ROBO2 (NM_001290039) Human Untagged Clone

CAT#: SC338022

ROBO2 (untagged) - Human roundabout, axon guidance receptor, homolog 2 (Drosophila) (ROBO2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ROBO2
• Synonyms: SAX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001290039, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAGTCTGCTGATGTTTACACAACTACTGCTCTGTGGATTTTTATATGTTCGGGTTGATGGATCGCGTC
  TTCGCCAGGAGGACTTTCCCCCGCGGATTGTGGAGCATCCTTCCGATGTCATCGTCTCTAAGGGCGAGCC
  CACGACTCTGAACTGCAAGGCGGAGGGCCGGCCAACGCCCACCATTGAGTGGTACAAAGATGGGGAGCGA
  GTGGAGACTGACAAGGACGATCCCCGGTCCCACAGGATGCTTCTGCCCAGCGGATCCTTATTCTTCTTGC
  GCATCGTGCACGGGCGCAGGAGTAAACCTGATGAAGGAAGCTACGTTTGTGTTGCGAGGAACTATCTTGG
  TGAAGCAGTGAGTCGAAATGCGTCTCTGGAAGTGGCATTGTTACGAGATGACTTCCGACAAAACCCCACA
  GATGTTGTAGTGGCAGCTGGAGAGCCTGCAATCCTGGAGTGCCAGCCTCCCCGGGGACACCCAGAACCCA
  CCATCTACTGGAAAAAAGACAAAGTTCGAATTGATGACAAGGAAGAAAGAATAAGTATCCGTGGTGGAAA
  ACTGATGATCTCCAATACCAGGAAAAGTGATGCAGGGATGTATACTTGTGTTGGTACCAATATGGTGGGA
  GAAAGGGACAGTGACCCAGCAGAGCTGACTGTCTTTGAACGACCCACATTTCTCAGGAGGCCAATTAACC
  AGGTGGTACTGGAGGAAGAAGCTGTAGAATTTCGTTGTCAAGTCCAAGGAGATCCTCAACCAACTGTGAG
  GTGGAAAAAGGATGATGCAGACTTGCCAAGAGGAAGGTATGACATCAAAGACGATTACACACTAAGAATT
  AAAAAGACCATGAGTACAGATGAAGGCACCTATATGTGTATTGCTGAGAATCGGGTTGGAAAAATGGAAG
  CCTCTGCTACACTCACCGTCCGAGCTCGCCCTGTTGCTCCCCCACAGTTTGTGGTTCGGCCAAGAGATCA
  GATTGTTGCTCAAGGTCGAACAGTGACATTTCCCTGTGAAACTAAAGGAAACCCACAGCCAGCTGTTTTT
  TGGCAGAAAGAAGGCAGCCAGAACCTACTTTTCCCAAACCAACCCCAGCAGCCCAACAGTAGATGCTCAG
  TGTCACCAACTGGAGACCTCACAATCACCAACATTCAACGTTCCGACGCGGGTTACTACATCTGCCAGGC
  TTTAACTGTGGCAGGAAGCATTTTAGCAAAAGCTCAACTGGAGGTTACTGATGTTTTGACAGATAGACCT
  CCACCTATAATTCTACAAGGCCCAGCCAACCAAACGCTGGCAGTGGATGGTACAGCGTTACTGAAATGTA
  AAGCCACTGGTGATCCTCTTCCTGTAATTAGCTGGTTAAAGGAGGGATTTACTTTTCCGGGTAGAGATCC
  AAGAGCAACAATTCAAGAGCAAGGCACACTGCAGATTAAGAATTTACGGATTTCTGATACTGGCACTTAT
  ACTTGTGTGGCTACAAGTTCAAGTGGAGAGACTTCCTGGAGTGCAGTGCTGGATGTGACAGAGTCTGGAG
  CAACAATCAGTAAAAACTATGATTTAAGTGACCTGCCAGGGCCACCATCCAAACCGCAGGTCACTGATGT
  TACTAAGAACAGTGTCACCTTGTCCTGGCAGCCAGGTACCCCTGGAACCCTTCCAGCAAGTGCATATATC
  ATTGAGGCTTTCAGCCAATCAGTGAGCAACAGCTGGCAGACCGTGGCAAACCATGTAAAGACCACCCTCT
  ATACTGTAAGAGGACTGCGGCCCAATACAATCTACTTATTCATGGTCAGAGCGATCAACCCCCAAGGTCT
  CAGTGACCCAAGTCCCATGTCAGATCCTGTGCGCACACAAGATATCAGCCCACCAGCACAAGGAGTGGAC
  CACAGGCAAGTGCAGAAAGAGCTAGGAGATGTCCTTGTCCGTCTTCATAATCCAGTTGTGCTGACTCCCA
  CCACGGTTCAGGTCACATGGACGGTTGATCGCCAACCCCAGTTTATCCAAGGCTACCGAGTGATGTATCG
  TCAGACTTCAGGTCTGCAGGCGACATCTTCGTGGCAGAATTTAGATGCCAAAGTCCCGACTGAACGAAGT
  GCTGTCTTAGTCAACCTGAAAAAGGGGGTGACTTATGAAATTAAAGTACGGCCATATTTTAATGAGTTCC
  AAGGAATGGATAGTGAATCTAAAACGGTTCGTACTACTGAAGAAGCCCCAAGTGCCCCACCACAGTCTGT
  CACTGTACTGACAGTTGGAAGCTACAATAGCACAAGTATTAGTGTTTCCTGGGATCCTCCTCCTCCAGAT
  CACCAGAATGGAATTATCCAAGAATACAAGATCTGGTGTCTAGGAAATGAAACGCGATTCCATATCAACA
