETFDH
• Synonyms: ETFQO; MADD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001281738, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAAGGTTTGCAGAAGAAGCAGATGTTGTAATAGTTGGTGCAGGCCCTGCAGGGCTCTCTGCAGCTG
  TTCGTCTAAAACAGTTGGCTGTGGCACATGAAAAGGACATCCGTGTGTGTCTAGTGGAGAAAGCTGCCCA
  GATAGGAGCTCATACTCTCTCAGGGGCTTGCCTTGATCCAGGTGCTTTTAAAGAACTCTTCCCAGACTGG
  AAAGAGAAGGGGGCTCCACTTAACACTCCTGTAACAGAAGACAGATTTGGAATTTTAACAGAGAAATACA
  GAATTCCTGTGCCAATTCTTCCAGGGCTTCCAATGAATAATCATGGCAATTACATTGTACGCTTGGGACA
  TTTAGTGAGCTGGATGGGCGAACAAGCAGAAGCCCTTGGTGTTGAAGTATACCCTGGTTATGCAGCTGCT
  GAGGTCCTTTTTCATGATGATGGTAGTGTAAAAGGAATTGCCACTAACGATGTAGGGATACAAAAGGATG
  GTGCACCAAAGGCAACATTTGAGAGAGGACTGGAACTACATGCTAAAGTCACAATTTTTGCAGAAGGTTG
  CCATGGACATCTAGCCAAGCAACTATATAAGAAGTTTGATTTGAGAGCAAATTGTGAACCTCAAACCTAC
  GGGATTGGACTGAAGGAGTTATGGGTTATTGATGAAAAGAACTGGAAACCTGGGAGAGTAGATCACACTG
  TTGGTTGGCCCTTGGACAGACATACCTATGGAGGATCTTTCCTCTATCATTTGAATGAAGGTGAACCCCT
  AGTAGCTCTTGGTCTTGTGGTTGGTCTAGACTATCAGAATCCATACCTGAGTCCATTTAGAGAGTTCCAA
  AGGTGGAAACACCATCCTAGCATTCGGCCAACCTTGGAAGGTGGAAAAAGGATTGCATACGGAGCCAGAG
  CTCTCAATGAAGGTGGCTTTCAGTCTATACCAAAACTCACCTTTCCTGGTGGTTTACTAATTGGTTGTAG
  TCCTGGTTTTATGAATGTTCCCAAGATCAAAGGTACTCACACAGCAATGAAAAGTGGAATTTTAGCAGCA
  GAATCTATTTTTAATCAACTAACTAGTGAAAATCTCCAATCAAAGACAATAGGACTCCATGTAACTGAAT
  ATGAGGACAATTTGAAGAACTCATGGGTATGGAAAGAGCTATATTCTGTTAGAAATATAAGACCGTCCTG
  CCACGGAGTACTGGGTGTATATGGAGGGATGATTTACACTGGAATCTTTTACTGGATATTGAGAGGAATG
  GAGCCGTGGACTCTGAAACATAAAGGTTCTGACTTTGAACGGCTCAAGCCAGCCAAGGATTGCACACCTA
  TTGAGTATCCAAAACCCGATGGACAGATCAGTTTTGACCTCTTGTCATCTGTGGCTCTGAGTGGTACTAA
  TCATGAACATGACCAGCCGGCACACTTAACCTTAAGGGATGACAGTATACCTGTAAATAGAAATCTGTCG
  ATATATGATGGGCCCGAGCAGCGATTCTGTCCTGCAGGAGTTTATGAATTTGTACCTGTGGAACAAGGTG
  ATGGATTTCGGTTACAGATAAATGCTCAGAACTGTGTACATTGTAAAACATGTGATATTAAAGATCCAAG
  TCAGAATATTAACTGGGTGGTACCTGAAGGTGGAGGAGGACCTGCTTACAATGGAATGTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001281738
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001281738.1, NP_001268667.1
• RefSeq Size: 1952 bp
• RefSeq ORF: 1671 bp
• Locus ID: 2110
• Cytogenetics: 4q32.1
• Gene Summary: 'This gene encodes a component of the electron-transfer system in mitochondria and is essential for electron transfer from a number of mitochondrial flavin-containing dehydrogenases to the main respiratory chain. Mutations in this gene are associated with glutaric acidemia. Alternatively spliced transcript variants that encode distinct isoforms have been observed. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (3) represents use of an alternate promoter, differs in the 5' UTR, and uses a downstream start codon compared to variant 1. The resulting protein (isoform 3) is shorter and has a distinct N-terminus compared to isoform 1.