E4 (NC_001691) Virus Tagged ORF Clone

CAT#: VC101968

Myc-DDK-tagged ORF clone of viral ORF for putative, partial [Human papillomavirus type 50], codon optimized for human cell expression, NP_043427

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101968 represents NCBI reference of NP_043427 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  CTGGTGATACAATATACGCTTAACAGCTTTAACCTCATGCCTTTGTACATGGCCAATCTGGACAATGGGC
  TGCTGTACCTGAAGACAAAACTGTTTATCCCACTGAGCTTGGCTCCCCAGGGGGTCCGGCTTGGGCTGCT
  CACCAACTCTCCCGGACTGCCTCACAGGACCACGAGGGCGAACCGGAAGGATCTCGAAGCCGTTAACCAG
  AAGCCATATCGGACCCCTAACCATCCTCCTCGACACCAGCAGTACGACTTCGACGAGGACGACGAGAAAG
  AGAATACCATTCCAACAGATACAGAGTCTCACAATCAAAATTGGGAACCAACCCTGCGACAATTGCTTAA
  GAAATGGGAAGAGGACCTGACCCAATTGCAACGAATGGTGTGTCAGGATTTGGATGATTATAAGCGGAAA
  CTCGGGATTCACCAGTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101968 representing NP_043427
  Red=Cloning sites Green=Tags
  
  LVIQYTLNSFNLMPLYMANLDNGLLYLKTKLFIPLSLAPQGVRLGLLTNSPGLPHRTTRANRKDLEAVNQ
  KPYRTPNHPPRHQQYDFDEDDEKENTIPTDTESHNQNWEPTLRQLLKKWEEDLTQLQRMVCQDLDDYKRK
  LGIHQS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001691
• ORF Size: 438 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001691.1, NP_043427
• RefSeq ORF: 438
• Locus ID: 1403633
• MW: 17.2 kDa