E1 (NC_012213) Virus Tagged ORF Clone

CAT#: VC101846

Myc-DDK-tagged ORF clone of viral ORF for early protein E1 [Human papillomavirus type 108], codon optimized for human cell expression, YP_002647035

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101846 represents NCBI reference of YP_002647035 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGCTGGCCACGCACTAAGGAGTCTGAGGGCCTTTCCAACAGCTGCGTCGAAACTCTTACCCTCTGTG
  TGCTTCCAGTGCCCAGCAATGAGAATGAGATGGAGGACAAGGATACAAAGGGTACGTCCTTCTGTATCGA
  TAACACTACCGAAGCCATTCAGTTCATCGAAACCGAAGCAGAATGCGACGAATGTCTTGATAGTCTGGAG
  GCACTGTTCGAGGAATCAGACGGGACTGATGTGTCCGACCTCATCAACAATATCGACGAGCCCGACGAGG
  GAAACCCCCACGCCCTCCTGAACAGGCAGCAGCTGGAGGAGGATTCACAACTGCTGAGCGTGCTGAAGAG
  GAAATATACCTCCCCCTCCCCCAAGCAGCAGGTGCTCGACCTTTCCCCCAGGCTGGAGTCACTCTGTGTG
  TCCCCAAGGGGCACCTCTTCAAAGCGAAGGCTTTTTGAAGGTTCAGGGCTTGGGCACGAAGCAGAGGACT
  CTAACGAAAGAGTGGAGAAGGTCGCGTCCCAGGCCGCACAGCCTAGCAACGAAACGAACGGCGAGCAGGA
  ACAGCAAAGTGTTTCAGCCACACAGAATGGCGACACCCAGTGCCTTGATATTCTTCACTCAAACAACCGG
  TTGGCCACCGCCCTGACAAAATTCAAGAATACCTTTGGAGTGGGATACAAGGAACTCACCAGGCCCTTCA
  AATCAAACAAGACTTGCTGCCACTCTTGGGTGGCTGTGATCTATGGGGTGCGAGCGGAAATGCTCGAGGC
  TAGCAAGACTCTGTTGAAGCCCCATTGTGACTTCTTTCAAATCCTGAACCCGAGCATGGGAACAGGAGTG
  ACCGTGCTGATCCTGTTTGAATTTGCCAGCTCAAAGTGTAGGGACACCGTCACAAGGTTGCTCTGTAATA
  TCTTCAACGTTGAAGAGTACCAAATCCTCGCAGATCCACCTAGACACAAAAGCGTCGCCGTCGCCCTGTT
  TTTTTACAGGCACTCACTGTCCAACATAAGCTTCACCGAAGGTGTCATGCCAGATTGGCTCAAGAAGCAA
  ACTATGCTGAATCATCAGCAGGAAGCGGACACGTTTGAACTGGCACCAATGGTCCAATGGGCCTACGACA
  ATAACATCTGGGAGGAAGCCGAGCTGGCCTATCAGTATGCGTGCTTGGCAGACGTCGAACCTAACGCAGC
  TGCTTGGCTGAAGTCCAATAACCAGTACAAGTATGTGTCCGATTGTGCAAAAATGGTAAAGATGTATAAG
  CGGTACGAGATGAGACAGATGAGTATAGGCCAGTGGATCAAGAAGTGCGGCGAGACGGTCGATGGCGAGG
  GAGATTGGAAAAAACTGATCAATCTCCTGAAGTACCAGGACGTCTCAATCATTCCATTCCTGACCAGCCT
  GAGGCTGTTCTTGAAAGGCGAGCCGAAAAAGAACTGCATCGTGATCTGGGGTCCTCCTGACACCGGGAAA
  TCCATGTTCTGCTACTCCTTGATTCGGTATCTTCAGGGGAAGGTGGTCTCATATGCCAACTCTAAAAGTC
  AGTTCTGGCTCCAGCCTCTTACGGAGGCCAAAATTGGTCTGATCGATGACGCAACGTTTCCATGTTTCCA
  GTTCATGGATGTATACATGAGGTCCGGACTCGATGGGAACGAAGTCTCCGTTGATTGTAAACATAAGACC
  CCCATGCAAGTAAAGTTGCCGCCTATGTTGGTGACCAGTAATATCAATGTTCACGCAGAGACTTCCCTGA
  AGTACCTGCACAGCAGACTCACCGGATACAATTTTCCCCATAAGCTCCCTGTAGACTCTAACGGAAACGT
  GGTGTATAAGATTACACACGCAGATTGGAAATGCTTTCTCGCCTCATGGTCCTCAAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101846 representing YP_002647035
  Red=Cloning sites Green=Tags
  
  MCWPRTKESEGLSNSCVETLTLCVLPVPSNENEMEDKDTKGTSFCIDNTTEAIQFIETEAECDECLDSLE
  ALFEESDGTDVSDLINNIDEPDEGNPHALLNRQQLEEDSQLLSVLKRKYTSPSPKQQVLDLSPRLESLCV
  SPRGTSSKRRLFEGSGLGHEAEDSNERVEKVASQAAQPSNETNGEQEQQSVSATQNGDTQCLDILHSNNR
  LATALTKFKNTFGVGYKELTRPFKSNKTCCHSWVAVIYGVRAEMLEASKTLLKPHCDFFQILNPSMGTGV
  TVLILFEFASSKCRDTVTRLLCNIFNVEEYQILADPPRHKSVAVALFFYRHSLSNISFTEGVMPDWLKKQ
  TMLNHQQEADTFELAPMVQWAYDNNIWEEAELAYQYACLADVEPNAAAWLKSNNQYKYVSDCAKMVKMYK
  RYEMRQMSIGQWIKKCGETVDGEGDWKKLINLLKYQDVSIIPFLTSLRLFLKGEPKKNCIVIWGPPDTGK
  SMFCYSLIRYLQGKVVSYANSKSQFWLQPLTEAKIGLIDDATFPCFQFMDVYMRSGLDGNEVSVDCKHKT
  PMQVKLPPMLVTSNINVHAETSLKYLHSRLTGYNFPHKLPVDSNGNVVYKITHADWKCFLASWSSS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012213
• ORF Size: 1878 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012213.1, YP_002647035
• RefSeq ORF: 1878
• Locus ID: 7559098
• MW: 70.8 kDa