  AAACTGTGGATGCAGCCATTCGGTCCGTAATAATTGGTGGATTATTCCCAGGTATTCAATACCGGGTAGA
  GGTTGCAGCTAGTACCAGTGCAGGGGTTGGAGTAAAGAGTGAGCCACAGCCAATAATAATCGGGAGACGC
  AATGAAGTTGTCATTACTGAAAACAATAACAGCATAACTGAGCAAATCACTGATGTGGTGAAGCAACCAG
  CCTTTATAGCTGGTATTGGTGGTGCCTGCTGGGTAATTCTGATGGGTTTTAGCATATGGTTGTATTGGCG
  AAGAAAGAAGAGGAAGGGACTCAGTAATTATGCTGTTACGTTTCAAAGAGGAGATGGAGGACTAATGAGC
  AATGGAAGCCGTCCAGGTCTTCTCAATGCTGGTGATCCCAGCTATCCATGGCTTGCTGATTCTTGGCCAG
  CCACGAGCTTGCCAGTAAATAATAGCAACAGTGGCCCAAATGAGATTGGAAATTTTGGCCGTGGAGATGT
  GCTGCCACCAGTTCCAGGCCAAGGGGATAAAACAGCAACGATGCTCTCAGATGGAGCCATTTATAGTAGC
  ATTGACTTCACTACCAAAACCAGTTACAACAGTTCCAGCCAAATAACACAGGCTACCCCATATGCCACGA
  CACAGATCTTGCATTCCAACAGCATACATGAATTGGCTGTCGATCTGCCTGATCCACAATGGAAAAGCTC
  AATTCAGCAAAAAACAGATCTGATGGGATTTGGTTATTCTCTACCTGATCAGAACAAAGGTAACAATGGT
  GGGAAAGGTGGAAAAAAGAAGAAAAATAAAAACTCTTCTAAACCACAGAAAAACAATGGATCCACTTGGG
  CCAATGTCCCTCTACCTCCCCCCCCAGTCCAGCCCCTTCCTGGCACGGAGCTGGAACACTATGCAGTGGA
  ACAACAAGAAAATGGCTATGACAGTGATAGCTGGTGCCCACCATTGCCAGTACAAACTTACTTACACCAA
  GGTCTGGAAGATGAACTGGAAGAAGATGATGATAGGGTCCCAACACCTCCTGTTCGAGGCGTGGCTTCTT
  CTCCTGCTATCTCCTTTGGACAGCAGTCCACTGCAACTCTTACTCCATCCCCACGGGAAGAGATGCAACC
  CATGCTGCAGGCTCACCTGGATGAGTTGACAAGAGCCTATCAGTTTGATATAGCAAAACAAACATGGCAC
  ATTCAAAGCAATAATCAACCTCCACAGCCTCCAGTTCCACCGTTAGGTTATGTGTCTGGAGCCTTGATTT
  CTGATTTGGAAACGGATGTTGCAGATGATGATGCCGACGACGAAGAGGAAGCTTTAGAAATCCCCAGGCC
  CCTGAGAGCACTGGACCAGACTCCTGGATCCAGCATGGACAATCTAGACAGCTCTGTGACAGGAAAAGCC
  TTTACCTCCTCTCAAAGACCTCGACCTACCAGCCCATTTTCTACTGACAGTAACACCAGTGCAGCCCTGA
  GTCAAAGTCAGAGGCCTCGGCCCACTAAAAAACACAAGGGAGGGCGGATGGACCAACAACCAGCATTGCC
  TCATCGAAGGGAAGGAATGACAGATGAGGAGGCCTTGGTGCCCTATAGCAAGCCCAGTTTCCCATCTCCA
  GGTGGCCACAGCTCATCAGGAACAGCTTCTTCTAAGGGATCCACTGGACCTAGGAAAACCGAGGTGTTGA
  GAGCAGGCCACCAGCGCAATGCCAGCGACCTTCTTGACATAGGATATATGGGCTCCAACAGTCAAGGACA
  GTTTACAGGTGAATTATAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001290039
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001290039.1, NP_001276968.1
• RefSeq Size: 8701 bp
• RefSeq ORF: 4149 bp
• Locus ID: 6092
• Cytogenetics: 3p12.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: 'The protein encoded by this gene belongs to the ROBO family, part of the immunoglobulin superfamily of proteins that are highly conserved from fly to human. The encoded protein is a transmembrane receptor for the slit homolog 2 protein and functions in axon guidance and cell migration. Mutations in this gene are associated with vesicoureteral reflux, characterized by the backward flow of urine from the bladder into the ureters or the kidney. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2014]'
  Transcript Variant: This variant (3) lacks an alternate in-frame exon in the 3' coding region compared to variant 4. The encoded protein (isoform 3) is shorter than variant 4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.