E6 (NC_001576) Virus Tagged ORF Clone

CAT#: VC101832

Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041741

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E6
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101832 represents NCBI reference of NP_041741 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTATGGGAGCACAGGAGCCTCGCAATATTCTTCTCCTCTGCAGAAACTGTGGGATCCCCCTGGAGG
  ATCTGCGGCTCTGTTGCATCTTTTGTACGAAGCAGCTCACCGCCGCAGAGCTGGCAGCTTTCGCACTCCG
  GGAACTTTACCTTGTCTGGCGCGCGGGGGTGCCATATGGAGCCTGTGCAAGATGCTTGCTCCTGCAGGGA
  ATTGTGAGGCGGTTGAAGTACTGGGACTATAGTTACTACGTGGAGGGAGTCGAAGAAGAGACTAAGCAGT
  CAATCTATACACAGCTGATACGGTGTTATATGTGTCACAAGCCATTGGTGAGGGAGGAGAAGGACCGCCA
  CCGAAACGAGAGACGCCGCCTGCACAAGATCTCCGGATATTGGAGGGGTAGCTGCGAGTATTGCTGGAGC
  AGGTGCACAGTGAGAATACCCCAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101832 representing NP_041741
  Red=Cloning sites Green=Tags
  
  MSMGAQEPRNILLLCRNCGIPLEDLRLCCIFCTKQLTAAELAAFALRELYLVWRAGVPYGACARCLLLQG
  IVRRLKYWDYSYYVEGVEEETKQSIYTQLIRCYMCHKPLVREEKDRHRNERRRLHKISGYWRGSCEYCWS
  RCTVRIPQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001576
• ORF Size: 444 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001576.1, NP_041741
• RefSeq ORF: 444
• Locus ID: 1489375
• MW: 17.6 kDa