E6 (NC_001694) Virus Tagged ORF Clone

CAT#: VC101825

Myc-DDK-tagged ORF clone of viral ORF for transforming protein E6 [Alphapapillomavirus 3], codon optimized for human cell expression, NP_043444

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101825 represents NCBI reference of NP_043444 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGACCATGTAATCCGACCAACATCTTTCTCTTGTGCAAAGATTATGAAGTCGATTTCGAGGACCTGA
  GACTCACATGCGTGTTCTGCAAGAACGAACTGACCACGGAGGAACTGCTGGCCTTCGCCCTCAAGGAGTT
  GTCCATAGTGTGGCGACACAACTGGCCTTTCGGGGTGTGCGCACCATGTCTGGCAAGAGAGGTCAAGGTT
  CGCGAACTGCGGCACTGGGATCATAGCTGTTACGGTCCAACCGTGGAGCAAACGACCGGCAGGTCTCTTG
  CCGAGTTGTACATTCGCTGCCATGCATGTAGCAAGCCCCTGAGTATACAGGAAAAGGAGCATCAGGTCCA
  GGCATACATTCATTTCCACTACATCGCGGGACAGTGGACTGGCAGATGTTGCCAGTGTCGCGGCCCTTGC
  ACCGCGCGGTGGCAACCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101825 representing NP_043444
  Red=Cloning sites Green=Tags
  
  MGPCNPTNIFLLCKDYEVDFEDLRLTCVFCKNELTTEELLAFALKELSIVWRHNWPFGVCAPCLAREVKV
  RELRHWDHSCYGPTVEQTTGRSLAELYIRCHACSKPLSIQEKEHQVQAYIHFHYIAGQWTGRCCQCRGPC
  TARWQP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001694
• ORF Size: 438 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001694.1, NP_043444
• RefSeq ORF: 438
• Locus ID: 1403312
• MW: 17.0 kDa