L2 (NC_014956) Virus Tagged ORF Clone

CAT#: VC101891

Myc-DDK-tagged ORF clone of viral ORF for L2 [Human papillomavirus type 134], codon optimized for human cell expression, YP_004169296

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: L2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101891 represents NCBI reference of YP_004169296 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGAACGCCCACGCAAAAGGACAAAAAGAGACTCCGTGACCAATCTGTATAACCAGTGCAAAATCA
  GTGGATCATGTCCGGATGACGTAAAGAATAAAGTAGAGGAAACCACCCTGGCCGATCGGCTTCTCAAGAT
  CTTCAGCAGCCTTGTCTACTTTGGTGGACTGGGCATTGGCACAGGCAAGGGCATCGGGGGATCAACAGGC
  TATCGCCCGCTCGGGGGCGGAAGCGGCAGGGTGACCGCAGGAGGAACAGTAATTAGGCCCAATGTTGCTG
  TGGACCCGCTCGGTCCTACCGATATTGTCCCCATTGACTCTATCAGCCCCGGTACAAGCAGTATTGTGCC
  TCTCATTGAGGTGACTCCCGAGGTGGTACCTGAGCTGGAGGCTGGTGTGATTAACACGGGTTTTGATATC
  ACTACCGAACCTGACATTATCGAAATCAGCTCTGGCTCTAATACCCCCGCTGTATCAACCATTGACGATA
  CAGCCGCTGTCCTGGAGGTGCAGCCCAGCACAACCACTCCGCGAAGAGTTACTACTAGCAAGTTCACCAA
  TCCGACATACATGACTGTGGTTACAGAAAGCACAATCACCCCAGAAGTTTCCTCAACAGCTGGCGTTTTC
  ATTGACGGCGCACTCGGCGGTGACGTCGTAGGCGAGAACATTCCTCTTGATACATTCAATGAGCCCTTGG
  AGTTCGAGATTCTGGAGGAGCAGCAGCCTAAGACTTCCACACCCATTGAAAATGTGACGCGAGCCCTGGG
  CCGCGCGCGAGAGCTGTACAACAGACGGGTGCGGCAGGTGATGACTAGGAACCCTAATTTCCTGACCCGG
  GCACCTCAAGCTGTCCAATTTGTCTTTGAGAACCCCGCATTCACCAACGATGTGACCCTGACTTTCGAGC
  AGGATCTCAATCAGATCGCCACCGCAGCACCAGATCCTGACTTTGCAGATATAATCAAGCTGGAAAGGCC
  ACGCTTTTCTGAGACCGCCGAAGGCACAGTTAGGTTGAGCAGGCTTGGGACACGCGGTACCATAAGGACG
  AGGTCCGGCACCCAGATAGGCGAGCGCGTGCACTTCTACTACGACCTGTCCAAAATCGAGAACAGCGAAG
  CCATCGAGCTGTCCGTCCTGGGTGAGCACTCAGGAGACGCCACCTTTATCAACCCGCTGGCGGAATCAAC
  CTTTGTCGACGCCGAGAACACAAACATGCCCGCTATCTTCCCCGAAGAAGAACTGGTGGATGAAATAACA
  GAAGACTTCTCAAACTCCCACGTGGTCCTGATTAATGGCTCCAGAAGAAGCACAATGAACGTTCCCACGC
  TTCCTCCCGGCGTTGCATTGCGCGTTTACGTGGATGATTACGGAGAAGGACTCTTCGTCAGTCACCCACA
  GGGACGCGAGCTGCCCGCTATCATTACCCCTGGCAACGCTGGCCGACCTTCTATTCTCATTGATGATTTT
  GCCTCCAACGATTTTGTCCTGCACCCATCCCTGAATCGCAAGAAGAGAAAGCGAAAGCAGGTTCACTTCC
  TT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101891 representing YP_004169296
  Red=Cloning sites Green=Tags
  
  MSERPRKRTKRDSVTNLYNQCKISGSCPDDVKNKVEETTLADRLLKIFSSLVYFGGLGIGTGKGIGGSTG
  YRPLGGGSGRVTAGGTVIRPNVAVDPLGPTDIVPIDSISPGTSSIVPLIEVTPEVVPELEAGVINTGFDI
  TTEPDIIEISSGSNTPAVSTIDDTAAVLEVQPSTTTPRRVTTSKFTNPTYMTVVTESTITPEVSSTAGVF
  IDGALGGDVVGENIPLDTFNEPLEFEILEEQQPKTSTPIENVTRALGRARELYNRRVRQVMTRNPNFLTR
  APQAVQFVFENPAFTNDVTLTFEQDLNQIATAAPDPDFADIIKLERPRFSETAEGTVRLSRLGTRGTIRT
  RSGTQIGERVHFYYDLSKIENSEAIELSVLGEHSGDATFINPLAESTFVDAENTNMPAIFPEEELVDEIT
  EDFSNSHVVLINGSRRSTMNVPTLPPGVALRVYVDDYGEGLFVSHPQGRELPAIITPGNAGRPSILIDDF
  ASNDFVLHPSLNRKKRKRKQVHFL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_014956
• ORF Size: 1542 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_014956.1, YP_004169296
• RefSeq ORF: 1542
• Locus ID: 10146753
• MW: 55.9 kDa