L2 (NC_012213) Virus Tagged ORF Clone

CAT#: VC101848

Myc-DDK-tagged ORF clone of viral ORF for late gene L2 [Human papillomavirus type 108], codon optimized for human cell expression, YP_002647037

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101848 represents NCBI reference of YP_002647037 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACGCCCAGCCAAGCGCGAAGCGACGGAAACGGGACACCGTTGAGAATATCTATCGCCACTGTGTGG
  GGTCAGGCACGTGTCCTGATGATGTGAGAAATAAAGTCGAACAGACTACCCTGGCTGACAAGATACTGAA
  GTGGATCGCATCCGTAGTATACTTCGGCGGCCTCGGGATCGGCACCGGTAAAGGAACAGGCGGCAGCAGT
  GGTTACGTGCCAATTGGAACTGGCGGTGGTGGCAGAGTGACATCACAGGGTACACCGCTGAGACCCTCTG
  TTGTCGTTGACCCTATCGGCCCTCCAGATATTGTTCCCGTAGATGTCGTTACCCCAGACACGGCGTCTAT
  AGTGCCTCTGGAAGTCCCTCCCACACCGACGGACGCAATCGTTCCAGTGGATCCTCTTCAGCCTGATATA
  GTGGTACCACTGGATCCTGTGTCCGATATTACAATCGATACTACAAGCCCTGCTGTCGCTACGATTGACT
  CAACATCCGCTGTCCTGGAAGTACAACCAACCACTTCCACCCCTAAAAGGGTGTCCCTTAATTCAAGCCG
  ACACGCTACCCCCTCTTATATTTCCGTGTACGGGCATCCAACTGACCCGGTAAGTCAGGCGGCAGCGGAG
  GTGATTATCGGCGCTGCTGCAACGGACGGAACCGTTGTCGACATCGGCGAGTCCATCCCTTTGGACACAT
  TCGTGCAGTCTCGGGGGTCCAGTACCTTCGATATTGAGCCAGCTGCACAGCCTCGAACCTCTACACCTCG
  GGCCTTTCAGAGAGCCTTTCAGCGGGCGAGAGACCTCTACAATCGCAGGGTACAACAAGTGAGGACCAGG
  AATGTTAACTTCCTGAACAGACCACGGCAGGCTGTCACCTTTCAGTTTGAAAACCCAGCTTTTGAAGAAG
  AAGTCACACTGCAGTTCGAACAGGACCTGAACACTCTGGCCGCAGCTGCTCCAGATCCTGACTTTGCCGA
  CGTGATCCGCTTGGGCAGGCCCAGAGTTAGCGAAACGCCTGAGGGACACGTTCGAATATCAAGACTCGGA
  CAGAGGGGTACCATCCGGACTAGGTCTGGGCTGCAGATCGGCCCTCACGTACACTACTATTATGATATAT
  CATCAATTCATCCTGACAGCATCGAACTGCGCCCCATAGGACAGCATAGTGGCGAGTCCGGTATCATATC
  TAATGCTTCCGCCGTGGCCGAATCTAGTTTTATTGAGGGGCACACTATTCAGGAGCCACTCCTCAATGCC
  GACCTGGAGGATACTTATGCCGAGGACTTTACTAGCAGCCGACTCAGATTCGCAGGTCTCCAGGGAGTCT
  CCATCTCTCCCGCCCTCGAAACCGTGGCACTGCCTCCCGGGGCCGCCCTGCAGTTCATCATCACTAGCAC
  TGGCGGAAAACGCCCCACAACATTTCCCATACCCCAACCAGTTCCCATGCAGCCATATGTCCCGTTTGTG
  ATTGTGGATTCATTCATCAGCAGTGATACGTTTTACCTGCATCCTAGCCTCGTTCGAAAGCGGAAGCGAA
  GAGACCTGGTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101848 representing YP_002647037
  Red=Cloning sites Green=Tags
  
  MDAQPSAKRRKRDTVENIYRHCVGSGTCPDDVRNKVEQTTLADKILKWIASVVYFGGLGIGTGKGTGGSS
  GYVPIGTGGGGRVTSQGTPLRPSVVVDPIGPPDIVPVDVVTPDTASIVPLEVPPTPTDAIVPVDPLQPDI
  VVPLDPVSDITIDTTSPAVATIDSTSAVLEVQPTTSTPKRVSLNSSRHATPSYISVYGHPTDPVSQAAAE
  VIIGAAATDGTVVDIGESIPLDTFVQSRGSSTFDIEPAAQPRTSTPRAFQRAFQRARDLYNRRVQQVRTR
  NVNFLNRPRQAVTFQFENPAFEEEVTLQFEQDLNTLAAAAPDPDFADVIRLGRPRVSETPEGHVRISRLG
  QRGTIRTRSGLQIGPHVHYYYDISSIHPDSIELRPIGQHSGESGIISNASAVAESSFIEGHTIQEPLLNA
  DLEDTYAEDFTSSRLRFAGLQGVSISPALETVALPPGAALQFIITSTGGKRPTTFPIPQPVPMQPYVPFV
  IVDSFISSDTFYLHPSLVRKRKRRDLV
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_012213
• ORF Size: 1551 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_012213.1, YP_002647037
• RefSeq ORF: 1551
• Locus ID: 7559097
• MW: 55.7 kDa