TBC1D4 (NM_001286659) Human Untagged Clone

CAT#: SC337962

TBC1D4 (untagged) - Human TBC1 domain family, member 4 (TBC1D4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBC1D4
• Synonyms: AS160; NIDDM5
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001286659, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCAG
  CTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGA
  CCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCGCAGCCAGAAGCCCGAG
  GCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCG
  TCCCCGCGCCGGGCGCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCGGTATT
  CATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCCACGACCTCACCTACTTTGCC
  TACCTGATCAAGGCGCAGCCCGACGACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACC
  CCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCATGAAAGAGGATGCCAA
  ACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTG
  ACCGTGACCCACAAGAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGCACGAAC
  AGCAGCGCCTGAAGATCCAAGGGGAGCAGCGCGGTCCGGACCCAGGAGAGGACCTGGCTGACTTGGAGGT
  GGTGGTGCCCGGGTCCCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTA
  CCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGGATTTTGGAAGATT
  CTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCA
  CGAGGGCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCCTCGGAC
  TCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGAGATTAACCTTATCAGTCCAGACACTA
  AATCAGTTGTGCTAGAAAAGAATTTTAAAGATATCTCCTCTTGTTCTCAGGGTATAAAGCATGTGGATCA
  CTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTGTTATGTATTCCAGTGT
  GCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGC
  AGAGTGCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTGTGAAAG
  GATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGAGGCATCTCTCATCACTGACAGATAAT
  GAGCAAGCTGACATCTTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTG
  TGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACATCGGGGAAGGCCCTTC
  TACTATTTCAAATAGTACAATCCCAGAAAATGCAACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAA
  AATAAAGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAATGAGAG
  GTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTTGCTTCAGAGAAGGACTACTCACCAGG
  GGATTCTCCACCAGGGACACCGCCAGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGAT
  TCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCAAGCACAAAGAGAAAGC
  TGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCGTTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTG
  CAGTGATGGAGAAGGGAGAAAAAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGTGTGGGAGGAACC
  TCTGTCACTCCTCGCCGGATCTCCTGGCGGCAGCGCATTTTCCTCAGGGTTGCTTCTCCCATGAACAAAT
  CTCCCTCAGCAATGCAACAGCAAGATGGATTGGACAGGAACGAGCTGCTGCCACTGTCCCCCCTCTCTCC
  AACCATGGAGGAGGAACCGCTGGTTGTATTCCTGTCTGGGGAGGATGACCCAGAAAAGATTGAAGAAAGA
  AAGAAATCAAAAGAACTGAGGAGCTTGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATGG
  AAAAAGAAAACCAGAAACTTGAAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGTTAAATTAGACTATGA
  AGAAGTTGGTGCATGTCAGAAAGAGGTCTTAATAACTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAA
  ATCAGATGTGATATGGAAGATATTCATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGGAGAAA
  TTTGGCAGTTTCTGGCTTTACAGTACCGACTCAGACACAGATTGCCTAATAAACAACAGCCTCCTGACAT
  ATCCTATAAGGAACTTTTGAAGCAGCTCACTGCTCAGCAGCATGCGATTCTCGTGGATTTAGGAAGGACG
  TTTCCTACTCACCCTTACTTTTCAGTACAGCTTGGGCCAGGACAGCTGTCACTGTTTAACCTCCTGAAAG
  CCTATTCTTTGCTGGACAAAGAAGTGGGATACTGTCAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCT
  GCACATGAGTGAAGAGCAAGCCTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCAAGCAG
  TACAGACCTGACATGATGTCGCTGCAGATTCAAATGTACCAGCTGTCCAGGCTCCTTCATGACTATCACA
  GAGATCTCTACAATCACCTTGAAGAAAATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCAC
  ATTGTTTGCCTCTCAGTTTTCATTAGGATTTGTAGCCAGAGTTTTTGATATTATTTTTCTTCAGGGAACT
  GAAGTTATATTCAAGGTTGCACTCAGCCTACTGAGCAGCCAAGAGACACTTATAATGGAATGTGAGAGCT
  TTGAAAATATTGTTGAGTTTCTTAAAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTAT
  TACCCAGGTTTTTGAGATGGATATTTCTAAGCAGTTGCATGCCTATGAGGTGGAATATCATGTGCTACAG
  GATGAGCTTCAGGAATCTTCATATTCCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATA
  GCCAACTGAAAAGACAAAACATGGACCTCCTAGAAAAATTACAGGTAGCTCATACTAAAATCCAGGCCTT
  GGAATCAAACCTGGAAAATCTTTTGACGAGAGAGACCAAAATGAAGTCTTTAATCCGGACCCTGGAACAA
  GAAAAAATGGCTTATCAAAAGACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGTCAATT
  GTGACCTGTTGCTGAGAGACCTAAACTGCAACCCTAACAACAAAGCCAAGATAGGAAATAAGCCATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001286659
• ORF Size: 3708 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info

Reference Data

• RefSeq: NM_001286659.1, NP_001273588.1
• RefSeq Size: 6200
• RefSeq ORF: 3708
• Locus ID: 9882
• Gene Summary: This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (3, also known as AS160_v2, PMID:18771725) lacks two alternate in-frame exons in the central coding region, compared to variant 1. The encoded isoform (3) is shorter than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.