E7 (NC_001691) Virus Tagged ORF Clone

CAT#: VC101965

Myc-DDK-tagged ORF clone of viral ORF for transforming protein E7 [Human papillomavirus type 50], codon optimized for human cell expression, NP_043424

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101965 represents NCBI reference of NP_043424 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGGTGATAAGCCAACTATCCCTGATATCGTTCTCGAAGAACTGGTTCTGCCGGTTAACCTGTTGA
  GTGATGAATCAATCGAAACGGATGATATAGCCGAGAGTGAGAGATCTCCCTTTAAAATAGATAGCACATG
  TAAGCACTGTCACTGCCGAGTGCGGCTCTGTTGCGTCGCTACCGATGCCGCGATCCGAGTGTTTGAGTGT
  CTGCTGCAGAGCGAGTTTAGTTTCCTTTGCCTTAAATGTTCTAAAGAACTTCTGCGGAATGGTCGGACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101965 representing NP_043424
  Red=Cloning sites Green=Tags
  
  MRGDKPTIPDIVLEELVLPVNLLSDESIETDDIAESERSPFKIDSTCKHCHCRVRLCCVATDAAIRVFEC
  LLQSEFSFLCLKCSKELLRNGRT
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001691
• ORF Size: 279 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001691.1, NP_043424
• RefSeq ORF: 279
• Locus ID: 1403630
• MW: 10.5 kDa