DAP Kinase 1 (DAPK1) (NM_001288731) Human Untagged Clone

CAT#: SC338035

DAPK1 (untagged) - Human death-associated protein kinase 1 (DAPK1), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DAPK1
• Synonyms: DAPK; ROCO3
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001288731, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACCGTGTTCAGGCAGGAAAACGTGGATGATTACTACGACACCGGCGAGGAACTTGGCAGTGGACAGT
  TTGCGGTTGTGAAGAAATGCCGTGAGAAAAGCACCGGCCTCCAGTATGCCGCCAAATTCATCAAGAAAAG
  GAGGACTAAGTCCAGCCGGCGGGGTGTGAGCCGCGAGGACATCGAGCGGGAGGTCAGCATCCTGAAGGAG
  ATCCAGCACCCCAATGTCATCACCCTGCACGAGGTCTATGAGAACAAGACGGACGTCATCCTGATCTTGG
  AACTCGTTGCAGGTGGCGAGCTGTTTGACTTCTTAGCTGAAAAGGAATCTTTAACTGAAGAGGAAGCAAC
  TGAATTTCTCAAACAAATTCTTAATGGTGTTTACTACCTGCACTCCCTTCAAATCGCCCACTTTGATCTT
  AAGCCTGAGAACATAATGCTTTTGGATAGAAATGTCCCCAAACCTCGGATCAAGATCATTGACTTTGGGT
  TGGCCCATAAAATTGACTTTGGAAATGAATTTAAAAACATATTTGGGACTCCAGAGTTTGTCGCTCCTGA
  GATAGTCAACTATGAACCTCTTGGTCTTGAGGCAGATATGTGGAGTATCGGGGTAATAACCTATATCCTC
  CTAAGTGGGGCCTCCCCATTTCTTGGAGACACTAAGCAAGAAACGTTAGCAAATGTATCCGCTGTCAACT
  ACGAATTTGAGGATGAATACTTCAGTAATACCAGTGCCCTAGCCAAAGATTTCATAAGAAGACTTCTGGT
  CAAGGATCCAAAGAAGAGAATGACAATTCAAGATAGTTTGCAGCATCCCTGGATCAAGCCTAAAGATACA
  CAACAGGCACTTAGTAGAAAAGCATCAGCAGTAAACATGGAGAAATTCAAGAAGTTTGCAGCCCGGAAAA
  AATGGAAACAATCCGTTCGCTTGATATCACTGTGCCAAAGATTATCCAGGTCATTCCTGTCCAGAAGTAA
  CATGAGTGTTGCCAGAAGCGATGATACTCTGGATGAGGAAGACTCCTTTGTGATGAAAGCCATCATCCAT
  GCCATCAACGATGACAATGTCCCAGGCCTGCAGCACCTTCTGGGCTCATTATCCAACTATGATGTTAACC
  AACCCAACAAGCACGGGACACCTCCATTACTCATTGCTGCTGGCTGTGGGAATATTCAAATACTACAGTT
  GCTCATTAAAAGAGGCTCGAGAATCGATGTCCAGGATAAGGGCGGGTCCAATGCCGTCTACTGGGCTGCT
  CGGCATGGCCACGTCGATACCTTGAAATTTCTCAGTGAGAACAAATGCCCTTTGGATGTGAAAGACAAGT
  CTGGAGAGATGGCCCTCCACGTGGCAGCTCGCTATGGCCATGCTGACGTGGCTCAGTTACTGTGCAGCTT
  CGGCTCAAATCCCAATATCCAGGACAAGGAAGAAGAAACCCCCCTGCACTGTGCTGCTTGGCACGGCTAT
  TACTCTGTGGCCAAAGCCCTTTGTGAAGCCGGCTGTAACGTGAACATCAAGAACCGAGAAGGAGAGACGC
  CCCTCCTGACAGCCTCTGCCAGGGGCTACCACGACATCGTGGAGTGTCTGGCCGAACATGGAGCCGACCT
  TAATGCTTGCGACAAGGACGGACACATTGCCCTTCATCTGGCTGTAAGACGGTGTCAGATGGAGGTAATC
  AAGACTCTCCTCAGCCAAGGGTGTTTCGTCGATTATCAAGACAGGCACGGCAATACTCCCCTCCATGTGG
  CATGTAAAGATGGCAACATGCCTATCGTGGTGGCCCTCTGTGAAGCAAACTGCAATTTGGACATCTCCAA
  CAAGTATGGGCGAACGCCTCTGCACCTTGCGGCCAACAACGGAATCCTAGACGTGGTCCGGTATCTCTGT
  CTGATGGGAGCCAGCGTTGAGGCGCTGACCACGGACGGAAAGACGGCAGAAGATCTTGCTAGATCGGAAC
  AGCACGAGCACGTAGCAGGTCTCCTTGCAAGACTTCGAAAGGATACGCACCGAGGACTCTTCATCCAGCA
  GCTCCGACCCACACAGAACCTGCAGCCAAGAATTAAGCTCAAGCTGTTTGGCCACTCGGGATCCGGGAAA
  ACCACCCTTGTAGAATCTCTCAAGTGTGGGCTGCTGAGGAGCTTTTTCAGAAGGCGTCGGCCCAGACTGT
  CTTCCACCAACTCCAGCAGGTTCCCACCTTCACCCCTGGCTTCTAAGCCCACAGTCTCAGTGAGCATCAA
  CAACCTGTACCCAGGCTGCGAGAACGTGAGTGTGAGGAGCCGCAGCATGATGTTCGAGCCGGGTCTTACC
  AAAGGGATGCTGGAGGTGTTTGTGGCCCCGACCCACCACCCGCACTGCTCGGCCGATGACCAGTCCACCA
