M2 (NC_004148) Virus Tagged ORF Clone

CAT#: VC101758

Myc-DDK-tagged ORF clone of viral ORF for matrix protein M2-2 [Human metapneumovirus], codon optimized for human cell expression, YP_012610

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101758 represents NCBI reference of YP_012610 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCTCCACATGCCGTGCAAAACAGTGAAGGCATTGATCAAGTGTAGTGAACACGGCCCCGTTTTCA
  TTACTATAGAGGTTGATGAGATGATCTGGACGCAGAAGGAACTGAAAGAGGCTCTGTCAGACGGCATTGT
  GAAGAGCCACACTAATATATATAATTGCTACTTGGAGAATATCGAGATCATCTACGTAAAGGCTTACCTG
  TCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101758 representing YP_012610
  Red=Cloning sites Green=Tags
  
  MTLHMPCKTVKALIKCSEHGPVFITIEVDEMIWTQKELKEALSDGIVKSHTNIYNCYLENIEIIYVKAYL
  S
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004148
• ORF Size: 213 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004148.2, YP_012610
• RefSeq ORF: 213
• Locus ID: 2799940
• MW: 8.2 kDa