M2 (NC_001781) Virus Tagged ORF Clone

CAT#: VC102102

Myc-DDK-tagged ORF clone of viral ORF for matrix protein 2 [Human respiratory syncytial virus], codon optimized for human cell expression, NP_056864

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: M2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102102 represents NCBI reference of NP_056864 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACGCCGGAACCCATGTAAATTCGAAATTAGAGGCCACTGTCTGAATGGGCGGAGGTGTCATTACA
  GCCACAACTATTTCGAATGGCCGCCTCATGCGCTCCTGGTGCGCCAGAATTTCATGCTGAATAAGATCCT
  CAAATCTATGGACAAGTCAATCGACACCCTGTCCGAAATTTCCGGCGCGGCGGAGCTGGACAGAACAGAG
  GAATATGCACTGGGCATAGTCGGGGTACTGGAGTCATATATCGGATCAATTAATAATATTACCAAGCAGA
  GTGCTTGTGTCGCAATGTCCAAGTTGCTTATCGAGATCAATTCCGATGATATCAAAAAGCTGCGCGATAA
  TGAAGAACCGAATAGTCCCAAAATCCGAGTGTACAATACAGTAATCTCCTACATTGAATCCAATCGCAAA
  AACAACAAGCAGACCATCCACCTGCTGAAACGCTTGCCTGCAGACGTCCTCAAGAAAACTATCAAAAATA
  CACTGGACATCCACAAGTCAATCATTATCAGCAATCCTAAAGAGAGCACAGTGAACGACCAAAACGATCA
  GACTAAGAATAACGATATCACCGGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102102 representing NP_056864
  Red=Cloning sites Green=Tags
  
  MSRRNPCKFEIRGHCLNGRRCHYSHNYFEWPPHALLVRQNFMLNKILKSMDKSIDTLSEISGAAELDRTE
  EYALGIVGVLESYIGSINNITKQSACVAMSKLLIEINSDDIKKLRDNEEPNSPKIRVYNTVISYIESNRK
  NNKQTIHLLKRLPADVLKKTIKNTLDIHKSIIISNPKESTVNDQNDQTKNNDITG
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001781
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001781.1, NP_056864
• RefSeq ORF: 585
• Locus ID: 1489826
• MW: 22.3 kDa