E2 (NC_001591) Virus Tagged ORF Clone

CAT#: VC101953

Myc-DDK-tagged ORF clone of viral ORF for E2 protein [Human papillomavirus type 49], codon optimized for human cell expression, NP_041835

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101953 represents NCBI reference of NP_041835 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGCCCTCAATGCCAGGTTTAATGTGCTGCAGGAGATGCTGATGGACATTTACGAATCCGGCAAGG
  AAGATCTGGAAACACAGATAGAACACTGGAAGCTGCTGCGGCAGGAACAGGCATTGCTCTTTTTCGCTCG
  CAAACACTCAATTATGCGCCTTGGGTACCAGCCAGTGCCGCCCATGGCGGTAAGCGAGACCAAAGCTAAG
  CAGGCGATTGGAATGATGCTGACACTGCAGTCCCTTCAGAAGTCCCCGTTCGGGAAGGAAAAATGGACAT
  TGGTCAATACCTCCCTGGAGACCTACAATGCTCCACCCGCCCAGTGCTTCAAGAAAGGGCCATATAACAT
  CGAAGTGATTTTCGATGGGGACCCCGAGAACCTGATGGTGTATACAGCCTGGAAAGAAATCTATTTCGTT
  GACTCTGATGATATGTGGCAGAAGGTTCAGGGAGAGGTCGACTACGCTGGAGCTTACTACAAGGACGGTA
  CAATTAAGCAGTACTATGTAACATTTGCTGACGACGCTGTCAGATATGGGACCAGCGGACAGTACGAAGT
  TCGCATCAATAATGAAACAGTGTTTGCACCCGTGACTTCAAGCACCCCCCCTTCCACAGGACTTCGGGAG
  AGCTCTAATGCTAGTCCTGTTCACGATACAGTGGATGAGACTCCAACTTCCACCACCGCCACAACCACTA
  CCTTTAGTACAACTACAGCTACCGCTACTGCGACAGGAGCTCCCGAGTTGAGTTCAAAAACCGGGACCCG
  GAAAGGCAGATACGGACGCAAGGATTCCTCACCTACGGCTGCATCAAATTCTCGAAAGGAAGTCAGTCGG
  AGACGGTCCCGGTCTCGGACCAGAACTAGGCGGCGAGAGGCTTCCACGAGTAGGTCCCAAAAGGCTTCCC
  GGTCTAGGTCCCGCTCCAGAAGTACCAGCCGGGGCTCCAGAGGGAGCGGGGGTAGCGTTACTACCAGTAG
  GGATTCTTCCCCCAAGCGCACTAGGAGAGGTCGGGGACGCGGGGGACGGTCAAGACGGTCTCCCACTCCT
  ACCTCAACCAGCAAACGCGAGCGGCGCAGATCACGAAGCCGGGGAGGGGAACCTGTGTCAGGCGGTGTCG
  GAATCTCTCCCGACAAGGTGGGCTCTCGAGTCCAGACCGTGTCAGGTCGCCACCTCGGGCGACTGGGCAG
  ATTGCTGGAGGAGGCCAGTGACCCTCCCGTGATTCTCCTGCGCGGAGATCCCAATATTTTGAAGTGCTAC
  CGGTATCGGGACAAAAAGCGGAAACTGGGATTGGTCAAGCATTACAGCACAACCTGGTCCTGGGTCGGCG
  TCGATGGGAACGAAAGAATCGGGCGCTCCAGAATGCTTCTGTCCTTCACCAGCAATTCTACCAGATCTCA
  GTACGTGAAAATTATGAAGCTGCCTAAGGGCGTCGAATGGTCTTTCGGGAACTTCGATAAACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101953 representing NP_041835
  Red=Cloning sites Green=Tags
  
  MEALNARFNVLQEMLMDIYESGKEDLETQIEHWKLLRQEQALLFFARKHSIMRLGYQPVPPMAVSETKAK
  QAIGMMLTLQSLQKSPFGKEKWTLVNTSLETYNAPPAQCFKKGPYNIEVIFDGDPENLMVYTAWKEIYFV
  DSDDMWQKVQGEVDYAGAYYKDGTIKQYYVTFADDAVRYGTSGQYEVRINNETVFAPVTSSTPPSTGLRE
  SSNASPVHDTVDETPTSTTATTTTFSTTTATATATGAPELSSKTGTRKGRYGRKDSSPTAASNSRKEVSR
  RRSRSRTRTRRREASTSRSQKASRSRSRSRSTSRGSRGSGGSVTTSRDSSPKRTRRGRGRGGRSRRSPTP
  TSTSKRERRRSRSRGGEPVSGGVGISPDKVGSRVQTVSGRHLGRLGRLLEEASDPPVILLRGDPNILKCY
  RYRDKKRKLGLVKHYSTTWSWVGVDGNERIGRSRMLLSFTSNSTRSQYVKIMKLPKGVEWSFGNFDKL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001591
• ORF Size: 1464 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001591.1, NP_041835
• RefSeq ORF: 1464
• Locus ID: 1489443
• MW: 54.7 kDa