E7 (NC_001576) Virus Tagged ORF Clone

CAT#: VC101833

Myc-DDK-tagged ORF clone of viral ORF for envelope protein [Human papillomavirus type 10], codon optimized for human cell expression, NP_041742

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101833 represents NCBI reference of NP_041742 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCACGGCCCTCACCCGACCGTGAAGGACATCGAGCTGTCCCTCGCACCAGAGGATATACCCGTGTGCA
  ACGTGCAACTGGATGAGGAGGACTATACCGACGCTGTCGAACCCGCTCAGCAGGCCTACAGAGTGGTAAC
  TGAGTGCACTAAGTGTTCTCTGCCCCTCAGACTGGTGGTGGAATGCTCCCATGCTGACATACGCGCCCTT
  GAGCAATTGCTGCTCGGTACTCTCAAGCTGGTGTGTCCTAGATGTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101833 representing NP_041742
  Red=Cloning sites Green=Tags
  
  MHGPHPTVKDIELSLAPEDIPVCNVQLDEEDYTDAVEPAQQAYRVVTECTKCSLPLRLVVECSHADIRAL
  EQLLLGTLKLVCPRCV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001576
• ORF Size: 258 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001576.1, NP_041742
• RefSeq ORF: 258
• Locus ID: 1489376
• MW: 9.5 kDa