N (NC_004148) Virus Tagged ORF Clone

CAT#: VC101753

Myc-DDK-tagged ORF clone of viral ORF for nucleoprotein [Human metapneumovirus], codon optimized for human cell expression, YP_012605

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: N
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101753 represents NCBI reference of YP_012605 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACTGCAGGGAATCCACTTGTCTGACCTTAGTTACAAGCATGCGATTCTGAAGGAATCACAGTACA
  CTATCAAGAGAGACGTGGGAACGACGACAGCTGTGACACCTAGCTCCTTGCAACAGGAAATCACCCTTTT
  GTGCGGGGAAATTCTGTACGCGAAACATGCAGACTATAAGTACGCGGCCGAAATCGGTATTCAGTATATA
  TCCACCGCACTCGGATCCGAGCGCGTGCAGCAGATACTCCGAAACTCTGGCTCAGAGGTGCAGGTGGTAC
  TTACTAGGACCTATTCTCTGGGAAAGGTGAAGAACAACAAAGGCGAGGATCTCCAGATGCTCGACATTCA
  TGGCGTTGAAAAAAGTTGGGTGGAGGAAATCGATAAGGAGGCGAGGAAGACAATGGCCACTCTGCTGAAA
  GAGTCTAGTGGGAACATCCCCCAGAATCAGCGCCCCTCCGCACCCGACACACCAATCATCCTCCTCTGCG
  TGGGCGCCCTGATTTTTACGAAACTCGCCTCCACTATAGAAGTGGGGCTCGAAACTACGGTACGGAGAGC
  CAATAGGGTGCTGTCCGATGCCCTGAAACGATACCCAAGAATGGACATACCTAAAATTGCCAGGAGCTTC
  TACGATCTGTTCGAACAGAAAGTGTATTACCGATCATTGTTTATCGAATACGGGAAGGCCCTGGGTTCCA
  GTAGTACAGGTTCCAAGGCGGAGTCCCTGTTCGTTAATATCTTCATGCAGGCTTACGGGGCTGGGCAGAC
  AATGCTCCGGTGGGGAGTGATCGCCAGAAGTAGTAATAACATCATGTTGGGGCATGTGAGCGTGCAGGCG
  GAGCTTAAACAAGTCACTGAAGTGTATGATTTGGTGCGGGAGATGGGGCCAGAGTCAGGATTGTTGCACC
  TGAGGCAGTCCCCTAAGGCTGGTCTGCTCTCCCTCGCTAATTGCCCCAATTTCGCCTCAGTCGTTCTGGG
  CAATGCCTCCGGTCTGGGCATCATCGGAATGTATAGGGGGCGGGTGCCGAATACGGAACTTTTTAGCGCT
  GCGGAGAGCTATGCGAAAAGCCTGAAAGAGTCTAACAAGATCAATTTTAGTTCTCTGGGGCTCACTGATG
  AAGAGAAAGAGGCCGCCGAGCATTTTTTGAACGTTAGCGATGACAGTCAGAATGATTATGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101753 representing YP_012605
  Red=Cloning sites Green=Tags
  
  MSLQGIHLSDLSYKHAILKESQYTIKRDVGTTTAVTPSSLQQEITLLCGEILYAKHADYKYAAEIGIQYI
  STALGSERVQQILRNSGSEVQVVLTRTYSLGKVKNNKGEDLQMLDIHGVEKSWVEEIDKEARKTMATLLK
  ESSGNIPQNQRPSAPDTPIILLCVGALIFTKLASTIEVGLETTVRRANRVLSDALKRYPRMDIPKIARSF
  YDLFEQKVYYRSLFIEYGKALGSSSTGSKAESLFVNIFMQAYGAGQTMLRWGVIARSSNNIMLGHVSVQA
  ELKQVTEVYDLVREMGPESGLLHLRQSPKAGLLSLANCPNFASVVLGNASGLGIIGMYRGRVPNTELFSA
  AESYAKSLKESNKINFSSLGLTDEEKEAAEHFLNVSDDSQNDYE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004148
• ORF Size: 1182 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004148.2, YP_012605
• RefSeq ORF: 1182
• Locus ID: 2799936
• MW: 43.5 kDa