KDM6A (NM_001291417) Human Untagged Clone

CAT#: SC338016

KDM6A (untagged) - Human lysine (K)-specific demethylase 6A (KDM6A), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KDM6A
• Synonyms: bA386N14.2; KABUK2; UTX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001291417, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCTGCCGCCGCCGCTTTCGGTGATGAGGAAAAGA
  AAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGCCTGACAGCCGAGGAGAGGGA
  GGCGCTCGGCGGACTGGACAGCCGCCTCTTTGGGTTCGTGAGATTTCATGAAGATGGCGCCAGGACGAAG
  GCCCTACTGGGCAAGGCTGTTCGCTGCTATGAATCTCTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTG
  ATTTCTTTTGTCAATTAGGTCACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGCATACCA
  GAGGTACTACAGTTTACAGTCTGACTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTAC
  TTCCATTATAATGCATTTCAGTGGGCAATTAAAGCATTTCAGGAGGTGCTTTATGTTGATCCCAGCTTTT
  GTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAACACAGACTATGAGTCTAGTTT
  AAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGCACTTTGTCCAATGCTGAAATTCAATTTCAC
  ATTGCCCACTTATATGAAACCCAGAGGAAATATCATTCTGCAAAAGAAGCTTATGAACAACTTTTGCAGA
  CAGAGAATCTTTCTGCACAAGTAAAAGCAACTGTCTTACAACAGTTAGGTTGGATGCATCACACTGTAGA
  TCTCCTGGGAGATAAAGCCACCAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCCTTGGAAGCAGAT
  CCTAATTCTGGCCAGTCCTGGTATTTCCTCGGAAGGTGCTATTCAAGTATTGGGAAAGTTCAGGATGCCT
  TTATATCTTACAGGCAGTCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGGTGTGCT
  ATATCAGCAGCAAAATCAGCCCATGGATGCTTTACAGGCCTATATTTGTGCTGTACAATTGGACCATGGC
  CATGCTGCAGCCTGGATGGACCTAGGCACTCTCTATGAATCCTGCAACCAGCCTCAGGATGCCATTAAAT
  GCTACTTAAATGCAACTAGAAGCAAAAGTTGTAGTAATACCTCTGCACTTGCAGCACGAATTAAGTATTT
  ACAGAATACTTCTGACAATTGGAGTGGTGGACATGCTGTGTCACATCCTCCAGTACAGCAACAAGCTCAT
  TCATGGTGTTTGACACCACAGAAATTACAGCATTTGGAACAGCTCCGCGCAAATAGAAATAATTTAAATC
  CAGCACAGAAACTGATGCTGGAACAGCTGGAAAGTCAGTTTGTCTTAATGCAACAACACCAAATGAGACC
  AACAGGAGTTGCACAGGTACGATCTACTGGAATTCCTAATGGGCCAACAGCTGACTCATCACTGCCTACA
  AACTCAGTCTCTGGCCAGCAGCCACAGCTTGCTCTGACCAGAGTGCCTAGCGTCTCTCAGCCTGGAGTCC
  GTCCTGCCTGCCCTGGGCAGCCTTTGGCCAATGGACCCTTTTCTGCAGGCCATGTTCCCTGTAGCACATC
  AAGAACGCTGGGAAGTACAGACACTATTTTGATAGGCAATAATCATATAACAGGAAGTGGAAGTAATGGA
  AACGTGCCTTACCTGCAGCGAAACGCACTCACTCTACCTCATAACCGCACAAACCTGACCAGCAGCGCAG
  AGGAGCCGTGGAAAAACCAACTATCTAACTCCACTCAGGGGCTTCACAAAGGTCAGAGTTCACATTCGGC
  AGGTCCTAATGGTGAACGACCTCTCTCTTCCACTGGGCCTTCCCAGCATCTCCAGGCAGCTGGCTCTGGT
  ATTCAGAATCAGAACGGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGGGCTGCTCTCAATCACC
  TCTCCTCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAGAGAGCAAGCCTTCAGG
  AAACATATTGACGGTGCCTGAAACAAGCAGGCACACTGGAGAGACACCTAACAGCACTGCCAGTGTCGAG
