NS2 (NC_001781) Virus Tagged ORF Clone

CAT#: VC102095

Myc-DDK-tagged ORF clone of viral ORF for non-structural protein 2 [Human respiratory syncytial virus], codon optimized for human cell expression, NP_056857

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102095 represents NCBI reference of NP_056857 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAACCACCAACGACAACACTACGATGCAAAGACTGATGATTACGGATATGAGACCACTGTCTATGG
  ATAGTATCATCACCTCCCTGACTAAAGAGATCATTACACATAAATTTATCTATCTGATCAACAACGAATG
  TATTGTGAGAAAACTGGACGAGCGACAAGCCACGTTTACATTCCTGGTTAATTACGAGATGAAACTGCTG
  CACAAAGTGGGGAGTACCAAATACAAGAAATATACCGAGTACAACACAAAATACGGAACTTTTCCTATGC
  CCATCTTTATAAACCATGGAGGTTTCCTCGAGTGCATTGGAATCAAGCCTACTAAACACACCCCTATCAT
  ATATAAATACGATCTGAATCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102095 representing NP_056857
  Red=Cloning sites Green=Tags
  
  MSTTNDNTTMQRLMITDMRPLSMDSIITSLTKEIITHKFIYLINNECIVRKLDERQATFTFLVNYEMKLL
  HKVGSTKYKKYTEYNTKYGTFPMPIFINHGGFLECIGIKPTKHTPIIYKYDLNP
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001781
• ORF Size: 372 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001781.1, NP_056857
• RefSeq ORF: 372
• Locus ID: 1489819
• MW: 14.6 kDa