Aconitase 1 (ACO1) (NM_001278352) Human Untagged Clone

CAT#: SC337635

ACO1 (untagged) - Human aconitase 1, soluble (ACO1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACO1
• Synonyms: ACONS; HEL60; IREB1; IREBP; IREBP1; IRP1
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001278352, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGCAACCCATTCGCACACCTTGCTGAGCCATTGGATCCTGTACAACCAGGAAAGAAATTCTTCAATT
  TGAATAAATTGGAGGATTCAAGATATGGGCGCTTACCATTTTCGATCAGAGTTCTTCTGGAAGCAGCCAT
  TCGGAATTGTGATGAGTTTTTGGTGAAGAAACAGGATATTGAAAATATTCTACATTGGAATGTCACGCAG
  CACAAGAACATAGAAGTGCCATTTAAGCCTGCTCGTGTCATCCTGCAGGACTTTACGGGTGTGCCCGCTG
  TGGTTGACTTTGCTGCAATGCGTGATGCTGTGAAAAAGTTAGGAGGAGATCCAGAGAAAATAAACCCTGT
  CTGCCCTGCTGATCTTGTAATAGATCATTCCATCCAGGTTGATTTCAACAGAAGGGCAGACAGTTTACAG
  AAGAATCAAGACCTGGAATTTGAAAGAAATAGAGAGCGATTTGAATTTTTAAAGTGGGGTTCCCAGGCTT
  TTCACAACATGCGGATTATTCCCCCTGGCTCAGGAATCATCCACCAGGTGAATTTGGAATATTTGGCAAG
  AGTGGTATTTGATCAGGATGGATATTATTACCCAGACAGCCTCGTGGGCACAGACTCGCACACTACCATG
  ATTGATGGCTTGGGCATTCTTGGTTGGGGTGTCGGTGGTATTGAAGCAGAAGCTGTCATGCTGGGTCAGC
  CAATCAGTATGGTGCTTCCTCAGGTGATTGGCTACAGGCTGATGGGGAAGCCCCACCCTCTGGTAACATC
  CACTGACATCGTGCTCACCATTACCAAGCACCTCCGCCAGGTTGGGGTAGTGGGCAAATTTGTCGAGTTC
  TTCGGGCCTGGAGTAGCCCAGTTGTCCATTGCTGACCGAGCTACGATTGCTAACATGTGTCCAGAGTACG
  GAGCAACTGCTGCCTTTTTCCCAGTTGATGAAGTTAGTATCACGTACCTGGTGCAAACAGGTCGTGATGA
  AGAAAAATTAAAGTATATTAAAAAATATCTTCAGGCTGTAGGAATGTTTCGAGATTTCAATGACCCTTCT
  CAAGACCCAGACTTCACCCAGGTTGTGGAATTAGATTTGAAAACAGTAGTGCCTTGCTGTAGTGGACCCA
  AAAGGCCTCAGGACAAAGTTGCTGTGTCCGACATGAAAAAGGACTTTGAGAGCTGCCTTGGAGCCAAGCA
  AGGATTTAAAGGATTCCAAGTTGCTCCTGAACATCATAATGACCATAAGACCTTTATCTATGATAACACT
  GAATTCACCCTTGCTCATGGTTCTGTGGTCATTGCTGCCATTACTAGCTGCACAAACACCAGTAATCCGT
  CTGTGATGTTAGGGGCAGGATTGTTAGCAAAGAAAGCTGTGGATGCTGGCCTGAACGTGATGCCTTACAT
  CAAAACTAGCCTGTCTCCTGGGAGTGGCGTGGTCACCTACTACCTACAAGAAAGCGGAGTCATGCCTTAT
  CTGTCTCAGCTTGGGTTTGACGTGGTGGGCTATGGCTGCATGACCTGCATTGGCAACAGTGGGCCTTTAC
  CTGAACCTGTGGTAGAAGCCATCACACAGGGAGACCTTGTAGCTGTTGGAGTACTATCTGGAAACAGGAA
  TTTTGAAGGTCGAGTTCACCCCAACACCCGGGCCAACTATTTAGCCTCTCCCCCCTTAGTAATAGCATAT
  GCAATTGCTGGAACCATCAGAATCGACTTTGAGAAAGAGCCATTGGGAGTAAATGCAAAGGGACAGCAGG
  TATTTCTGAAAGATATCTGGCCGACTAGAGACGAGATCCAGGCAGTGGAGCGTCAGTATGTCATCCCGGG
  GATGTTTAAGGAAGTCTATCAGAAAATAGAGACTGTGAATGAAAGCTGGAATGCCTTAGCAACCCCATCA
  GATAAGCTGTTTTTCTGGAATTCCAAATCTACGTATATCAAATCACCACCATTCTTTGAAAACCTGACTT
  TGGATCTTCAGCCCCCTAAATCTATAGTGGATGCCTATGTGCTGCTAAATTTGGGAGATTCGGTAACAAC
  TGACCACATCTCCCCAGCTGGAAATATTGCAAGAAACAGTCCTGCTGCTCGCTACTTAACTAACAGAGGC
  CTAACTCCACGAGAATTCAACTCCTATGGCTCCCGCCGAGGTAATGACGCCGTCATGGCACGGGGAACAT
  TTGCCAACATTCGCTTGTTAAACAGATTTTTGAACAAGCAGGCACCACAGACTATCCATCTGCCTTCTGG
  GGAAATCCTTGATGTGTTTGATGCTGCTGAGCGGTACCAGCAGGCAGGCCTTCCCCTGATCGTTCTGGCT
  GGCAAAGAGTACGGTGCAGGCAGCTCCCGAGACTGGGCAGCTAAGGGCCCTTTCCTGCTGGGAATCAAAG
  CCGTCCTGGCCGAGAGCTACGAGCGCATTCACCGCAGTAACCTGGTTGGGATGGGTGTGATCCCACTTGA
  ATATCTCCCTGGTGAGAATGCAGATGCCCTGGGGCTCACAGGGCAAGAACGATACACTATCATTATTCCA
  GAAAACCTCAAACCACAAATGAAAGTCCAGGTCAAGCTGGATACTGGCAAGACCTTCCAGGCTGTCATGA
  GGTTTGACACTGATGTGGAGCTCACTTATTTCCTCAACGGGGGCATCCTCAACTACATGATCCGCAAGAT
  GGCCAAGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001278352
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001278352.1, NP_001265281.1
• RefSeq Size: 3633 bp
• RefSeq ORF: 2670 bp
• Locus ID: 48
• Cytogenetics: 9p21.1
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Glyoxylate and dicarboxylate metabolism, Metabolic pathways
• Gene Summary: 'The protein encoded by this gene is a bifunctional, cytosolic protein that functions as an essential enzyme in the TCA cycle and interacts with mRNA to control the levels of iron inside cells. When cellular iron levels are high, this protein binds to a 4Fe-4S cluster and functions as an aconitase. Aconitases are iron-sulfur proteins that function to catalyze the conversion of citrate to isocitrate. When cellular iron levels are low, the protein binds to iron-responsive elements (IREs), which are stem-loop structures found in the 5' UTR of ferritin mRNA, and in the 3' UTR of transferrin receptor mRNA. When the protein binds to IRE, it results in repression of translation of ferritin mRNA, and inhibition of degradation of the otherwise rapidly degraded transferrin receptor mRNA. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Alternative splicing results in multiple transcript variants [provided by RefSeq, Jan 2014]'
  Transcript Variant: This variant (1) represents the longer transcript. Variants 1, 2 and 3 encode the same protein.