  AGGCCATCGACATCCAGAACGCTTATTTGAATGGAGTTGGCGATTTCAGCGTGTGGGAGTTCTCTGGAAA
  TCCTGTGTATTTCTGCTGTTATGACTATTTTGCTGCAAATGATCCCACGTCAATCCATGTTGTTGTCTTT
  AGTCTAGAAGAGCCCTATGAGATCCAGCTGAACCAAGTGATTTTCTGGCTCAGTTTCCTGAAGTCCCTTG
  TCCCAGTTGAAGAACCCATAGCCTTCGGTGGCAAGCTGAAGAACCCACTCCAAGTTGTCCTGGTGGCCAC
  CCACGCTGACATCATGAATGTTCCTCGACCGGCTGGAGGCGAGTTTGGATATGACAAAGACACATCGTTG
  CTGAAAGAGATTAGGAACAGGTTTGGAAATGATCTTCACATTTCAAATAAGCTGTTTGTTCTGGATGCTG
  GGGCTTCTGGGTCAAAGGACATGAAGGTACTTCGAAATCATCTGCAAGAAATACGAAGCCAGATTGTTTC
  GGTCTGTCCTCCCATGACTCACCTGTGTGAGAAAATCATCTCCACGCTGCCTTCCTGGAGGAAGCTCAAT
  GGACCCAACCAGCTGATGTCGCTGCAGCAGTTTGTGTACGACGTGCAGGACCAGCTGAACCCCCTGGCCA
  GCGAGGAGGACCTCAGGCGCATTGCTCAGCAGCTCCACAGCACAGGCGAGATCAACATCATGCAAAGTGA
  AACAGTTCAGGACGTGCTGCTCCTGGACCCCCGCTGGCTCTGCACAAACGTCCTGGGGAAGTTGCTGTCC
  GTGGAGACCCCACGGGCGCTGCACCACTACCGGGGCCGCTACACCGTGGAGGACATCCAGCGCCTGGTGC
  CCGACAGCGACGTGGAGGAGCTGCTGCAGATCCTCGATGCCATGGACATCTGCGCCCGGGACCTGAGCAG
  CGGGACCATGGTGGACGTCCCAGCCCTGATCAAGACAGACAACCTGCACCGCTCCTGGGCTGATGAGGAG
  GACGAGGTGATGGTGTATGGTGGCGTGCGCATCGTGCCCGTGGAACACCTCACCCCCTTCCCATGTGGCA
  TCTTTCACAAGGTCCAGGTGAACCTGTGCCGGTGGATCCACCAGCAAAGCACAGAGGGCGACGCGGACAT
  CCGCCTGTGGGTGAATGGCTGCAAGCTGGCCAACCGTGGGGCCGAGCTGCTGGTGCTGCTGGTCAACCAC
  GGCCAGGGCATTGAGGTCCAGGTCCGCGGCCTGGAGACGGAGAAGATCAAGTGCTGCCTGCTGCTGGACT
  CGGTGTGCAGCACCATTGAGAACGTCATGGCCACCACGCTGCCAGGGCTCCTGACCGTGAAGCATTACCT
  GAGCCCCCAGCAGCTGCGGGAGCACCATGAGCCCGTCATGATCTACCAGCCACGGGACTTCTTCCGGGCA
  CAGACTCTGAAGGAAACCTCACTGACCAACACCATGGGGGGGTACAAGGAAAGCTTCAGCAGCATCATGT
  GCTTCGGGTGTCACGACGTCTACTCACAGGCCAGCCTCGGCATGGACATCCATGCATCAGACCTGAACCT
  CCTCACTCGGAGGAAACTGAGTCGCCTGCTGGACCCGCCCGACCCCCTGGGGAAGGACTGGTGCCTTCTC
  GCCATGAACTTAGGCCTCCCTGACCTCGTGGCAAAGTACAACACCAGTAACGGGGCTCCCAAGGATTTCC
  TCCCCAGCCCCCTCCACGCCCTGCTGCGGGAATGGACCACCTACCCTGAGAGCACAGTGGGCACCCTCAT
  GTCCAAACTGAGGGAGCTGGGTCGCCGGGATGCCGCAGACTTTTTGCTGAAGGCATCCTCTGTGTTCAAA
  ATCAACCTGGATGGCAATGGCCAGGAGGCCTATGCCTCGAGCTGCAACAGCGGCACCTCTTACAATTCCA
  TTAGCTCTGTTGTATCCCGGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001288731
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001288731.1, NP_001275660.1
• RefSeq Size: 5760 bp
• RefSeq ORF: 4293 bp
• Locus ID: 1612
• Cytogenetics: 9q21.33
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Bladder cancer, Pathways in cancer
• Gene Summary: 'Death-associated protein kinase 1 is a positive mediator of gamma-interferon induced programmed cell death. DAPK1 encodes a structurally unique 160-kD calmodulin dependent serine-threonine kinase that carries 8 ankyrin repeats and 2 putative P-loop consensus sites. It is a tumor suppressor candidate. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2013]'
  Transcript Variant: This variant (4) differs in the 5' UTR compared to variant 1. Variants 1, 2, 3 and 4 encode the same protein. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.