E7 (NC_001694) Virus Tagged ORF Clone

CAT#: VC101826

Myc-DDK-tagged ORF clone of viral ORF for transforming protein E7 [Alphapapillomavirus 3], codon optimized for human cell expression, NP_043445

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101826 represents NCBI reference of NP_043445 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGGTCAGGTTGCAACAATTAAGGACATAGTGCTGGAGGAGCGACCAGAAGTTGTAGACCTCCATT
  GCAACGAGCAACTGTTGGACAGCTCTGAAAGTGAGGAAGAGGATTCTGTAAGGGAACAGCTTGTTGAGCA
  AGCCCAGCAGGCCTACAGGGTAGTAACCACATGTGGGATCTGCAAGTGCCCTGTACGCCTGGTTGTGCAG
  TGTGGTGATGCAGACTTGAAGGTTCTGCATGAACTTCTGTTGGGTGACTTGTCCATAGTATGTCCGGGAT
  GTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101826 representing NP_043445
  Red=Cloning sites Green=Tags
  
  MHGQVATIKDIVLEERPEVVDLHCNEQLLDSSESEEEDSVREQLVEQAQQAYRVVTTCGICKCPVRLVVQ
  CGDADLKVLHELLLGDLSIVCPGCA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001694
• ORF Size: 285 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001694.1, NP_043445
• RefSeq ORF: 285
• Locus ID: 1403313
• MW: 10.5 kDa