STT3A (NM_001278504) Human Untagged Clone

CAT#: SC336981

STT3A (untagged) - Human STT3A, subunit of the oligosaccharyltransferase complex (catalytic) (STT3A), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: STT3A
• Synonyms: ITM1; STT3-A; TMC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001278504, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGATCACCTCTGCTGCAATCTACCATGTACTCCATTTTTTCCACATCACCATCGACATTCGGAATGTCT
  GTGTGTTCCTGGCCCCTCTCTTCTCCTCCTTCACCACCATCGTCACGTACCACCTTACCAAAGAGCTCAA
  GGATGCAGGGGCTGGGCTTCTTGCTGCTGCCATGATTGCTGTAGTTCCTGGATATATCTCCCGATCTGTG
  GCTGGCTCCTATGATAATGAAGGGATTGCCATCTTTTGCATGCTACTCACCTACTACATGTGGATCAAGG
  CAGTAAAGACTGGTTCCATCTGTTGGGCAGCTAAGTGTGCCCTTGCTTATTTCTACATGGTCTCGTCATG
  GGGAGGTTATGTGTTCCTGATCAACTTAATTCCTCTCCACGTCCTCGTGCTGATGCTCACAGGCCGTTTC
  TCTCACCGGATCTATGTGGCCTACTGTACTGTTTACTGCCTGGGCACTATACTTTCTATGCAGATCTCCT
  TTGTGGGTTTCCAGCCTGTCCTTTCATCAGAGCACATGGCAGCCTTTGGGGTCTTTGGTCTCTGCCAGAT
  CCATGCCTTTGTGGATTACCTGCGCAGCAAGTTGAATCCACAACAATTTGAAGTTCTTTTCCGGAGCGTC
  ATCTCTCTGGTAGGCTTTGTCCTTCTCACCGTGGGAGCTCTCCTCATGCTGACAGGAAAAATATCTCCCT
  GGACGGGGCGTTTCTACTCGCTGCTGGATCCCTCTTATGCTAAGAACAACATCCCCATCATTGCTTCTGT
  GTCTGAGCATCAGCCCACAACCTGGTCCTCATACTATTTTGACCTGCAGCTCCTCGTCTTCATGTTTCCA
  GTTGGCCTCTATTACTGCTTTAGCAACCTGTCTGATGCCCGGATTTTTATCATCATGTATGGTGTGACCA
  GCATGTACTTTTCAGCTGTAATGGTGCGTCTAATGCTAGTGTTGGCACCTGTTATGTGCATTCTCTCTGG
  CATTGGAGTCTCCCAGGTGCTGTCCACATACATGAAGAATCTGGACATAAGTCGTCCAGACAAGAAGAGC
  AAGAAGCAACAGGATTCCACCTACCCTATTAAGAATGAAGTGGCAAGTGGGATGATACTGGTCATGGCTT
  TCTTTCTCATCACCTACACCTTTCATTCAACCTGGGTGACCAGTGAGGCCTACTCTTCTCCGTCCATTGT
  ACTATCTGCCCGTGGTGGGGATGGCAGTAGGATCATATTTGATGACTTCCGAGAAGCATATTATTGGCTT
  CGTCATAATACTCCAGAGGATGCGAAGGTCATGTCCTGGTGGGATTATGGCTATCAGATTACAGCTATGG
  CAAACCGAACAATTTTAGTGGACAATAACACATGGAATAATACCCATATTTCTCGAGTAGGGCAGGCAAT
  GGCGTCCACAGAGGAAAAAGCCTATGAGATCATGAGGGAGCTCGATGTCAGCTATGTGCTGGTCATTTTT
  GGAGGCCTCACTGGGTATTCCTCTGATGATATCAACAAGTTTCTTTGGATGGTCCGGATTGGAGGGAGCA
  CAGATACAGGCAAACATATCAAGGAGAATGACTATTATACTCCAACTGGGGAGTTCCGTGTGGACCGTGA
  AGGTTCTCCAGTGCTGCTCAACTGCCTCATGTACAAGATGTGTTACTATCGCTTTGGACAGGTTTACACA
  GAAGCCAAGCGTCCTCCAGGCTTTGACCGTGTCCGAAATGCTGAGATTGGGAATAAAGACTTTGAGCTTG
  ATGTCCTGGAGGAAGCATATACCACAGAACATTGGCTGGTCAGGATATACAAGGTAAAGGACCTGGATAA
  TCGAGGCTTGTCAAGGACATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278504
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278504.1, NP_001265433.1
• RefSeq Size: 4121 bp
• RefSeq ORF: 1842 bp
• Locus ID: 3703
• Cytogenetics: 11q24.2
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• Gene Summary: 'The protein encoded by this gene is a catalytic subunit of the N-oligosaccharyltransferase (OST) complex, which functions in the endoplasmic reticulum to transfer glycan chains to asparagine residues of target proteins. A separate complex containing a similar catalytic subunit with an overlapping function also exists. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2015]'
  Transcript Variant: This variant (3) differs in the 5' UTR and lacks the exon containing the translation start codon compared to variant 1. These differences cause translation initiation at a downstream AUG and result in an isoform (b) with a shorter N-terminus, compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.