HIF1 beta (ARNT) (NM_001286035) Human Untagged Clone

CAT#: SC337433

ARNT (untagged) - Human aryl hydrocarbon receptor nuclear translocator (ARNT), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ARNT
• Synonyms: bHLHe2; HIF-1-beta; HIF-1beta; HIF1-beta; HIF1B; HIF1BETA; TANGO
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001286035, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACATCAGATGTACCATCACTGGGTCCAGCCATTGCCTCTGGAAACTCTGGACCTGGAATTCAAGGTG
  GAGGAGCCATTGTCCAGAGGGCTATTAAGCGGCGACCAGGGCTGGATTTTGATGATGATGGAGAAGGGAA
  CAGTAAATTTTTGAGGTGTGATGATGATCAGATGTCTAACGATAAGGAGCGGTTTGCCAGGTCGGATGAT
  GAGCAGAGCTCTGCGGATAAAGAGAGACTTGCCAGGGAAAATCACAGTGAAATTGAACGGCGGCGACGGA
  ACAAGATGACAGCCTACATCACAGAACTGTCAGATATGGTACCCACCTGTAGTGCCCTGGCTCGAAAACC
  AGACAAGCTAACCATCTTACGCATGGCAGTTTCTCACATGAAGTCCTTGCGGGGAACTGGCAACACATCC
  ACTGATGGCTCCTATAAGCCGTCTTTCCTCACTGATCAGGAACTGAAACATTTGATCTTGGAGGCAGCAG
  ATGGCTTTCTGTTTATTGTCTCATGTGAGACAGGCAGGGTGGTGTATGTGTCTGACTCCGTGACTCCTGT
  TTTGAACCAGCCACAGTCTGAATGGTTTGGCAGCACACTCTATGATCAGGTGCACCCAGATGATGTGGAT
  AAACTTCGTGAGCAGCTTTCCACTTCAGAAAATGCCCTGACAGGGCGTATCCTGGATCTAAAGACTGGAA
  CAGTGAAAAAGGAAGGTCAGCAGTCTTCCATGAGAATGTGTATGGGCTCAAGGAGATCGTTTATTTGCCG
  AATGAGGTGTGGCAGTAGCTCTGTGGACCCAGTTTCTGTGAATAGGCTGAGCTTTGTGAGGAACAGATGC
  AGGAATGGACTTGGCTCTGTAAAGGATGGGGAACCTCACTTCGTGGTGGTCCACTGCACAGGCTACATCA
  AGGCCTGGCCCCCAGCAGATGATGACCCAGAGGCTGGCCAGGGAAGCAAGTTTTGCCTAGTGGCCATTGG
  CAGATTGCAGGTAACTAGTTCTCCCAACTGTACAGACATGAGTAATGTTTGTCAACCAACAGAGTTCATC
  TCCCGACACAACATTGAGGGTATCTTCACTTTTGTGGATCACCGCTGTGTGGCTACTGTTGGCTACCAGC
  CACAGGAACTCTTAGGAAAGAATATTGTAGAATTCTGTCATCCTGAAGACCAGCAGCTTCTAAGAGACAG
  CTTCCAACAGGTAGTGAAATTAAAAGGCCAAGTGCTGTCTGTCATGTTCCGGTTCCGGTCTAAGAACCAA
  GAATGGCTCTGGATGAGAACCAGCTCCTTTACTTTCCAGAACCCTTACTCAGATGAAATTGAGTACATCA
  TCTGTACCAACACCAATGTGAAGAACTCTAGCCAAGAACCACGGCCTACACTCTCCAACACAATCCAGAG
  GCCACAACTAGGTCCCACAGCTAATTTACCCCTGGAGATGGGCTCAGGACAGCTGGCACCCAGGCAGCAG
  CAACAGCAAACAGAATTGGACATGGTACCAGGAAGAGATGGACTGGCCAGCTACAATCATTCCCAGGTGG
  TTCAGCCTGTGACAACCACAGGACCAGAACACAGCAAGCCCCTTGAGAAGTCAGATGGTTTATTTGCCCA
  GGATAGAGATCCAAGATTTTCAGAAATCTATCACAACATCAATGCGGATCAGAGTAAAGGCATCTCCTCC
  AGCACTGTCCCTGCCACCCAACAGCTATTCTCCCAGGGCAACACATTCCCTCCTACCCCCCGGCCGGCAG
  AGAATTTCAGGAATAGTGGCCTAGCCCCTCCTGTAACCATTGTCCAGCCATCAGCTTCTGCAGGACAGAT
  GTTGGCCCAGATTTCCCGCCACTCCAACCCCACCCAAGGAGCAACCCCAACTTGGACCCCTACTACCCGC
  TCAGGCTTTTCTGCCCAGCAGGTGGCTACCCAGGCTACTGCTAAGACTCGTACTTCCCAGTTTGGTGTGG
  GCAGCTTTCAGACTCCATCCTCCTTCAGCTCCATGTCCCTCCCTGGTGCCCCAACTGCATCGCCTGGTGC
  TGCTGCCTACCCTAGTCTCACCAATCGTGGATCTAACTTTGCTCCTGAGACTGGACAGACTGCAGGACAA
  TTCCAGACACGGACAGCAGAGGGTGTGGGTGTCTGGCCACAGTGGCAGGGCCAGCAGCCTCATCATCGTT
  CAAGTTCTAGTGAGCAACATGTTCAACAACCGCCAGCACAGCAACCTGGCCAGCCTGAGGTCTTCCAGGA
  GATGCTGTCCATGCTGGGAGATCAGAGCAACAGCTACAACAATGAAGAATTCCCTGATCTAACTATGTTT
  CCCCCCTTTTCAGAATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286035
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286035.1, NP_001272964.1
• RefSeq Size: 4944 bp
• RefSeq ORF: 2328 bp
• Locus ID: 405
• Cytogenetics: 1q21.3
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Pathways in cancer, Renal cell carcinoma
• Gene Summary: 'This gene encodes a protein containing a basic helix-loop-helix domain and two characteristic PAS domains along with a PAC domain. The encoded protein binds to ligand-bound aryl hydrocarbon receptor and aids in the movement of this complex to the nucleus, where it promotes the expression of genes involved in xenobiotic metabolism. This protein is also a co-factor for transcriptional regulation by hypoxia-inducible factor 1. Chromosomal translocation of this locus with the ETV6 (ets variant 6) gene on chromosome 12 have been described in leukemias. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2013]'
  Transcript Variant: This variant (5) contains an alternate exon in the 5' region, initiates translation at a downstream in-frame start codon, and uses an alternate in-frame splice site, compared to variant 1. The encoded isoform (5) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.