1C (VP3) (NC_001490) Virus Tagged ORF Clone

CAT#: VC102108

Myc-DDK-tagged ORF clone of viral ORF for 1C (VP3) [Human rhinovirus 14], codon optimized for human cell expression, NP_740517

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: 1C (VP3)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102108 represents NCBI reference of NP_740517 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGATTGCCAACAACAACCCTGCCCGGATCCGGACAATTCCTGACCACCGACGACAGGCAGAGCCCTA
  GTGCTCTCCCAAATTACGAGCCAACCCCACGGATTCACATACTTGGCAAGGTTCACAACCTGCTTGAGAT
  CATTCAGGTGGATACCCTCATTCCGATGAATAACACCCACACCAAAGACGAAGTCAATTCCTATCTGATT
  CCCCTTAACGCAAACAGGCAGAATGAACAGGTGTTCGGAACCAACCTCTTCATAGGCGACGGGGTGTTTA
  AAACCACCCTGCTTGGGGAGATCGTTCAATACTACACCCACTGGAGCGGAAGTCTGCGATTTTCCTCCAT
  GTATACCGGGCCAGCACTTTCCTCCGCCAAATTGACCTTGGCTTATACACCCCCTGGCGCCCGCGGACCG
  CAAGACCGGAGGGAGGCCATGCTGGGAACCCACGTCGTGTGGGATATCGGATTGCAATCCACAATTGTTA
  TGACAATCCCATGGACTAGCGGTGTCCAATTCAGATACACCGACCCCGACACATATACTTCCGCTGGCTT
  CCTTTCCTGCTGGTACCAGACCTCCCTGATTCTGCCTCCTGAGACCACGGGGCAGGTGTACCTGCTGAGT
  TTTATCAGCGCTTGCCCCGACTTCAAGCTCCGCCTGATGAAGGATACCCAGACCATCTCACAGACTGTCG
  CCCTGACTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102108 representing NP_740517
  Red=Cloning sites Green=Tags
  
  MGLPTTTLPGSGQFLTTDDRQSPSALPNYEPTPRIHILGKVHNLLEIIQVDTLIPMNNTHTKDEVNSYLI
  PLNANRQNEQVFGTNLFIGDGVFKTTLLGEIVQYYTHWSGSLRFSSMYTGPALSSAKLTLAYTPPGARGP
  QDRREAMLGTHVVWDIGLQSTIVMTIPWTSGVQFRYTDPDTYTSAGFLSCWYQTSLILPPETTGQVYLLS
  FISACPDFKLRLMKDTQTISQTVALTE
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001490
• ORF Size: 711 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001490.1, NP_740517
• RefSeq ORF: 711
• MW: 26.3 kDa