  GGACTTCCTAATCATGTCCATCAGATGACGGCAGATGCTGTTTGCAGTCCTAGCCATGGAGATTCTAAGT
  CACCAGGTTTACTAAGTTCAGACAATCCTCAGCTCTCTGCCTTGTTGATGGGAAAAGCCAATAACAATGT
  GGGTACTGGAACCTGTGACAAAGTCAATAACATCCACCCAGCTGTTCATACAAAGACTGATAACTCTGTT
  GCCTCTTCACCATCTTCAGCCATTTCAACAGCAACACCTTCTCCAAAATCCACTGAGCAGACAACCACAA
  ACAGTGTTACCAGCCTTAACAGCCCTCACAGTGGGCTACACACAATTAATGGAGAAGGGATGGAAGAATC
  TCAGAGCCCCATGAAAACAGATCTGCTTCTGGTTAACCACAAACCTAGTCCACAGATCATACCATCAATG
  TCTGTGTCCATATACCCCAGCTCAGCAGAAGTTCTGAAGGCATGCAGGAATCTAGGTAAAAATGGCTTAT
  CTAACAGTAGCATTTTGTTGGATAAATGTCCACCTCCAAGACCACCATCTTCACCATACCCTCCCTTGCC
  AAAGGACAAGTTGAATCCACCTACACCTAGTATTTACTTGGAAAATAAACGTGATGCTTTCTTTCCTCCA
  TTACATCAATTTTGTACAAATCCGAACAACCCTGTTACAGTAATACGTGGCCTTGCTGGAGCTCTTAAGT
  TAGACCTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATATGGTAGAAGTGAGGAC
  ACAGTTGTTGCAGCCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAATCTGGCATTGTGAAAGT
  AATAGATCTCATACTACAATTGCTAAATATGCACAGTACCAGGCCTCCTCATTCCAGGAATCATTGAGAG
  AAGAAAATGAAAAAAGAAGTCATCATAAAGACCACTCAGATAGTGAATCTACATCGTCAGATAATTCTGG
  GAGGAGGAGGAAAGGACCCTTTAAAACCATAAAGTTTGGGACCAATATTGACCTATCTGATGACAAAAAG
  TGGAAGTTGCAGCTACATGAGCTGACTAAACTTCCTGCTTTTGTGCGTGTCGTATCAGCAGGAAATCTTC
  TAAGCCATGTTGGTCATACCATATTGGGCATGAACACAGTTCAACTATACATGAAAGTTCCAGGGAGCAG
  AACACCAGGTCATCAGGAAAATAACAACTTCTGTTCAGTTAACATAAATATTGGCCCAGGTGACTGTGAA
  TGGTTTGTTGTTCCTGAAGGTTACTGGGGTGTTCTGAATGACTTCTGTGAAAAAAATAATTTGAATTTCC
  TAATGGGTTCTTGGTGGCCCAATCTTGAAGATCTTTATGAAGCAAATGTTCCAGTGTATAGGTTTATTCA
  GCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTTCATTGGGTTCAGGCTATTGGCTGGTGCAAC
  AACATTGCTTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTGGAACGGTACGAATGGA
  ACAAATTGCAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATATGGCACGAAATATCAA
  GGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTAAGAACTCTGAAGCAATGTCAGACA
  TTGAGGGAAGCTCTCATTGCTGCAGGAAAAGAGATTATATGGCATGGGCGGACAAAAGAAGAACCAGCTC
  ATTACTGTAGCATTTGTGAAGTGGAGGTTTTTGATCTGCTTTTTGTCACTAATGAGAGTAATTCACGAAA
  GACCTACATAGTACATTGCCAAGATTGTGCACGAAAAACAAGCGGAAACTTGGAAAACTTTGTGGTGCTA
  GAACAGTACAAAATGGAGGACCTGATGCAAGTCTATGACCAATTTACATTAGCTCCTCCATTACCATCCG
  CCTCATCTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291417
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291417.1, NP_001278346.1
• RefSeq Size: 5650 bp
• RefSeq ORF: 4071 bp
• Locus ID: 7403
• Cytogenetics: Xp11.3
• Gene Summary: 'This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (4) lacks two consecutive in-frame exons in the coding region, compared to variant 1. The resulting isoform (4) lacks an internal segment, compared to isoform 1.