KDM6A (NM_001291416) Human Untagged Clone

CAT#: SC338026

KDM6A (untagged) - Human lysine (K)-specific demethylase 6A (KDM6A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KDM6A
• Synonyms: bA386N14.2; KABUK2; UTX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001291416, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAATCCTGCGGAGTGTCGCTCGCTACCGCCGCCGCTGCCGCCGCCGCTTTCGGTGATGAGGAAAAGA
  AAATGGCGGCGGGAAAAGCGAGCGGCGAGAGCGAGGAGGCGTCCCCCAGCCTGACAGCCGAGGAGAGGGA
  GGCGCTCGGCGGACTGGACAGCCGCCTCTTTGGGTTCGTGAGATTTCATGAAGATGGCGCCAGGACGAAG
  GCCCTACTGGGCAAGGCTGTTCGCTGCTATGAATCTCTAATCTTAAAAGCTGAAGGAAAAGTGGAGTCTG
  ATTTCTTTTGTCAATTAGGTCACTTCAACCTCTTATTGGAAGATTATCCAAAAGCATTATCTGCATACCA
  GAGGTACTACAGTTTACAGTCTGACTACTGGAAGAATGCTGCCTTTTTATATGGTCTTGGTTTGGTCTAC
  TTCCATTATAATGCATTTCAGTGGGCAATTAAAGCATTTCAGGAGGTGCTTTATGTTGATCCCAGCTTTT
  GTCGAGCCAAGGAAATTCATTTACGACTTGGGCTTATGTTCAAAGTGAACACAGACTATGAGTCTAGTTT
  AAAGCATTTTCAGTTAGCTTTGGTTGACTGTAATCCCTGCACTTTGTCCAATGCTGAAATTCAATTTCAC
  ATTGCCCACTTATATGAAACCCAGAGGAAATATCATTCTGCAAAAGAAGCTTATGAACAACTTTTGCAGA
  CAGAGAATCTTTCTGCACAAGTAAAAGCAACTGTCTTACAACAGTTAGGTTGGATGCATCACACTGTAGA
  TCTCCTGGGAGATAAAGCCACCAAGGAAAGCTATGCTATTCAGTATCTCCAAAAGTCCTTGGAAGCAGAT
  CCTAATTCTGGCCAGTCCTGGTATTTCCTCGGAAGGTGCTATTCAAGTATTGGGAAAGTTCAGGATGCCT
  TTATATCTTACAGGCAGTCTATTGATAAATCAGAAGCAAGTGCAGATACATGGTGTTCAATAGGTGTGCT
  ATATCAGCAGCAAAATCAGCCCATGGATGCTTTACAGGCCTATATTTGTGCTGTACAATTGGACCATGGC
  CATGCTGCAGCCTGGATGGACCTAGGCACTCTCTATGAATCCTGCAACCAGCCTCAGGATGCCATTAAAT
  GCTACTTAAATGCAACTAGAAGCAAAAGTTGTAGTAATACCTCTGCACTTGCAGCACGAATTAAGTATTT
  ACAGGCATGTAAACCTCATCATCCAAATACTGAACCTGTATTAGGCCTCAGTCAAACACCAATTTCACAG
  CAATCCTTGCCACTACACATGATTCCTTCTAGCCAAGTAGATGACCTGTCCAGTCCTGCCAAGAGGAAAA
  GAACATCTAGTCCAACAAAGAATACTTCTGACAATTGGAGTGGTGGACATGCTGTGTCACATCCTCCAGT
  ACAGCAACAAGCTCATTCATGGTGTTTGACACCACAGAAATTACAGCATTTGGAACAGCTCCGCGCAAAT
  AGAAATAATTTAAATCCAGCACAGAAACTGATGCTGGAACAGCTGGAAAGTCAGTTTGTCTTAATGCAAC
  AACACCAAATGAGACCAACAGGAGTTGCACAGGTACGATCTACTGGAATTCCTAATGGGCCAACAGCTGA
  CTCATCACTGCCTACAAACTCAGTCTCTGGCCAGCAGCCACAGCTTGCTCTGACCAGAGTGCCTAGCGTC
  TCTCAGCCTGGAGTCCGTCCTGCCTGCCCTGGGCAGCCTTTGGCCAATGGACCCTTTTCTGCAGGCCATG
  TTCCCTGTAGCACATCAAGAACGCTGGGAAGTACAGACACTATTTTGATAGGCAATAATCATATAACAGG
  AAGTGGAAGTAATGGAAACGTGCCTTACCTGCAGCGAAACGCACTCACTCTACCTCATAACCGCACAAAC
  CTGACCAGCAGCGCAGAGGAGCCGTGGAAAAACCAACTATCTAACTCCACTCAGGGGCTTCACAAAGGTC
  AGAGTTCACATTCGGCAGGTCCTAATGGTGAACGACCTCTCTCTTCCACTGGGCCTTCCCAGCATCTCCA
  GGCAGCTGGCTCTGGTATTCAGAATCAGAACGGACATCCCACCCTGCCTAGCAATTCAGTAACACAGGGG
  GCTGCTCTCAATCACCTCTCCTCTCACACTGCTACCTCAGGTGGACAACAAGGCATTACCTTAACCAAAG
  AGAGCAAGCCTTCAGGAAACATATTGACGGTGCCTGAAACAAGCAGGCACACTGGAGAGACACCTAACAG
  CACTGCCAGTGTCGAGGGACTTCCTAATCATGTCCATCAGATGACGGCAGATGCTGTTTGCAGTCCTAGC
