E7 (NC_004104) Virus Tagged ORF Clone

CAT#: VC102022

Myc-DDK-tagged ORF clone of viral ORF for putative transforming protein E7 [Human papillomavirus type 90], codon optimized for human cell expression, NP_671504

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: E7
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102022 represents NCBI reference of NP_671504 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCATGGCCAGCGGCCCACTCTGAAGGACATTTTTCTCGAGCTTGACCAGCCCGACGCAGTCGATCTGC
  ACTGTAATGAGCAACTTGCCTCTAGTGAAGAAGAGGATAATAGAGAGGACGGCGAGCAGCCAACCCAGCC
  AACCGAACCAGCCCAGCAAGCCTATCGCGTAGTCACAAGCTGCTGTCGCTGCTGTTGCACCGTCCGCCTG
  GTGGTTGAGAGTGGCCACGCTGAAATCAGGCAATTGCAGGAACTGTTGCTGGGAGCGCTGCACATAGTCT
  GCCCTCTTTGCGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102022 representing NP_671504
  Red=Cloning sites Green=Tags
  
  MHGQRPTLKDIFLELDQPDAVDLHCNEQLASSEEEDNREDGEQPTQPTEPAQQAYRVVTSCCRCCCTVRL
  VVESGHAEIRQLQELLLGALHIVCPLCA
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_004104
• ORF Size: 294 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_004104.1, NP_671504
• RefSeq ORF: 294
• Locus ID: 951485
• MW: 10.9 kDa