P(M1) (NC_001652) Virus Tagged ORF Clone

CAT#: VC102270

Myc-DDK-tagged ORF clone of viral ORF for polymerase-associated protein [Infectious hematopoietic necrosis virus], codon optimized for human cell expression, NP_042677

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P(M1)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102270 represents NCBI reference of NP_042677 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCGATGGAGAGGGTGAGCAGTTTTTCAATCTCGAAGGCGAGGACATTTTGCGCCTGGAGAGCCGGT
  TGAAAACCCCCCGAAATGACGGTCAGATTGGCAAGAACCCCCGGAGACGCAAGGAGGATCAGGCACCTCA
  GGAGGAACCTAAGAAAACCACTCGAAGACCCGACAAGAATAAAGGCCTTTCTCAACTGGAACAGTTGATC
  CTGAAGTACGTAGAGGAGGAGAGCTGCCAAGATGCCCTGAAGGATTTCGGAGGACTGATTGCGAATATAC
  GACAAGCTCATCAGGCAGAGATGACCAGTCATCTCGAGAAGGTGGCCACAGAGCATCGGGCTAACCTCCA
  GGCCCTCACTAAAAGCCAGCAAGAACACGAGAAGGTGAGCAAGGAGATCCTGTCCGCTGTGATTGCCATT
  AGATCCAACCTGAATGAGAATCACTCTCCTCTGCCTAAGCCCCTGGATCCAGACCAGGTGAAAGCCGCTC
  GAGCCTTGGGCTTTGGGATTGGATACCGGACAGCTCTTAATGTGTTTGACCGAATCAAGGGGGTGACACC
  AGATAACGCTGGAAGCCAGGAAGTGAAAAATCTCGCCATTAGAGCTGCAGAGGAAGACGAATACGAGGGC
  TCACCCACTTTTTTTCGAAAGGTGATTGATGCCGTCAAGAAGCGAATGAAGCAGGGCCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102270 representing NP_042677
  Red=Cloning sites Green=Tags
  
  MSDGEGEQFFNLEGEDILRLESRLKTPRNDGQIGKNPRRRKEDQAPQEEPKKTTRRPDKNKGLSQLEQLI
  LKYVEEESCQDALKDFGGLIANIRQAHQAEMTSHLEKVATEHRANLQALTKSQQEHEKVSKEILSAVIAI
  RSNLNENHSPLPKPLDPDQVKAARALGFGIGYRTALNVFDRIKGVTPDNAGSQEVKNLAIRAAEEDEYEG
  SPTFFRKVIDAVKKRMKQGQ
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001652
• ORF Size: 690 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001652.1, NP_042677
• RefSeq ORF: 690
• Locus ID: 1489846
• MW: 25.9 kDa