  CATGGAGATTCTAAGTCACCAGGTTTACTAAGTTCAGACAATCCTCAGCTCTCTGCCTTGTTGATGGGAA
  AAGCCAATAACAATGTGGGTACTGGAACCTGTGACAAAGTCAATAACATCCACCCAGCTGTTCATACAAA
  GACTGATAACTCTGTTGCCTCTTCACCATCTTCAGCCATTTCAACAGCAACACCTTCTCCAAAATCCACT
  GAGCAGACAACCACAAACAGTGTTACCAGCCTTAACAGCCCTCACAGTGGGCTACACACAATTAATGGAG
  AAGGGATGGAAGAATCTCAGAGCCCCATGAAAACAGATCTGCTTCTGGTTAACCACAAACCTAGTCCACA
  GATCATACCATCAATGTCTGTGTCCATATACCCCAGCTCAGCAGAAGTTCTGAAGGCATGCAGGAATCTA
  GGTAAAAATGGCTTATCTAACAGTAGCATTTTGTTGGATAAATGTCCACCTCCAAGACCACCATCTTCAC
  CATACCCTCCCTTGCCAAAGGACAAGTTGAATCCACCTACACCTAGTATTTACTTGGAAAATAAACGTGA
  TGCTTTCTTTCCTCCATTACATCAATTTTGTACAAATCCGAACAACCCTGTTACAGTAATACGTGGCCTT
  GCTGGAGCTCTTAAGTTAGACCTGGGACTTTTCTCTACTAAAACTTTGGTGGAAGCTAACAATGAACATA
  TGGTAGAAGTGAGGACACAGTTGTTGCAGCCAGCAGATGAAAACTGGGATCCCACTGGAACAAAGAAAAT
  CTGGCATTGTGAAAGTAATAGATCTCATACTACAATTGCTAAATATGCACAGTACCAGGCCTCCTCATTC
  CAGGAATCATTGAGAGAAGAAAATGAAAAAAGAAGTCATCATAAAGACCACTCAGATAGTGAATCTACAT
  CGTCAGATAATTCTGGGAGGAGGAGGAAAGGACCCTTTAAAACCATAAAGTTTGGGACCAATATTGACCT
  ATCTGATGACAAAAAGTGGAAGTTGCAGCTACATGAGCTGACTAAACTTCCTGCTTTTGTGCGTGTCGTA
  TCAGCAGGAAATCTTCTAAGCCATGTTGGTCATACCATATTGGGCATGAACACAGTTCAACTATACATGA
  AAGTTCCAGGGAGCAGAACACCAGGTCATCAGGAAAATAACAACTTCTGTTCAGTTAACATAAATATTGG
  CCCAGGTGACTGTGAATGGTTTGTTGTTCCTGAAGGTTACTGGGGTGTTCTGAATGACTTCTGTGAAAAA
  AATAATTTGAATTTCCTAATGGGTTCTTGGTGGCCCAATCTTGAAGATCTTTATGAAGCAAATGTTCCAG
  TGTATAGGTTTATTCAGCGACCTGGAGATTTGGTCTGGATAAATGCAGGCACTGTTCATTGGGTTCAGGC
  TATTGGCTGGTGCAACAACATTGCTTGGAATGTTGGTCCACTTACAGCCTGCCAGTATAAATTGGCAGTG
  GAACGGTACGAATGGAACAAATTGCAAAGTGTGAAGTCAATAGTACCCATGGTTCATCTTTCCTGGAATA
  TGGCACGAAATATCAAGGTCTCAGATCCAAAGCTTTTTGAAATGATTAAGTATTGTCTTCTAAGAACTCT
  GAAGCAATGTCAGACATTGAGGGAAGCTCTCATTGCTGCAGGAAAAGAGATTATATGGCATGGGCGGACA
  AAAGAAGAACCAGCTCATTACTGTAGCATTTGTGAAGTGGAGGTTTTTGATCTGCTTTTTGTCACTAATG
  AGAGTAATTCACGAAAGACCTACATAGTACATTGCCAAGATTGTGCACGAAAAACAAGCGGAAACTTGGA
  AAACTTTGTGGTGCTAGAACAGTACAAAATGGAGGACCTGATGCAAGTCTATGACCAATTTACATTAGCT
  CCTCCATTACCATCCGCCTCATCTTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001291416
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001291416.1, NP_001278345.1
• RefSeq Size: 5806 bp
• RefSeq ORF: 4227 bp
• Locus ID: 7403
• Cytogenetics: Xp11.3
• Gene Summary: 'This gene is located on the X chromosome and is the corresponding locus to a Y-linked gene which encodes a tetratricopeptide repeat (TPR) protein. The encoded protein of this gene contains a JmjC-domain and catalyzes the demethylation of tri/dimethylated histone H3. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (2) lacks an in-frame exon in the coding region, compared to variant 1. The resulting isoform (2) lacks an internal segment, compared to isoform 1.