G (NC_001652) Virus Tagged ORF Clone

CAT#: VC102272

Myc-DDK-tagged ORF clone of viral ORF for glycoprotein [Infectious hematopoietic necrosis virus], codon optimized for human cell expression, NP_042679

Specifications

Product Data

• Type: Virus Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: G
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >The Viral ORF clone VC102272 represents NCBI reference of NP_042679 with codon optimized for human cell expression
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  GACGTTGTATACGACTCCTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACACAATGATCACTACTCCTCTGATCTTGATCCTGATCACCTGTGGTGCTAACTCACAGACTGTGA
  AGCCCGATACCGCCTCAGAGTCCGATCAGCCCACGTGGAGCAACCCGTTGTTCACGTACCCTGAAGGTTG
  TACTCTCGACAAACTGTCTAAGGTGAACGCAAGTCAGCTTCGCTGTCCCAGAATCTTTGATGACGAGAAC
  CGCGGTCTGATTGCTTATCCAACGAGCATCCGCTCCTTGAGCGTGGGTAATGACTTGGGGGATATACATA
  CCCAGGGGAATCACATACACAAAGTGCTGTACCGGACTATTTGCAGCACCGGGTTTTTCGGAGGACAGAC
  GATCGAAAAGGCCCTCGTGAAGATGAAGCTGTCAACAAAGGAAGCTGGAGCGTACGACACGACCACCGCC
  GCCGCCCTCTACTTCCCCGCTCCTCGCTGCCAGTGGTACACCGACAACGTCCAGAACGACTTGATCTTTT
  ACTACACCACCCAAAAATCTGTGCTGCGGGATCCTTATACCAGGGACTTCCTCGATTCTGACTTTATCGG
  CGGAAAGTGTACAAAATCCCCCTGCCAGACACATTGGTCTAATGTCGTGTGGATGGGAGATGCTGGGATC
  CCTGCTTGCGATTCATCACAGGAGATCAAAGCCCACCTTTTCGTGGACAAGATTTCTAACAGGGTTGTGA
  AGGCCACCTCTTACGGCCACCACCCGTGGGGACTGCACCAGGCATGTATGATAGAATTCTGCGGCCAGCA
  ATGGATTCGGACAGATCTGGGAGATTTGATATCCGTGGTGTATAATTCCGGCAGCGAAATTCTCTCATTC
  CCTAAGTGTGAAGATAAGACCGTTGGGATGAGGGGCAACCTGGACGATTTCGCCTATCTTGATGATCTGG
  TGAAGGCAAGTGAAAGTCGAGAAGAATGTCTGGAGGCCCATGCCGAAATCATCTCCACGAATTCAGTGAC
  TCCTTATCTGCTGTCAAAGTTCAGGTCTCCACATCCAGGAATCAACGATGTGTACGCAATGCACAAGGGT
  AGTATCTATCACGGCATGTGCATGACCGTTGCAGTCGACGAAGTTTCTAAAGACAGAACCACATACAGGG
  CACATAGGGCCACCAGCTTTACCAAATGGGAACGACCTTTCGGGGATGAATGGGAGGGGTTTCACGGCTT
  GCACGGAAATAATACCACGATTATTCCCGATCTGGAGAAATACGTCGCTCAGTACAAGATGTCCATGATG
  GAGCCCATGAGTATCAAGTCTGTGCCACACCCCTCTATCCTGGCCCTGTATAATGAGACCGATGTCAGCG
  GGATATCTATCCGGAAGCTGGACTCTTTTGATTTGCAGAGCCTGCACTGGAGTTTTTGGCCCACCATTTC
  TGCACTCGGTGGCATACCTTTTGTGCTGTTGCTTGCTGTTGCCGCCTGTTGCTGTTGGTCCGGTAGGCCC
  CCTACTCCTTCAGTCCCTCAGTCTATCCCTATGTACCATCTCGCAAACAGGAGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >VC102272 representing NP_042679
  Red=Cloning sites Green=Tags
  
  MDTMITTPLILILITCGANSQTVKPDTASESDQPTWSNPLFTYPEGCTLDKLSKVNASQLRCPRIFDDEN
  RGLIAYPTSIRSLSVGNDLGDIHTQGNHIHKVLYRTICSTGFFGGQTIEKALVKMKLSTKEAGAYDTTTA
  AALYFPAPRCQWYTDNVQNDLIFYYTTQKSVLRDPYTRDFLDSDFIGGKCTKSPCQTHWSNVVWMGDAGI
  PACDSSQEIKAHLFVDKISNRVVKATSYGHHPWGLHQACMIEFCGQQWIRTDLGDLISVVYNSGSEILSF
  PKCEDKTVGMRGNLDDFAYLDDLVKASESREECLEAHAEIISTNSVTPYLLSKFRSPHPGINDVYAMHKG
  SIYHGMCMTVAVDEVSKDRTTYRAHRATSFTKWERPFGDEWEGFHGLHGNNTTIIPDLEKYVAQYKMSMM
  EPMSIKSVPHPSILALYNETDVSGISIRKLDSFDLQSLHWSFWPTISALGGIPFVLLLAVAACCCWSGRP
  PTPSVPQSIPMYHLANRS
  
  TRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NC_001652
• ORF Size: 1524 bp
• OTI Disclaimer: The molecular sequence of this clone can be viewed by clicking the "ORF Nucleotide Sequence" link above. This sequence represents the NCBI reference after codon optimization for human cell expression, and retaining the same decuded protein sequence. The stop codon in the native sequence was removed to create the in-frame c-terminal fusion with a Myc-DDK tag.

Reference Data

• RefSeq: NC_001652.1, NP_042679
• RefSeq ORF: 1524
• Locus ID: 1489848
• MW: 56.8 kDa