E1 (NC_001531) Virus Tagged ORF Clone

CAT#: VC101958

Myc-DDK-tagged ORF clone of viral ORF for replication protein [Human papillomavirus type 5], codon optimized for human cell expression, NP_041367

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101958 represents NCBI reference of NP_041367 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTGACCCCAACAGCAAGGGATCTACATCCAAGGAAGGATTCGGCGACTGGTGCCTCCTTGAAGCAG
  ATTGCTCCGACGTCGAAAATGATCTGGGACAGCTGTTTGAGAGAGATACAGACTCCGATATCTCCGACCT
  CCTTGACGACACAGAGCTCGAGCAAGGAAATTCTCTTGAGCTCTTCCACCAACAAGAATGCGAACAGAGT
  GAGGAGCAGCTGCAGAAGCTCAAAAGGAAGTATCTCAGTCCAAAAGCAGTGGCTCAGCTGAGCCCTAGAT
  TGGAAAGCATTAGCTTGAGCCCTCAGCAGAAGTCTAAAAGGCGGCTCTTTGCAGAACAGGACTCTGGACT
  GGAGCTGACATTGAATAACGAAGCCGAGGACGTCACCCCTGAAGTGGAGGTGCCCGCAATCGATTCTCGG
  CCCGATGACGAAGGAGGCTCTGGGGACGTTGACATACACTATACCGCCCTGTTGCGAAGCTCAAATAAAA
  AAGCAACACTCATGGCCAAATTCAAGGAAAGCTTCGGAGTGGGGTTTAATGAGCTGACCCGCCAGTTCAA
  GTCTCACAAAACCTGTTGCAAAGACTGGGTGGTGTCCGTGTACGCCGTGCACGACGATCTTTTTGAATCC
  TCCAAGCAACTCCTGCAGCAGCATTGCGATTATATTTGGGTTAGAGGCATAGGTGCCATGTCCCTGTACC
  TGCTTTGTTTCAAGGCCGGGAAGAATCGGGGCACGGTCCACAAGCTGATTACCAGCATGCTGAACGTCCA
  CGAACAACAAATCCTGTCTGAGCCTCCAAAGCTGCGGAACACCGCCGCCGCCCTGTTCTGGTATAAGGGC
  TGCATGGGTAGTGGAGCCTTCTCCCATGGACCATACCCGGACTGGATCGCACAGCAAACCATCCTGGGAC
  ATAAATCCGCCGAAGCAAGCACATTCGACTTTTCTGCCATGGTGCAGTGGGCGTTCCATAATCACCTTCT
  CGACGAGGCCGACATTGCTTACCAATACGCCCGGCTGGCCCCCGAAGACGCAAACGCTGTGGCATGGCTG
  GCTCACAATAATCAGGCTAAGTTTGTGCGAGAATGTGCCTACATGGTCCGATTTTACAAAAAGGGCCAGA
  TGAGAGACATGTCTATCAGCGAATGGATTTACACTAAGATCAACGAGGTCGAGGGGGAAGGGCACTGGAG
  CGATATCGTCAAGTTCATTAGATATCAGAATATTAATTTTATAGTTTTTCTTACGGCTCTTAAAGAATTC
  CTCCACAGTGTCCCCAAAAAGAACTGCATCCTCATCTATGGGCCTCCCAATAGTGGAAAGTCCTCATTCG
  CCATGAGCCTTATCCGAGTCCTGAAGGGCAGGGTGCTGAGCTTCGTGAACTCCAAGTCTCAGTTCTGGCT
  GCAACCGCTGAGCGAGTGCAAGATCGCCCTCCTCGACGATGTGACTGACCCATGTTGGATTTACATGGAC
  ACTTATCTGAGGAACGGCCTGGATGGCCACTATGTGTCCCTGGACTGCAAGTACAGAGCGCCAACACAGA
  TGAAGTTTCCACCCCTGTTGCTGACAAGCAATATTAATGTGCACGGGGAAACAAATTATAGGTACTTGCA
  CACGACCATTAAAGGCTTCGAGTTCCCAAATCCTTTTCCTATGAAGGCAGATAACACGCCCCAATTCGAG
  TTGACAGATCAGAGCTGGAAATCCTTCTTCACTCGACTTTGGACTCAGCTCGATCTGTCCGACCAGGAAG
  AAGAAGGCGAAGATGGGGAGTCACAGCGCGCCTTCCAATGCAGCGCGCGCAGCGCAAATGAACATCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101958 representing NP_041367
  Red=Cloning sites Green=Tags
  
  MTDPNSKGSTSKEGFGDWCLLEADCSDVENDLGQLFERDTDSDISDLLDDTELEQGNSLELFHQQECEQS
  EEQLQKLKRKYLSPKAVAQLSPRLESISLSPQQKSKRRLFAEQDSGLELTLNNEAEDVTPEVEVPAIDSR
  PDDEGGSGDVDIHYTALLRSSNKKATLMAKFKESFGVGFNELTRQFKSHKTCCKDWVVSVYAVHDDLFES
  SKQLLQQHCDYIWVRGIGAMSLYLLCFKAGKNRGTVHKLITSMLNVHEQQILSEPPKLRNTAAALFWYKG
  CMGSGAFSHGPYPDWIAQQTILGHKSAEASTFDFSAMVQWAFHNHLLDEADIAYQYARLAPEDANAVAWL
  AHNNQAKFVRECAYMVRFYKKGQMRDMSISEWIYTKINEVEGEGHWSDIVKFIRYQNINFIVFLTALKEF
  LHSVPKKNCILIYGPPNSGKSSFAMSLIRVLKGRVLSFVNSKSQFWLQPLSECKIALLDDVTDPCWIYMD
  TYLRNGLDGHYVSLDCKYRAPTQMKFPPLLLTSNINVHGETNYRYLHTTIKGFEFPNPFPMKADNTPQFE
  LTDQSWKSFFTRLWTQLDLSDQEEEGEDGESQRAFQCSARSANEHL
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001531
• ORF Size: 1818 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001531.1, NP_041367
• RefSeq ORF: 1818
• Locus ID: 1489049
• MW: 69.1 kDa