ATP7A (NM_001282224) Human Untagged Clone

CAT#: SC338030

ATP7A (untagged) - Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP7A
• Synonyms: DSMAX; MK; MNK; SMAX3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001282224, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCCAAGTATGGGTGTGAATTCTGTTACCATTTCTGTTGAGGGTATGACTTGCAATTCCTGTGTTT
  GGACCATTGAGCAGCAGATTGGAAAAGTGAATGGTGTGCATCACATTAAGGTATCACTGGAAGAAAAAAA
  TGCAACTATTATTTATGACCCTAAACTACAGACTCCAAAGACCCTACAGGAAGCTATTGATGACATGGGC
  TTTGATGCTGTTATCCATAATCCTGACCCTCTCCCTGTTTTAACTGACACCTTGTTTCTGACTGTTACGG
  CGTCACTGACTTTGCCATGGGACCATATCCAAAGCACATTGCTGAAGACCAAGGGTGTGACAGACATTAA
  AATTTACCCTCAGAAAAGAACTGTAGCAGTGACAATAATCCCTTCTATAGTGAATGCCAATCAGATAAAA
  GAGCTGGTTCCAGAACTCAGTTTAGATACTGGGACACTGGAGAAAAAGTCAGGAGCTTGTGAAGATCATA
  GTATGGCTCAAGCTGGTGAAGTCGTGCTGAAGATGAAAGTGGAAGGGATGACCTGCCATTCATGTACTAG
  CACTATTGAAGGAAAAATTGGGAAACTGCAAGGTGTTCAGCGAATTAAAGTCTCCCTGGACAATCAAGAA
  GCTACTATTGTTTATCAACCTCATCTTATCTCAGTAGAGGAAATGAAAAAGCAGATTGAAGCTATGGGCT
  TTCCAGCATTTGTCAAAAAGCAGCCCAAGTACCTCAAATTGGGAGCTATTGATGTAGAACGTCTAAAGAA
  CACACCAGTTAAATCCTCAGAAGGGTCACAGCAAAGGAGTCCATCATATACCAATGATTCAACAGCCACT
  TTCATCATTGATGGCATGCATTGTAAATCATGTGTGTCAAATATTGAAAGTACTTTATCTGCACTCCAAT
  ATGTAAGCAGCATAGTAGTTTCTTTAGAGAATAGGTCTGCCATTGTGAAGTATAATGCAAGCTCAGTCAC
  TCCAGAATCCCTGAGAAAAGCAATAGAGGCTGTATCACCGGGGCTATATAGAGTTAGTATCACAAGTGAA
  GTTGAGAGTACCTCAAACTCTCCCTCCAGCTCATCTCTTCAGAAGATTCCTTTGAATGTAGTTAGCCAGC
  CTCTGACACAAGAAACTGTGATAAACATTGATGGCATGACTTGTAATTCCTGTGTGCAGTCTATTGAGGG
  TGTCATATCAAAAAAGCCAGGTGTAAAATCCATACGAGTCTCCCTTGCAAATAGCAATGGGACTGTTGAG
  TATGATCCTCTACTAACCTCTCCAGAAACGTTGAGAGGAGCAATAGAAGACATGGGATTTGATGCTACCT
  TGTCAGACACGAATGAGCCGTTGGTAGTAATAGCTCAGCCTTCATCGGAAATGCCGCTTTTGACTTCAAC
  TAATGAATTTTATACTAAAGGGATGACACCAGTTCAAGACAAGGAGGAAGGAAAGAATTCATCTAAGTGT
  TACATACAGGTCACTGGCATGACTTGCGCTTCCTGTGTAGCAAACATTGAACGGAATTTAAGGCGGGAAG
  AAGGAATATATTCTATACTTGTGGCCCTGATGGCTGGCAAGGCAGAAGTAAGGTATAATCCTGCTGTTAT
  ACAACCCCCAATGATAGCAGAGTTCATCCGAGAACTTGGATTTGGAGCCACTGTGATAGAAAATGCTGAT
  GAAGGAGATGGTGTTTTGGAACTTGTTGTGAGGGGAATGACGTGTGCCTCCTGCGTACATAAAATAGAGT
  CTAGTCTCACAAAACACAGAGGGATCCTATACTGCTCCGTGGCCCTGGCAACCAACAAAGCACATATTAA
  ATATGACCCAGAAATTATTGGTCCTAGAGATATTATCCATACAATTGAAAGCTTAGGTTTTGAAGCTTCT
  TTGGTCAAGAAGGATCGGTCAGCAAGTCACTTAGATCATAAACGAGAAATAAGACAATGGAGACGGTCTT
  TTCTTGTGAGTCTGTTTTTCTGTATTCCTGTAATGGGGCTGATGATATATATGATGGTTATGGACCACCA
  CTTTGCAACTCTTCACCATAATCAAAACATGAGTAAAGAAGAAATGATCAACCTTCATTCTTCTATGTTC
  CTGGAGCGCCAGATTCTTCCAGGATTGTCTGTTATGAATTTGCTGTCCTTTTTATTGTGTGTACCTGTAC
  AGGGCAAAACATCAGAGGCTCTTGCAAAGTTAATTTCACTACAAGCTACAGAAGCAACTATTGTAACTCT
  TGATTCTGATAATATCCTCCTCAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATT
  AAAGTAGTTCCAGGAGGCAAATTTCCAGTGGATGGTCGTGTTATTGAAGGACATTCTATGGTAGATGAGT
  CCCTCATCACAGGGGAGGCAATGCCTGTGGCTAAGAAACCTGGCAGCACAGTGATTGCTGGTTCCATTAA
  CCAGAACGGGTCACTGCTTATCTGCGCAACACATGTTGGAGCAGACACAACCCTTTCTCAAATTGTCAAA
  CTTGTGGAAGAGGCACAAACATCAAAGGCTCCTATCCAGCAGTTTGCAGACAAACTCAGTGGCTATTTTG
  TTCCTTTTATTGTTTTTGTTTCCATTGCCACCCTCTTGGTATGGATTGTAATTGGATTTCTGAATTTTGA
  AATTGTGGAAACCTACTTTCCTGGCTACAATAGAAGTATCTCCCGAACAGAAACGATAATACGATTTGCT
  TTCCAAGCCTCTATCACAGTTCTGTGTATTGCATGTCCCTGTTCACTGGGACTGGCCACTCCAACTGCTG
  TGATGGTGGGTACAGGAGTAGGTGCTCAAAATGGCATACTAATAAAAGGTGGAGAGCCATTGGAGATGGC
  TCATAAGGTAAAGGTAGTGGTATTTGATAAGACTGGAACCATTACTCACGGAACCCCAGTGGTGAATCAA
  GTAAAGGTTCTAACTGAAAGTAACAGAATATCACACCATAAAATCTTGGCCATTGTGGGAACTGCTGAAA
  GTAACAGTGAACACCCTCTAGGAACAGCCATAACCAAATATTGCAAACAGGAGCTGGACACTGAAACCTT
  GGGTACCTGCATAGATTTCCAGGTTGTGCCAGGCTGTGGTATTAGCTGTAAAGTCACCAATATTGAAGGC
  TTGCTACATAAGAATAACTGGAATATAGAGGACAATAATATTAAAAATGCATCCCTGGTTCAAATTGATG
  CCAGTAATGAACAGTCATCAACTTCGTCTTCCATGATTATTGATGCCCAGATCTCAAATGCTCTTAATGC
  TCAGCAGTATAAAGTCCTCATTGGTAACCGGGAGTGGATGATTAGAAATGGTCTTGTCATTAATAACGAT
  GTAAATGATTTCATGACTGAACATGAGAGAAAAGGTCGGACTGCTGTATTAGTAGCAGTTGATGATGAGC
  TGTGTGGCTTGATAGCCATTGCAGACACAGTGAAGCCTGAAGCAGAACTGGCTATCCATATTCTGAAATC
  TATGGGCTTAGAAGTAGTTCTGATGACTGGAGACAACAGTAAAACAGCTAGATCTATTGCTTCTCAGGTT
  GGCATTACTAAGGTGTTTGCTGAAGTTCTACCTTCTCACAAGGTTGCTAAAGTGAAGCAACTTCAAGAGG
  AGGGGAAACGGGTAGCAATGGTGGGAGATGGAATCAATGACTCCCCAGCTCTGGCAATGGCTAATGTGGG
  AATTGCTATTGGCACAGGCACAGATGTAGCCATTGAAGCAGCTGATGTGGTTTTGATAAGGAATGATCTT
  CTGGATGTAGTGGCAAGTATTGACTTATCAAGAAAGACAGTCAAGAGGATTCGGATAAATTTTGTCTTTG
  CTCTAATTTATAATCTGGTTGGAATTCCCATAGCTGCTGGAGTTTTTATGCCCATTGGTTTGGTTTTGCA
  GCCCTGGATGGGATCTGCAGCAATGGCTGCTTCATCTGTTTCTGTAGTACTTTCTTCTCTCTTCCTTAAA
  CTTTACAGGAAACCAACTTACGAGAGTTATGAACTGCCTGCCCGGAGCCAGATAGGACAGAAGAGTCCTT
  CAGAAATCAGCGTTCATGTTGGAATAGATGATACCTCAAGGAATTCTCCTAAACTGGGTTTGCTGGACCG
  GATTGTTAATTATAGCAGAGCCTCTATAAACTCACTACTGTCTGATAAACGCTCCCTAAACAGTGTTGTT
  ACCAGTGAACCTGACAAGCACTCACTCCTGGTGGGAGACTTCAGGGAAGATGATGACACTGCATTATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001282224
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001282224.1, NP_001269153.1
• RefSeq Size: 8306 bp
• RefSeq ORF: 4269 bp
• Locus ID: 538
• Cytogenetics: Xq21.1
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon, compared to variant 1. The encoded isoform (2) is shorter than isoform 1. This variant is supported by data in PMIDs 7490081 and 10970802. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.