GRID2 (NM_001286838) Human Untagged Clone

CAT#: SC337665

GRID2 (untagged) - Human glutamate receptor, ionotropic, delta 2 (GRID2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRID2
• Synonyms: GluD2; SCAR18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001286838, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAAGTTTTCCCCTTTCTCTTGGTTTTGTCCGTCTGGTGGTCTCGAACCTGGGACTCGGCGAATGCGG
  ATTCGATCATTCACATCGGAGCAATTTTTGATGAATCTGCCAAAAAGGATGATGAGGTATTTCGCACTGC
  GGTTGGTGACCTTAACCAGAATGAGGAGATCTTACAGACTGAGAAAATCACATTTTCAGTGACGTTTGTT
  GATGGCAACAACCCTTTCCAAGCAGTTCAAGAAGATATCCGTGGAATACAGGAGTTCTTGGACAAAGTCT
  CTCAGCAGGGAATGGATGTTGCACTTCAGAAGGTAGAAAACAACATCAATAAAATGATTACCACTCTCTT
  TGACACCATGAGAATAGAAGAACTGAATCGCTATCGAGACACTCTTAGGCGAGCGATCCTTGTTATGAAT
  CCTGCTACAGCCAAATCCTTCATTACTGAGGTTGTGGAGACTAATTTGGTTGCTTTTGACTGTCACTGGA
  TCATTATAAATGAGGAAATAAACGATGTGGACGTACAGGAACTTGTAAGAAGGTCAATTGGAAGGTTAAC
  GATTATTCGGCAGACATTTCCAGTTCCCCAGAACATAAGTCAGCGGTGTTTCCGTGGCAACCATCGAATA
  TCTTCAACATTGTGTGATCCAAAGGATCCATTTGCTCAGAATATGGAGATTTCCAACCTTTACATATATG
  ACACGGTGCTTCTGCTTGCTAATGCTTTTCATAAGAAGCTGGAGGACCGAAAGTGGCACAGCATGGCAAG
  TCTGTCATGTATCAGAAAGAACTCAAAGCCCTGGCAGGGTGGGCGCTCCATGTTGGAGACCATCAAGAAG
  GGTGGAGTTAGTGGGTTGACTGGAGAGCTAGAATTTGGAGAAAATGGAGGCAATCCCAATGTCCACTTTG
  AAATCCTTGGAACCAACTATGGAGAAGAGCTTGGCAGAGGTGTTCGAAAACTTGGTTGCTGGAATCCTGT
  CACAGGTCTGAATGGGTCACTGACTGACAAGAAATTGGAGAATAACATGCGTGGAGTGGTTCTACGTGTA
  GTAACTGTTCTGGAAGAACCTTTTGTGATGGTCTCTGAAAATGTCTTGGGTAAGCCGAAGAAATACCAGG
  GCTTCTCCATTGATGTTTTGGATGCCTTATCTAACTACCTGGGTTTTAACTACGAAATTTACGTAGCACC
  GGATCACAAATACGGAAGCCCACAAGAAGATGGGACATGGAATGGCTTGGTAGGAGAACTTGTCTTTAAG
  AGAGCCGACATAGGGATTTCTGCTTTAACCATCACTCCAGATCGTGAAAATGTGGTGGACTTTACGACAC
  GTTACATGGACTACTCAGTGGGGGTACTACTTCGAAGGGCTGAAAAGACAGTGGATATGTTTGCCTGTCT
  TGCACCATTTGATCTCTCTCTATGGGCTTGCATTGCTGGCACAGTCCTTCTGGTGGGTCTACTGGTCTAC
  CTCTTGAACTGGCTTAATCCCCCACGATTACAAATGGGATCAATGACGTCTACTACTCTCTACAACTCCA
  TGTGGTTTGTGTATGGATCTTTTGTACAACAAGGCGGGGAAGTCCCGTACACGACTCTGGCTACCCGAAT
  GATGATGGGGGCTTGGTGGCTATTTGCTTTGATTGTTATCTCATCTTACACGGCAAACCTCGCTGCTTTC
  CTCACTATTACACGCATTGAAAGTTCCATCCAGTCTCTCCAGGACCTTTCCAAGCAAACAGAAATCCCTT
  ATGGCACAGTCCTAGACTCTGCGGTATATGAGCATGTCCGCATGAAAGGACTGAATCCTTTTGAGAGGGA
  CAGCATGTATTCCCAAATGTGGCGGATGATCAACCGAAGCAATGGATCGGAGAACAATGTTCTGGAGTCC
  CAGGCAGGCATTCAAAAGGTAAAATATGGAAATTATGCTTTCGTATGGGATGCAGCTGTATTGGAATATG
  TGGCTATCAATGACCCAGATTGTTCCTTTTACACCATTGGAAATACTGTTGCTGATCGGGGATATGGAAT
  TGCATTACAACATGGCAGTCCTTACCGAGATGTTTTTTCACAAAGGATCCTGGAGCTTCAGCAGAATGGT
  GACATGGACATCCTGAAGCACAAATGGTGGCCTAAGAATGGCCAGTGTGACCTGTACTCGTCAGTGGACA
  CAAAGCAGAAAGGAGGCGCCCTGGACATAAAGAGCTTTGCAGGGGTCTTTTGTATCCTGGCTGCTGGAAT
  TGTCCTCTCCTGCTTCATAGCCATGCTGGAGACGTGGTGGAACAAGAGGAAAGGCTCCCGGGTTCCATCA
  AAAGAGGATGACAAGGAAATTGACCTGGAGCACCTCCATAGACGTGTAAATAGCTTGTGCACAGATGACG
  ACAGCCCCCATAAACAGTTTTCCACCTCGTCAATTGATTTGACCCCTCTGGACATTGACACTTTGCCAAC
  ACGACAAGCACTGGAGCAAATCAGTGATTTCAGGAACACTCATATTACCACAACAACCTTTATCCCAGAG
  CAGATCCAGACTCTTAGCCGCACACTGTCAGCTAAAGCTGCTTCTGGTTTCACTTTTGGCAACGTGCCTG
  AGCACCGAACTGGCCCTTTTAGGCACAGGGCACCTAATGGGGGCTTTTTCAGGAGTCCTATAAAAACAAT
  GTCATCTATTCCTTATCAACCAACTCCTACCCTGGGGCTCAATCTGGGTAATGATCCAGACCGAGGCACC
  TCCATATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286838
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001286838.1, NP_001273767.1
• RefSeq Size: 5069 bp
• RefSeq ORF: 2739 bp
• Locus ID: 2895
• Cytogenetics: 4q22.1-q22.2
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction
• Gene Summary: 'The protein encoded by this gene is a member of the family of ionotropic glutamate receptors which are the predominant excitatory neurotransmitter receptors in the mammalian brain. The encoded protein is a multi-pass membrane protein that is expressed selectively in cerebellar Purkinje cells. A point mutation in the mouse ortholog, associated with the phenotype named 'lurcher', in the heterozygous state leads to ataxia resulting from selective, cell-autonomous apoptosis of cerebellar Purkinje cells during postnatal development. Mice homozygous for this mutation die shortly after birth from massive loss of mid- and hindbrain neurons during late embryogenesis. This protein also plays a role in synapse organization between parallel fibers and Purkinje cells. Alternate splicing results in multiple transcript variants encoding distinct isoforms. Mutations in this gene cause cerebellar ataxia in humans. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (2) has the same N- and C-termini but is shorter compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.