hypothetical protein (NC_007026) Virus Tagged ORF Clone

CAT#: VC102087

Myc-DDK-tagged ORF clone of viral ORF for hypothetical protein HPV_s1gp2 [Human picobirnavirus], codon optimized for human cell expression, YP_239360

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: hypothetical protein
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102087 represents NCBI reference of YP_239360 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGCAGAACGATACCAAGAAAACTACTCAGCGGCGGAACAGCAAGAAGTATAGCAGCAAGACTAACC
  GGGGCACCAAGCGGGCACCTCGGGATCAGGAAGTCGGCACCGGCGCTCAAGAAAGCACCAGAAACGACGT
  GGCCTGGTATGCACGGTACCCTCACATTCTGGAGGAGGCCACCCGGCTGCCCTTCGCATACCCCATCGGG
  CAATACTACGACACCGGCTACAGCGTGGCAAGCGCCACCGAGTGGAGCAAGTACGTGGACACAAGCCTGA
  CCATTCCCGGAGTGATGTGCGTCAACTTCACCCCCACACCCGGGGAAAGCTATAACAAGAACAGCCCAAT
  CAACATCGCCGCCCAGAACGTCTACACCTACGTGCGGCACATGAACAGCGGCCACGCCAACTACGAACAG
  GCCGACCTCATGATGTATCTCCTGGCCATGGATAGCCTGTACATTTTCCACAGCTACGTGCGGAAGATCC
  TGGCTATCAGCAAGCTGTACACTCCCGTGAACAAGTACTTCCCTCGGGCACTGCTCGTGGCCCTCGGCGT
  CGACCCCGAGGACGTGTTTGCAAACCAGGCCCAGTGGGAGTACTTCGTGAACATGGTCGCCTATCGGGCC
  GGGGCCTTTGCCGCCCCTGCTTCCATGACCTACTACGAGCGGCACGCCTGGATGTCCAACGGCCTGTACG
  TCGACCAGGACGTGACCCGGGCCCAGATTTACATGTTCAAGCCCACCATGCTGTGGAAGTACGAGAACCT
  CGGCACCACCGGGACCAAGCTGGTCCCCCTGATGATGCCCAAGGCCGGCGACAACCGGAAGCTGGTCGAC
  TTTCAGGTGCTGTTTAACAACCTGGTGAGCACCATGCTGGGGGACGAGGACTTCGGGATCATGTCCGGAG
  ATGTGTTCAAGGCCTTCGGAGCCGACGGCCTCGTGAAGCTCCTGGCCGTCGACAGCACAACCATGACACT
  GCCCACCTACGACCCACTGATCCTGGCACAGATCCACTCCGCCCGGGCCGTGGGCGCCCCCATCCTCGAG
  ACCTCCACACTGACCGGGTTCCCCGGCCGGCAGTGGCAGATCACTCAAAACCCCGATGTCAACAACGGCG
  CCATCATCTTCCACCCCAGCTTCGGCTACGACGGGCAGGACCACGAGGAGCTGAGCTTCCGGGCCATGTG
  CTCCAACATGATCCTCAACCTGCCCGGCGAAGCCCATTCCGCCGAGATGATCATCGAGGCCACCCGGCTG
  GCCACCATGTTCCAGGTGAAGGCCGTGCCCGCTGGAGACACTAGCAAGCCCGTGCTCTACCTGCCCAACG
  GGTTTGGCACCGAGGTCGTGAACGACTACACAATGATCAGCGTGGACAAGGCCACTCCCCACGACCTCAC
  TATTCACACCTTCTTCAACAACATCCTGGTGCCCAACGCCAAGGAGAACTACGTGGCCAACCTGGAACTG
  CTCAACAACATCATCCAGTTCGATTGGGCCCCCCAGCTGTACCTCACATACGGGATCGCACAGGAGTCCT
  TCGGGCCTTTCGCCCAGCTGAACGACTGGACAATCCTGACCGGCGAGACCCTGGCCCGGATGCACGAGGT
  CTGCGTCACCTCCATGTTTGACGTGCCCCAAATGGGCTTTAATAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102087 representing YP_239360
  Red=Cloning sites Green=Tags
  
  MKQNDTKKTTQRRNSKKYSSKTNRGTKRAPRDQEVGTGAQESTRNDVAWYARYPHILEEATRLPFAYPIG
  QYYDTGYSVASATEWSKYVDTSLTIPGVMCVNFTPTPGESYNKNSPINIAAQNVYTYVRHMNSGHANYEQ
  ADLMMYLLAMDSLYIFHSYVRKILAISKLYTPVNKYFPRALLVALGVDPEDVFANQAQWEYFVNMVAYRA
  GAFAAPASMTYYERHAWMSNGLYVDQDVTRAQIYMFKPTMLWKYENLGTTGTKLVPLMMPKAGDNRKLVD
  FQVLFNNLVSTMLGDEDFGIMSGDVFKAFGADGLVKLLAVDSTTMTLPTYDPLILAQIHSARAVGAPILE
  TSTLTGFPGRQWQITQNPDVNNGAIIFHPSFGYDGQDHEELSFRAMCSNMILNLPGEAHSAEMIIEATRL
  ATMFQVKAVPAGDTSKPVLYLPNGFGTEVVNDYTMISVDKATPHDLTIHTFFNNILVPNAKENYVANLEL
  LNNIIQFDWAPQLYLTYGIAQESFGPFAQLNDWTILTGETLARMHEVCVTSMFDVPQMGFNK
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_007026
• ORF Size: 1656 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_007026.1, YP_239360
• RefSeq ORF: 1656
• Locus ID: 5075907
• MW: 62.0 kDa