hypothetical protein (NC_001458) Virus Tagged ORF Clone

CAT#: VC101989

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HpV63gp5 [Human papillomavirus type 63], codon optimized for human cell expression, NP_040900

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC101989 represents NCBI reference of NP_040900 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  AATATGATGGTGAGCAAAACCACTATGCTTACTTTCAAGAAGCGGCCTATCGACATCGCTAAGCAAGTTG
  ATATACTCTTCAACATGCGCGTGAAAGGATCCCAGATGCTTTGTCTTCTTTCAATAGCCCCCCACTACGG
  ACTGCTCGGCCTGTTGCAGACCCCCACACAGCCCCCAAAAGATAACCCCCCTAAACTGCCCGAGAAGCAA
  CGGAGACGGGGCAGGGATACAACCCGCAATCGGCGCCTCTTCGCCAGCGATGGACCAACAGATGAGGAGG
  GACCGGAAGTTCCAGAGATACCGCCCTCCGATGAGGAAAAAGAGAATCGCCCGGAGCCCCTCCCGGTCGT
  GGAAAACGGATGGCACTCCTTCCTCCGAGAGACACTGGAGCATCAGCTTGGCCGGCTTCAGCGGGAGGTG
  AATCAAGACTTCGAAGACCTCTACCGGAGGCTCGGTATCCACCCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC101989 representing NP_040900
  Red=Cloning sites Green=Tags
  
  NMMVSKTTMLTFKKRPIDIAKQVDILFNMRVKGSQMLCLLSIAPHYGLLGLLQTPTQPPKDNPPKLPEKQ
  RRRGRDTTRNRRLFASDGPTDEEGPEVPEIPPSDEEKENRPEPLPVVENGWHSFLRETLEHQLGRLQREV
  NQDFEDLYRRLGIHP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001458
• ORF Size: 465 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001458.1, NP_040900
• RefSeq ORF: 465
• Locus ID: 1494572
• MW: 18.0 kDa