1B (VP2) (NC_001490) Virus Tagged ORF Clone

CAT#: VC102107

Myc-DDK-tagged ORF clone of viral ORF for 1B (VP2) [Human rhinovirus 14], codon optimized for human cell expression, NP_740516

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1B (VP2)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102107 represents NCBI reference of NP_740516 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCACCTAACGTAGAAGCATGCGGGTATTCAGACCGCGTCCAACAGATTACACTGGGGAATTCCACCA
  TTACCACACAGGAGGCCGCTAATGCCGTGGTTTGCTATGCTGAATGGCCAGAATACCTTCCCGACGTCGA
  TGCATCTGATGTGAACAAGACAAGCAAACCAGATACTTCAGTGTGTAGGTTCTACACGCTCGATTCCAAG
  ACATGGACTACAGGGTCCAAGGGGTGGTGTTGGAAGCTGCCAGACGCCCTGAAGGACATGGGCGTCTTTG
  GCCAAAATATGTTTTTTCACAGTCTGGGTAGATCCGGTTACACTGTGCACGTTCAGTGCAACGCTACCAA
  GTTTCACTCAGGATGTCTCCTGGTCGTGGTTATTCCTGAGCACCAACTGGCTAGCCACGAGGGAGGAAAC
  GTCTCCGTGAAATATACGTTCACTCATCCTGGCGAGAGAGGCATTGATCTGTCTTCAGCGAATGAGGTGG
  GCGGCCCGGTGAAAGACGTGATATACAATATGAACGGTACTCTGCTCGGCAATCTTCTTATTTTTCCACA
  CCAGTTCATTAACCTTAGGACCAACAACACCGCCACGATCGTAATTCCTTACATCAACAGTGTGCCTATC
  GACAGCATGACACGGCACAATAATGTTTCATTGATGGTGATCCCGATCGCCCCACTGACCGTGCCTACAG
  GGGCCACGCCCTCCCTGCCCATCACCGTGACGATCGCCCCAATGTGTACCGAGTTCAGTGGGATACGCAG
  TAAAAGTATCGTACCACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102107 representing NP_740516
  Red=Cloning sites Green=Tags
  
  MSPNVEACGYSDRVQQITLGNSTITTQEAANAVVCYAEWPEYLPDVDASDVNKTSKPDTSVCRFYTLDSK
  TWTTGSKGWCWKLPDALKDMGVFGQNMFFHSLGRSGYTVHVQCNATKFHSGCLLVVVIPEHQLASHEGGN
  VSVKYTFTHPGERGIDLSSANEVGGPVKDVIYNMNGTLLGNLLIFPHQFINLRTNNTATIVIPYINSVPI
  DSMTRHNNVSLMVIPIAPLTVPTGATPSLPITVTIAPMCTEFSGIRSKSIVPQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001490
• ORF Size: 789 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001490.1, NP_740516
• RefSeq ORF: 789
• MW: 28.6 kDa